##gff-version 3
##sequence-region BBa_K132013 1 2152
BBa_K132013	.	engineered_region	1	1083	.	+	0	ID=BBa_J04451;Name=BBa_J04451
BBa_K132013	.	terminator	1043	1083	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04451
BBa_K132013	.	polyA_site	1070	1083	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132013	.	stem_loop	1050	1069	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132013	.	engineered_region	1043	1083	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132013	.	stop_codon	1076	1076	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132013	.	engineered_region	227	946	.	+	0	ID=BBa_J04051;Name=BBa_J04051;Parent=BBa_J04451
BBa_K132013	.	tag	902	940	.	+	0	ID=annotation1507968;Name=LVA;Parent=BBa_J04051
BBa_K132013	.	start_codon	227	229	.	+	0	ID=annotation1508160;Name=Start;Parent=BBa_J04051
BBa_K132013	.	stop_codon	941	946	.	+	0	ID=annotation1507863;Name=Stop;Parent=BBa_J04051
BBa_K132013	.	CDS	227	901	.	+	0	ID=annotation1507347;Name=mrfp1;Parent=BBa_J04051
BBa_K132013	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04451
BBa_K132013	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132013	.	terminator	955	1034	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04451
BBa_K132013	.	stem_loop	966	1009	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132013	.	engineered_region	955	1034	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132013	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04451
BBa_K132013	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K132013	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K132013	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K132013	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K132013	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K132013	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K132013	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K132013	.	engineered_region	1092	2152	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_K132013	.	promoter	1092	1145	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K132013	.	promoter	1111	1116	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K132013	.	promoter	1134	1139	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K132013	.	non_covalent_binding_site	1092	1110	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K132013	.	engineered_region	1092	1145	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K132013	.	non_covalent_binding_site	1117	1135	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K132013	.	ribosome_entry_site	1154	1165	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K132013	.	conserved_region	1158	1161	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132013	.	terminator	1961	2089	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K132013	.	terminator	1961	2040	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132013	.	stem_loop	1972	2015	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132013	.	engineered_region	1961	2040	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132013	.	terminator	2049	2089	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132013	.	polyA_site	2076	2089	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132013	.	stem_loop	2056	2075	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132013	.	engineered_region	2049	2089	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132013	.	stop_codon	2082	2082	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132013	.	promoter	2098	2152	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K132013	.	promoter	2117	2122	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K132013	.	non_covalent_binding_site	2098	2117	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K132013	.	promoter	2139	2144	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K132013	.	engineered_region	2098	2152	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K132013	.	start_codon	2150	2150	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K132013	.	CDS	1172	1927	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K132013	.	engineered_region	1172	1927	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K132013	.	engineered_tag	1928	1952	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K132013	.	CDS	1172	1921	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K132013	.	point_mutation	1345	1345	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K132013	.	engineered_region	1172	1173	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K132013	.	point_mutation	1663	1663	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_K132013
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgctaggcctgctgcaaacgacgaaaactacgctttagtagcttaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaa
tgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgat
atgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaa
tcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagatta
ttctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaatt
aaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgctta
gttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcc
ttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaa
tgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactt
tccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagc
aattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatgg
tttgttatagtcgaataaa