##gff-version 3
##sequence-region BBa_K132017 1 4773
BBa_K132017	.	engineered_region	670	4773	.	+	0	ID=BBa_K132008;Name=BBa_K132008
BBa_K132017	.	engineered_region	3805	4773	.	+	0	ID=BBa_I0460;Name=BBa_I0460;Parent=BBa_K132008
BBa_K132017	.	terminator	4645	4724	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K132017	.	stem_loop	4656	4699	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132017	.	engineered_region	4645	4724	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132017	.	terminator	4733	4773	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K132017	.	stop_codon	4766	4766	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132017	.	engineered_region	4733	4773	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132017	.	stem_loop	4740	4759	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132017	.	polyA_site	4760	4773	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132017	.	ribosome_entry_site	3805	3816	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K132017	.	conserved_region	3809	3812	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132017	.	CDS	3823	4611	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K132017	.	engineered_region	3823	4611	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K132017	.	start_codon	3823	3825	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K132017	.	engineered_tag	4612	4636	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K132017	.	stop_codon	4606	4611	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K132017	.	tag	4573	4605	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K132017	.	CDS	3823	4572	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K132017	.	engineered_region	670	3796	.	+	0	ID=BBa_K132007;Name=BBa_K132007;Parent=BBa_K132008
BBa_K132017	.	engineered_region	670	2918	.	+	0	ID=BBa_K132003;Name=BBa_K132003;Parent=BBa_K132007
BBa_K132017	.	engineered_region	1548	2918	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_K132003
BBa_K132017	.	ribosome_entry_site	1548	1559	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K132017	.	conserved_region	1552	1555	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132017	.	promoter	2864	2917	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K132017	.	promoter	2906	2911	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K132017	.	operator	2866	2882	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K132017	.	operator	2889	2905	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K132017	.	engineered_region	2864	2917	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K132017	.	promoter	2883	2888	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K132017	.	terminator	2727	2855	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K132017	.	terminator	2727	2806	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132017	.	stem_loop	2738	2781	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132017	.	engineered_region	2727	2806	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132017	.	terminator	2815	2855	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132017	.	stop_codon	2848	2848	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132017	.	engineered_region	2815	2855	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132017	.	stem_loop	2822	2841	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132017	.	polyA_site	2842	2855	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132017	.	CDS	1566	2693	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K132017	.	engineered_region	1566	2693	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K132017	.	engineered_tag	2694	2718	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K132017	.	CDS	1566	2693	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K132017	.	tag	2655	2693	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K132017	.	engineered_region	670	1539	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132003
BBa_K132017	.	tag	1501	1533	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132017	.	engineered_region	687	1500	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132017	.	ribosome_entry_site	670	681	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132017	.	stop_codon	1534	1539	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132017	.	sequence_feature	682	687	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132017	.	engineered_region	2927	3796	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132007
BBa_K132017	.	tag	3758	3790	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132017	.	engineered_region	2944	3757	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132017	.	ribosome_entry_site	2927	2938	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132017	.	stop_codon	3791	3796	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132017	.	sequence_feature	2939	2944	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132017	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K132017	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K132017	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K132017	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K132017	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K132017	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K132017	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K132017	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K132017
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagccatattcaacgggaaacg
tcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggc
aatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgt
tgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcatttt
atccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatc
ctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtcc
ttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgatttt
gatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcag
tcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacg
agtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaa
cggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttcg
ctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatggtgaatgtgaaa
ccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcc
acgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaaca
actggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcg
gcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgta
aagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccat
tgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatt
ttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgg
gcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgat
agcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccc
actgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtg
cggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga
ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctg
ttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgatt
cattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatacta
gagaaagaggagaaatactagatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatgg
atgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaa
gcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagacta
aactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcacca
ctgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggc
agtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcag
gcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaag
tctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataacct
tatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgcc
atcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctg
atatgaataaattgcagtttcatttgatgctcgatgagtttttcgctgcaaacgacgaaaactacgctttagtagctta
ataatactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttgg
atcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactga
agaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtc
gaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaag
accttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcat
tgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactac
aaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaat
cgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaa
tgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagag
aagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacg
acgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata