LOCUS BBa_K132015 2663 bp DNA linear 25-Nov-2024 DEFINITION Ptet+cfp+Ptet+rhlR+Prhl+luxI ACCESSION BBa_K132015 VERSION BBa_K132015.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..842 /label=CFP (LVA) misc_feature 799..836 /label=SsrA misc_feature 837..842 /label=2 misc_feature 149..149 /label=A misc_feature 81..842 /label=BBa_E0022 misc_feature 81..842 /label=BBa_E0022 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 misc_feature 988..1006 /label=TetR 1 misc_feature 988..1041 /label=BBa_R0040 promoter 1007..1012 /label=-35 misc_feature 1013..1031 /label=TetR 2 promoter 1030..1035 /label=-10 promoter 988..1041 /label=BBa_R0040 misc_feature 1..1041 /label=BBa_K132009 misc_feature 1054..1057 /label=conserved RBS 1050..1061 /label=BBa_B0034 CDS 1068..1796 /label=BBa_C0171 stem_loop 1816..1859 /label=stem_loop misc_feature 1805..1884 /label=BBa_B0010 terminator 1805..1884 /label=BBa_B0010 stem_loop 1900..1919 /label=T7 TE misc_feature 1926..1926 /label=stop polyA_site 1920..1933 /label=polya misc_feature 1893..1933 /label=BBa_B0012 terminator 1893..1933 /label=BBa_B0012 misc_feature 1942..1961 /label=RhlR promoter 1960..1965 /label=-35 promoter 1983..1988 /label=-10 misc_feature 1994..1994 /label=start promoter 1942..1994 /label=BBa_R0071 misc_feature 1050..1994 /label=BBa_K132010 misc_feature 2007..2010 /label=conserved RBS 2003..2014 /label=BBa_B0034 misc_feature 2600..2631 /label=LVA CDS 2021..2599 /label=luxI misc_feature 2021..2638 /label=BBa_C0061 misc_feature 2639..2663 /label=Help:Barcodes CDS 2021..2638 /label=BBa_C0061 misc_feature 2003..2663 /label=BBa_C0261 misc_feature 1050..2663 /label=BBa_K132011 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg 181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc 301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca 541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 781 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 1021 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg aggaatgacg 1081 gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc 1141 agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct 1201 atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc 1261 ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc 1321 tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc 1381 ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc 1441 gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca 1501 gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga 1561 agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg 1621 aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc 1681 tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg 1741 cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc taataatact 1801 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1861 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1921 ttctgcgttt atatactaga gtcctgtgaa atctggcagt taccgttagc tttcgaattg 1981 gctaaaaagt gttctactag agaaagagga gaaatactag atgactataa tgataaaaaa 2041 atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca 2101 agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga 2161 gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg 2221 atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt 2281 gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt 2341 aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga 2401 agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac 2461 agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga 2521 aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt 2581 taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact 2641 ctgatagtgc tagtgtagat ctc //