LOCUS BBa_K132008 4104 bp DNA linear 22-Dec-2024 DEFINITION kanR-LVA+lacI+PL+kanR-LVA+aiiA+terminator ACCESSION BBa_K132008 VERSION BBa_K132008.1 FEATURES Location/Qualifiers RBS 1..12 /label=RBS misc_feature 13..18 /label=scar misc_feature 18..831 /label=kanR gene misc_feature 832..864 /label=LVA misc_feature 865..870 /label=stop misc_feature 1..870 /label=BBa_K132000 misc_feature 883..886 /label=conserved RBS 879..890 /label=BBa_B0034 misc_feature 1986..2024 /label=LVA CDS 897..2024 /label=lacI-LVA misc_feature 897..2024 /label=BBa_C0012 misc_feature 2025..2049 /label=Help:Barcodes CDS 897..2024 /label=BBa_C0012 stem_loop 2069..2112 /label=stem_loop misc_feature 2058..2137 /label=BBa_B0010 terminator 2058..2137 /label=BBa_B0010 stem_loop 2153..2172 /label=T7 TE misc_feature 2179..2179 /label=stop polyA_site 2173..2186 /label=polya misc_feature 2146..2186 /label=BBa_B0012 terminator 2146..2186 /label=BBa_B0012 terminator 2058..2186 /label=BBa_B0015 misc_feature 2197..2213 /label=lac O1 promoter 2214..2219 /label=-35 misc_feature 2220..2236 /label=lac O1 promoter 2237..2242 /label=-10 misc_feature 2195..2248 /label=BBa_R0011 promoter 2195..2248 /label=BBa_R0011 misc_feature 879..2249 /label=BBa_Q04121 misc_feature 1..2249 /label=BBa_K132003 RBS 2258..2269 /label=RBS misc_feature 2270..2275 /label=scar misc_feature 2275..3088 /label=kanR gene misc_feature 3089..3121 /label=LVA misc_feature 3122..3127 /label=stop misc_feature 2258..3127 /label=BBa_K132000 misc_feature 1..3127 /label=BBa_K132007 misc_feature 3140..3143 /label=conserved RBS 3136..3147 /label=BBa_B0034 misc_feature 3154..3156 /label=start misc_feature 3937..3942 /label=2 misc_feature 3904..3936 /label=LVA CDS 3154..3903 /label=aiiA misc_feature 3154..3942 /label=BBa_C0060 misc_feature 3943..3967 /label=Help:Barcodes CDS 3154..3942 /label=BBa_C0060 stem_loop 3987..4030 /label=stem_loop misc_feature 3976..4055 /label=BBa_B0010 terminator 3976..4055 /label=BBa_B0010 stem_loop 4071..4090 /label=T7 TE misc_feature 4097..4097 /label=stop polyA_site 4091..4104 /label=polya misc_feature 4064..4104 /label=BBa_B0012 terminator 4064..4104 /label=BBa_B0012 misc_feature 3136..4104 /label=BBa_I0460 ORIGIN 1 aaagaggaga aatactagat gagccatatt caacgggaaa cgtcttgctc caggccgcga 61 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 121 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 181 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 241 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 301 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct 361 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 421 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 481 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 541 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc 601 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 661 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 721 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 781 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt cgctgcaaac 841 gacgaaaact acgctttagt agcttaataa tactagagaa agaggagaaa tactagatgg 901 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 961 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 1021 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 1081 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 1141 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 1201 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 1261 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 1321 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 1381 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 1441 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 1501 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 1561 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 1621 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 1681 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 1741 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 1801 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 1861 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 1921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 1981 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt 2041 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2101 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2161 ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa caattgacat 2221 tgtgagcgga taacaagata ctgagcacat actagagaaa gaggagaaat actagatgag 2281 ccatattcaa cgggaaacgt cttgctccag gccgcgatta aattccaaca tggatgctga 2341 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 2401 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 2461 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 2521 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 2581 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 2641 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 2701 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 2761 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 2821 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 2881 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 2941 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 3001 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 3061 gtttcatttg atgctcgatg agtttttcgc tgcaaacgac gaaaactacg ctttagtagc 3121 ttaataatac tagagaaaga ggagaaatac tagatgacag taaagaagct ttatttcgtc 3181 ccagcaggtc gttgtatgtt ggatcattcg tctgttaata gtacattaac accaggagaa 3241 ttattagact taccggtttg gtgttatctt ttggagactg aagaaggacc tattttagta 3301 gatacaggta tgccagaaag tgcagttaat aatgaaggtc tttttaacgg tacatttgtc 3361 gaagggcagg ttttaccgaa aatgactgaa gaagatagaa tcgtgaatat tttaaaacgg 3421 gttggttatg agccggaaga ccttctttat attattagtt ctcacttgca ttttgatcat 3481 gcaggaggaa atggcgcttt tataaataca ccaatcattg tacagcgtgc tgaatatgag 3541 gcggcgcagc atagcgaaga atatttgaaa gaatgtatat tgccgaattt aaactacaaa 3601 atcattgaag gtgattatga agtcgtacca ggagttcaat tattgcatac accaggccat 3661 actccagggc atcaatcgct attaattgag acagaaaaat ccggtcctgt attattaacg 3721 attgatgcat cgtatacgaa agagaatttt gaaaatgaag tgccatttgc gggatttgat 3781 tcagaattag ctttatcttc aattaaacgt ttaaaagaag tggtgatgaa agagaagccg 3841 attgttttct ttggacatga tatagagcag gaaaggggat gtaaagtgtt ccctgaatat 3901 atagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 3961 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 4021 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 4081 cgggtgggcc tttctgcgtt tata //