LOCUS BBa_K132017 4773 bp DNA linear 22-Dec-2024 DEFINITION luxI+kanR-LVA+lacI+PL+kanR-LVA+aiiA+terminator ACCESSION BBa_K132017 VERSION BBa_K132017.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 misc_feature 1..661 /label=BBa_C0261 RBS 670..681 /label=RBS misc_feature 682..687 /label=scar misc_feature 687..1500 /label=kanR gene misc_feature 1501..1533 /label=LVA misc_feature 1534..1539 /label=stop misc_feature 670..1539 /label=BBa_K132000 misc_feature 1552..1555 /label=conserved RBS 1548..1559 /label=BBa_B0034 misc_feature 2655..2693 /label=LVA CDS 1566..2693 /label=lacI-LVA misc_feature 1566..2693 /label=BBa_C0012 misc_feature 2694..2718 /label=Help:Barcodes CDS 1566..2693 /label=BBa_C0012 stem_loop 2738..2781 /label=stem_loop misc_feature 2727..2806 /label=BBa_B0010 terminator 2727..2806 /label=BBa_B0010 stem_loop 2822..2841 /label=T7 TE misc_feature 2848..2848 /label=stop polyA_site 2842..2855 /label=polya misc_feature 2815..2855 /label=BBa_B0012 terminator 2815..2855 /label=BBa_B0012 terminator 2727..2855 /label=BBa_B0015 misc_feature 2866..2882 /label=lac O1 promoter 2883..2888 /label=-35 misc_feature 2889..2905 /label=lac O1 promoter 2906..2911 /label=-10 misc_feature 2864..2917 /label=BBa_R0011 promoter 2864..2917 /label=BBa_R0011 misc_feature 1548..2918 /label=BBa_Q04121 misc_feature 670..2918 /label=BBa_K132003 RBS 2927..2938 /label=RBS misc_feature 2939..2944 /label=scar misc_feature 2944..3757 /label=kanR gene misc_feature 3758..3790 /label=LVA misc_feature 3791..3796 /label=stop misc_feature 2927..3796 /label=BBa_K132000 misc_feature 670..3796 /label=BBa_K132007 misc_feature 3809..3812 /label=conserved RBS 3805..3816 /label=BBa_B0034 misc_feature 3823..3825 /label=start misc_feature 4606..4611 /label=2 misc_feature 4573..4605 /label=LVA CDS 3823..4572 /label=aiiA misc_feature 3823..4611 /label=BBa_C0060 misc_feature 4612..4636 /label=Help:Barcodes CDS 3823..4611 /label=BBa_C0060 stem_loop 4656..4699 /label=stem_loop misc_feature 4645..4724 /label=BBa_B0010 terminator 4645..4724 /label=BBa_B0010 stem_loop 4740..4759 /label=T7 TE misc_feature 4766..4766 /label=stop polyA_site 4760..4773 /label=polya misc_feature 4733..4773 /label=BBa_B0012 terminator 4733..4773 /label=BBa_B0012 misc_feature 3805..4773 /label=BBa_I0460 misc_feature 670..4773 /label=BBa_K132008 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagaga aagaggagaa atactagatg agccatattc aacgggaaac gtcttgctcc 721 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 781 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag 841 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 901 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 961 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa 1021 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 1081 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 1141 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 1201 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat 1261 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 1321 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 1381 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 1441 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 1501 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagaaa gaggagaaat 1561 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 1621 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 1681 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 1741 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 1801 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 1861 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 1921 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 1981 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 2041 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 2101 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 2161 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 2221 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 2281 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 2341 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 2401 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 2461 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 2521 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 2581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2641 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 2701 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2761 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2821 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac 2881 aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata 2941 ctagatgagc catattcaac gggaaacgtc ttgctccagg ccgcgattaa attccaacat 3001 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac 3061 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg 3121 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat 3181 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac 3241 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa 3301 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg 3361 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg 3421 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg 3481 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt 3541 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg 3601 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt 3661 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa 3721 taaattgcag tttcatttga tgctcgatga gtttttcgct gcaaacgacg aaaactacgc 3781 tttagtagct taataatact agagaaagag gagaaatact agatgacagt aaagaagctt 3841 tatttcgtcc cagcaggtcg ttgtatgttg gatcattcgt ctgttaatag tacattaaca 3901 ccaggagaat tattagactt accggtttgg tgttatcttt tggagactga agaaggacct 3961 attttagtag atacaggtat gccagaaagt gcagttaata atgaaggtct ttttaacggt 4021 acatttgtcg aagggcaggt tttaccgaaa atgactgaag aagatagaat cgtgaatatt 4081 ttaaaacggg ttggttatga gccggaagac cttctttata ttattagttc tcacttgcat 4141 tttgatcatg caggaggaaa tggcgctttt ataaatacac caatcattgt acagcgtgct 4201 gaatatgagg cggcgcagca tagcgaagaa tatttgaaag aatgtatatt gccgaattta 4261 aactacaaaa tcattgaagg tgattatgaa gtcgtaccag gagttcaatt attgcataca 4321 ccaggccata ctccagggca tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta 4381 ttattaacga ttgatgcatc gtatacgaaa gagaattttg aaaatgaagt gccatttgcg 4441 ggatttgatt cagaattagc tttatcttca attaaacgtt taaaagaagt ggtgatgaaa 4501 gagaagccga ttgttttctt tggacatgat atagagcagg aaaggggatg taaagtgttc 4561 cctgaatata tagctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 4621 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 4681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 4741 gctcaccttc gggtgggcct ttctgcgttt ata // LOCUS BBa_K132016 4773 bp DNA linear 22-Dec-2024 DEFINITION luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator ACCESSION BBa_K132016 VERSION BBa_K132016.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 RBS 670..681 /label=RBS misc_feature 682..687 /label=scar misc_feature 687..1500 /label=kanR gene misc_feature 1501..1533 /label=LVA misc_feature 1534..1539 /label=stop misc_feature 670..1539 /label=BBa_K132000 misc_feature 1552..1555 /label=conserved RBS 1548..1559 /label=BBa_B0034 misc_feature 2655..2693 /label=LVA CDS 1566..2693 /label=lacI-LVA misc_feature 1566..2693 /label=BBa_C0012 misc_feature 2694..2718 /label=Help:Barcodes CDS 1566..2693 /label=BBa_C0012 stem_loop 2738..2781 /label=stem_loop misc_feature 2727..2806 /label=BBa_B0010 terminator 2727..2806 /label=BBa_B0010 stem_loop 2822..2841 /label=T7 TE misc_feature 2848..2848 /label=stop polyA_site 2842..2855 /label=polya misc_feature 2815..2855 /label=BBa_B0012 terminator 2815..2855 /label=BBa_B0012 misc_feature 2866..2882 /label=lac O1 promoter 2883..2888 /label=-35 misc_feature 2889..2905 /label=lac O1 promoter 2906..2911 /label=-10 misc_feature 2864..2917 /label=BBa_R0011 promoter 2864..2917 /label=BBa_R0011 RBS 2927..2938 /label=RBS misc_feature 2939..2944 /label=scar misc_feature 2944..3757 /label=kanR gene misc_feature 3758..3790 /label=LVA misc_feature 3791..3796 /label=stop misc_feature 2927..3796 /label=BBa_K132000 misc_feature 3809..3812 /label=conserved RBS 3805..3816 /label=BBa_B0034 misc_feature 3823..3825 /label=start misc_feature 4606..4611 /label=2 misc_feature 4573..4605 /label=LVA CDS 3823..4572 /label=aiiA misc_feature 3823..4611 /label=BBa_C0060 misc_feature 4612..4636 /label=Help:Barcodes CDS 3823..4611 /label=BBa_C0060 stem_loop 4656..4699 /label=stem_loop misc_feature 4645..4724 /label=BBa_B0010 terminator 4645..4724 /label=BBa_B0010 stem_loop 4740..4759 /label=T7 TE misc_feature 4766..4766 /label=stop polyA_site 4760..4773 /label=polya misc_feature 4733..4773 /label=BBa_B0012 terminator 4733..4773 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagaga aagaggagaa atactagatg agccatattc aacgggaaac gtcttgctcc 721 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 781 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag 841 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 901 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 961 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa 1021 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 1081 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 1141 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 1201 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat 1261 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 1321 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 1381 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 1441 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 1501 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagaaa gaggagaaat 1561 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 1621 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 1681 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 1741 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 1801 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 1861 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 1921 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 1981 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 2041 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 2101 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 2161 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 2221 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 2281 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 2341 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 2401 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 2461 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 2521 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 2581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2641 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 2701 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2761 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2821 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac 2881 aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata 2941 ctagatgagc catattcaac gggaaacgtc ttgctccagg ccgcgattaa attccaacat 3001 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac 3061 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg 3121 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat 3181 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac 3241 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa 3301 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg 3361 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg 3421 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg 3481 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt 3541 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg 3601 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt 3661 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa 3721 taaattgcag tttcatttga tgctcgatga gtttttcgct gcaaacgacg aaaactacgc 3781 tttagtagct taataatact agagaaagag gagaaatact agatgacagt aaagaagctt 3841 tatttcgtcc cagcaggtcg ttgtatgttg gatcattcgt ctgttaatag tacattaaca 3901 ccaggagaat tattagactt accggtttgg tgttatcttt tggagactga agaaggacct 3961 attttagtag atacaggtat gccagaaagt gcagttaata atgaaggtct ttttaacggt 4021 acatttgtcg aagggcaggt tttaccgaaa atgactgaag aagatagaat cgtgaatatt 4081 ttaaaacggg ttggttatga gccggaagac cttctttata ttattagttc tcacttgcat 4141 tttgatcatg caggaggaaa tggcgctttt ataaatacac caatcattgt acagcgtgct 4201 gaatatgagg cggcgcagca tagcgaagaa tatttgaaag aatgtatatt gccgaattta 4261 aactacaaaa tcattgaagg tgattatgaa gtcgtaccag gagttcaatt attgcataca 4321 ccaggccata ctccagggca tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta 4381 ttattaacga ttgatgcatc gtatacgaaa gagaattttg aaaatgaagt gccatttgcg 4441 ggatttgatt cagaattagc tttatcttca attaaacgtt taaaagaagt ggtgatgaaa 4501 gagaagccga ttgttttctt tggacatgat atagagcagg aaaggggatg taaagtgttc 4561 cctgaatata tagctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 4621 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 4681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 4741 gctcaccttc gggtgggcct ttctgcgttt ata //