##gff-version 3
##sequence-region BBa_K132008 1 4104
BBa_K132008	.	engineered_region	3136	4104	.	+	0	ID=BBa_I0460;Name=BBa_I0460
BBa_K132008	.	ribosome_entry_site	3136	3147	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K132008	.	conserved_region	3140	3143	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132008	.	terminator	3976	4055	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K132008	.	stem_loop	3987	4030	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132008	.	engineered_region	3976	4055	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132008	.	terminator	4064	4104	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K132008	.	engineered_region	4064	4104	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132008	.	stop_codon	4097	4097	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132008	.	stem_loop	4071	4090	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132008	.	polyA_site	4091	4104	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132008	.	CDS	3154	3942	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K132008	.	stop_codon	3937	3942	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K132008	.	start_codon	3154	3156	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K132008	.	engineered_region	3154	3942	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K132008	.	engineered_tag	3943	3967	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K132008	.	tag	3904	3936	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K132008	.	CDS	3154	3903	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K132008	.	engineered_region	1	3127	.	+	0	ID=BBa_K132007;Name=BBa_K132007
BBa_K132008	.	engineered_region	1	2249	.	+	0	ID=BBa_K132003;Name=BBa_K132003;Parent=BBa_K132007
BBa_K132008	.	engineered_region	879	2249	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_K132003
BBa_K132008	.	terminator	2058	2186	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K132008	.	terminator	2058	2137	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132008	.	stem_loop	2069	2112	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132008	.	engineered_region	2058	2137	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132008	.	terminator	2146	2186	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132008	.	engineered_region	2146	2186	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132008	.	stop_codon	2179	2179	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132008	.	stem_loop	2153	2172	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132008	.	polyA_site	2173	2186	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132008	.	promoter	2195	2248	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K132008	.	promoter	2214	2219	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K132008	.	promoter	2237	2242	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K132008	.	operator	2197	2213	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K132008	.	operator	2220	2236	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K132008	.	engineered_region	2195	2248	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K132008	.	CDS	897	2024	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K132008	.	engineered_tag	2025	2049	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K132008	.	CDS	897	2024	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K132008	.	tag	1986	2024	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K132008	.	engineered_region	897	2024	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K132008	.	ribosome_entry_site	879	890	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K132008	.	conserved_region	883	886	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132008	.	engineered_region	1	870	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132003
BBa_K132008	.	tag	832	864	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132008	.	engineered_region	18	831	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132008	.	sequence_feature	13	18	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132008	.	stop_codon	865	870	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132008	.	ribosome_entry_site	1	12	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132008	.	engineered_region	2258	3127	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132007
BBa_K132008	.	tag	3089	3121	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132008	.	engineered_region	2275	3088	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132008	.	sequence_feature	2270	2275	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132008	.	stop_codon	3122	3127	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132008	.	ribosome_entry_site	2258	2269	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
>BBa_K132008
aaagaggagaaatactagatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatg
ctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcc
cgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaac
tggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactg
cgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagt
gttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcg
caatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtct
ggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttat
ttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatc
ctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgata
tgaataaattgcagtttcatttgatgctcgatgagtttttcgctgcaaacgacgaaaactacgctttagtagcttaata
atactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtg
tctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggc
gatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgcc
acctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcg
tggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcag
tgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtta
tttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagc
atctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggc
tggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggt
tttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgg
gcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaaga
cagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttg
ctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctgg
cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgga
aagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataaca
attgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagccatattcaac
gggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataa
tgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaa
ggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatca
agcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattaga
agaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgt
aattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcga
gtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcacc
ggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgat
gttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcat
tacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatga
gtttttcgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatgacag
taaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggaga
attattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaa
agtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagata
gaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttga
tcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagc
gaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggag
ttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtatt
attaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagct
ttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcagg
aaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata