##gff-version 3
##sequence-region BBa_K132015 1 2663
BBa_K132015	.	engineered_region	1	1041	.	+	0	ID=BBa_K132009;Name=BBa_K132009
BBa_K132015	.	promoter	988	1041	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K132009
BBa_K132015	.	non_covalent_binding_site	1013	1031	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K132015	.	engineered_region	988	1041	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K132015	.	promoter	1007	1012	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K132015	.	non_covalent_binding_site	988	1006	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K132015	.	promoter	1030	1035	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K132015	.	engineered_region	81	842	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_K132009
BBa_K132015	.	tag	799	836	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K132015	.	CDS	81	842	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K132015	.	engineered_region	81	842	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K132015	.	point_mutation	149	149	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K132015	.	stop_codon	837	842	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K132015	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K132009
BBa_K132015	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132015	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K132009
BBa_K132015	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132015	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132015	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K132009
BBa_K132015	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K132015	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K132015	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K132015	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K132015	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K132015	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K132009
BBa_K132015	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132015	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132015	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132015	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132015	.	engineered_region	1050	2663	.	+	0	ID=BBa_K132011;Name=BBa_K132011
BBa_K132015	.	engineered_region	2003	2663	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K132011
BBa_K132015	.	ribosome_entry_site	2003	2014	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K132015	.	conserved_region	2007	2010	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132015	.	CDS	2021	2638	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K132015	.	CDS	2021	2599	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K132015	.	tag	2600	2631	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K132015	.	engineered_tag	2639	2663	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K132015	.	engineered_region	2021	2638	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K132015	.	engineered_region	1050	1994	.	+	0	ID=BBa_K132010;Name=BBa_K132010;Parent=BBa_K132011
BBa_K132015	.	promoter	1942	1994	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K132010
BBa_K132015	.	promoter	1983	1988	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K132015	.	non_covalent_binding_site	1942	1961	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K132015	.	promoter	1960	1965	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K132015	.	start_codon	1994	1994	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K132015	.	terminator	1805	1884	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K132010
BBa_K132015	.	engineered_region	1805	1884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132015	.	stem_loop	1816	1859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132015	.	ribosome_entry_site	1050	1061	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K132010
BBa_K132015	.	conserved_region	1054	1057	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132015	.	terminator	1893	1933	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K132010
BBa_K132015	.	engineered_region	1893	1933	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132015	.	polyA_site	1920	1933	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132015	.	stop_codon	1926	1926	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132015	.	stem_loop	1900	1919	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132015	.	CDS	1068	1796	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K132010
>BBa_K132015
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcactactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggt
ttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggct
tcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaa
ggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaa
atggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcg
gcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccag
cttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcat
ccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagtt
ccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcga
cgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcg
aattggctaaaaagtgttctactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttgg
caattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagt
tgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaat
gtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaac
agagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactc
tgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaaca
gtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatg
tattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc