LOCUS BBa_K132012 3658 bp DNA linear 22-Nov-2024 DEFINITION cmR+araC+Pbad/araC+cmR+aiiA ACCESSION BBa_K132012 VERSION BBa_K132012.1 FEATURES Location/Qualifiers RBS 1..12 /label=RBS misc_feature 13..20 /label=scar CDS 20..677 /label=cmR gene misc_feature 677..710 /label=LVA misc_feature 710..716 /label=stop misc_feature 1..716 /label=BBa_K132001 misc_feature 729..732 /label=conserved RBS 725..736 /label=BBa_B0034 CDS 743..1618 /label=araC misc_feature 1619..1651 /label=LVA misc_feature 1658..1682 /label=Help:Barcodes CDS 743..1682 /label=BBa_C0080 misc_feature 725..1682 /label=BBa_S03519 stem_loop 1702..1745 /label=stem_loop misc_feature 1691..1770 /label=BBa_B0010 terminator 1691..1770 /label=BBa_B0010 stem_loop 1786..1805 /label=T7 TE misc_feature 1812..1812 /label=stop polyA_site 1806..1819 /label=polya misc_feature 1779..1819 /label=BBa_B0012 terminator 1779..1819 /label=BBa_B0012 terminator 1691..1819 /label=BBa_B0015 misc_feature 725..1819 /label=BBa_K132018 promoter 1828..1957 /label=BBa_I13453 RBS 1966..1977 /label=RBS misc_feature 1978..1985 /label=scar CDS 1985..2642 /label=cmR gene misc_feature 2642..2675 /label=LVA misc_feature 2675..2681 /label=stop misc_feature 1966..2681 /label=BBa_K132001 misc_feature 725..2681 /label=BBa_K132020 misc_feature 2694..2697 /label=conserved RBS 2690..2701 /label=BBa_B0034 misc_feature 2708..2710 /label=start misc_feature 3491..3496 /label=2 misc_feature 3458..3490 /label=LVA CDS 2708..3457 /label=aiiA misc_feature 2708..3496 /label=BBa_C0060 misc_feature 3497..3521 /label=Help:Barcodes CDS 2708..3496 /label=BBa_C0060 stem_loop 3541..3584 /label=stem_loop misc_feature 3530..3609 /label=BBa_B0010 terminator 3530..3609 /label=BBa_B0010 stem_loop 3625..3644 /label=T7 TE misc_feature 3651..3651 /label=stop polyA_site 3645..3658 /label=polya misc_feature 3618..3658 /label=BBa_B0012 terminator 3618..3658 /label=BBa_B0012 misc_feature 2690..3658 /label=BBa_I0460 ORIGIN 1 aaagaggaga aatactagag atggagaaaa aaatcactgg atataccacc gttgatatat 61 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc 241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 421 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 601 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 661 agtggcaggg cggggcggct gcaaacgacg aaaactacgc tttagtagct taataatact 721 agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc tgccgggata 781 ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga 841 tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg 901 tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct 961 gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca 1021 ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat 1081 atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct 1141 gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat 1201 aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc 1261 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag 1321 caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca 1381 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag 1441 ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt 1501 tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc 1561 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc 1621 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 1681 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1801 gggcctttct gcgtttatat actagagaca ttgattattt gcacggcgtc acactttgct 1861 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 1921 tctctactgt ttctccatac cgtttttttg ggctagctac tagagaaaga ggagaaatac 1981 tagagatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 2041 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 2101 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 2161 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag 2221 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 2281 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 2341 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 2401 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 2461 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 2521 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 2581 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 2641 cggctgcaaa cgacgaaaac tacgctttag tagcttaata atactagaga aagaggagaa 2701 atactagatg acagtaaaga agctttattt cgtcccagca ggtcgttgta tgttggatca 2761 ttcgtctgtt aatagtacat taacaccagg agaattatta gacttaccgg tttggtgtta 2821 tcttttggag actgaagaag gacctatttt agtagataca ggtatgccag aaagtgcagt 2881 taataatgaa ggtcttttta acggtacatt tgtcgaaggg caggttttac cgaaaatgac 2941 tgaagaagat agaatcgtga atattttaaa acgggttggt tatgagccgg aagaccttct 3001 ttatattatt agttctcact tgcattttga tcatgcagga ggaaatggcg cttttataaa 3061 tacaccaatc attgtacagc gtgctgaata tgaggcggcg cagcatagcg aagaatattt 3121 gaaagaatgt atattgccga atttaaacta caaaatcatt gaaggtgatt atgaagtcgt 3181 accaggagtt caattattgc atacaccagg ccatactcca gggcatcaat cgctattaat 3241 tgagacagaa aaatccggtc ctgtattatt aacgattgat gcatcgtata cgaaagagaa 3301 ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa ttagctttat cttcaattaa 3361 acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt ttctttggac atgatataga 3421 gcaggaaagg ggatgtaaag tgttccctga atatatagct gcaaacgacg aaaactacgc 3481 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 3541 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 3601 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //