LOCUS BBa_K132019 5726 bp DNA linear 22-Nov-2024 DEFINITION rhlR+terminator+Prhl+luxI+kanR-LVA+lacI+PL+kanR-LVA+aiiA+terminator ACCESSION BBa_K132019 VERSION BBa_K132019.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..747 /label=BBa_C0171 stem_loop 767..810 /label=stem_loop misc_feature 756..835 /label=BBa_B0010 terminator 756..835 /label=BBa_B0010 stem_loop 851..870 /label=T7 TE misc_feature 877..877 /label=stop polyA_site 871..884 /label=polya misc_feature 844..884 /label=BBa_B0012 terminator 844..884 /label=BBa_B0012 misc_feature 893..912 /label=RhlR promoter 911..916 /label=-35 promoter 934..939 /label=-10 misc_feature 945..945 /label=start promoter 893..945 /label=BBa_R0071 misc_feature 1..945 /label=BBa_K132010 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 misc_feature 1551..1582 /label=LVA CDS 972..1550 /label=luxI misc_feature 972..1589 /label=BBa_C0061 misc_feature 1590..1614 /label=Help:Barcodes CDS 972..1589 /label=BBa_C0061 misc_feature 954..1614 /label=BBa_C0261 RBS 1623..1634 /label=RBS misc_feature 1635..1640 /label=scar misc_feature 1640..2453 /label=kanR gene misc_feature 2454..2486 /label=LVA misc_feature 2487..2492 /label=stop misc_feature 1623..2492 /label=BBa_K132000 misc_feature 2505..2508 /label=conserved RBS 2501..2512 /label=BBa_B0034 misc_feature 3608..3646 /label=LVA CDS 2519..3646 /label=lacI-LVA misc_feature 2519..3646 /label=BBa_C0012 misc_feature 3647..3671 /label=Help:Barcodes CDS 2519..3646 /label=BBa_C0012 stem_loop 3691..3734 /label=stem_loop misc_feature 3680..3759 /label=BBa_B0010 terminator 3680..3759 /label=BBa_B0010 stem_loop 3775..3794 /label=T7 TE misc_feature 3801..3801 /label=stop polyA_site 3795..3808 /label=polya misc_feature 3768..3808 /label=BBa_B0012 terminator 3768..3808 /label=BBa_B0012 terminator 3680..3808 /label=BBa_B0015 misc_feature 3819..3835 /label=lac O1 promoter 3836..3841 /label=-35 misc_feature 3842..3858 /label=lac O1 promoter 3859..3864 /label=-10 misc_feature 3817..3870 /label=BBa_R0011 promoter 3817..3870 /label=BBa_R0011 misc_feature 2501..3871 /label=BBa_Q04121 misc_feature 1623..3871 /label=BBa_K132003 RBS 3880..3891 /label=RBS misc_feature 3892..3897 /label=scar misc_feature 3897..4710 /label=kanR gene misc_feature 4711..4743 /label=LVA misc_feature 4744..4749 /label=stop misc_feature 3880..4749 /label=BBa_K132000 misc_feature 1623..4749 /label=BBa_K132007 misc_feature 4762..4765 /label=conserved RBS 4758..4769 /label=BBa_B0034 misc_feature 4776..4778 /label=start misc_feature 5559..5564 /label=2 misc_feature 5526..5558 /label=LVA CDS 4776..5525 /label=aiiA misc_feature 4776..5564 /label=BBa_C0060 misc_feature 5565..5589 /label=Help:Barcodes CDS 4776..5564 /label=BBa_C0060 stem_loop 5609..5652 /label=stem_loop misc_feature 5598..5677 /label=BBa_B0010 terminator 5598..5677 /label=BBa_B0010 stem_loop 5693..5712 /label=T7 TE misc_feature 5719..5719 /label=stop polyA_site 5713..5726 /label=polya misc_feature 5686..5726 /label=BBa_B0012 terminator 5686..5726 /label=BBa_B0012 misc_feature 4758..5726 /label=BBa_I0460 misc_feature 1623..5726 /label=BBa_K132008 misc_feature 954..5726 /label=BBa_K132017 ORIGIN 1 aaagaggaga aatactagat gaggaatgac ggaggctttt tgctgtggtg ggacggtttg 61 cgtagcgaga tgcagccgat ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa 121 gtgcggcgcc tgggcttcga ttactacgcc tatggcgtgc gccacacgat tcccttcacc 181 cggccgaaga ccgaggtcca tggcacctat cccaaggcct ggctggagcg ataccagatg 241 cagaactacg gggccgtgga tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg 301 gtctggagcg acagcctgtt cgaccagagc cggatgctct ggaacgaggc tcgcgattgg 361 ggcctctgtg tcggcgcgac cttgccgatc cgcgcgccga acaatttgct cagcgtgctt 421 tccgtggcgc gcgaccagca gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg 481 ctgcgttgca tgatcgagtt gctgacccag aagctgaccg acctggagca tccgatgctg 541 atgtccaacc cggtctgcct gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac 601 ggcaagagtt ccggggaaat cgccatcatc ctgagcatct ccgagagcac ggtgaacttc 661 caccacaaga acatccagaa gaagttcgac gcgccgaaca agacgctggc tgccgcctac 721 gccgcggcgc tgggtctcat ctaataatac tagagccagg catcaaataa aacgaaaggc 781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcctgtga 901 aatctggcag ttaccgttag ctttcgaatt ggctaaaaag tgttctacta gagaaagagg 961 agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg 1021 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact 1081 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt 1141 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg 1201 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc 1261 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact 1321 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag 1381 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta 1441 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg 1501 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg 1561 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 1621 agaaagagga gaaatactag atgagccata ttcaacggga aacgtcttgc tccaggccgc 1681 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 1741 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc 1801 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 1861 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 1921 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc 1981 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 2041 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 2101 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 2161 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg 2221 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 2281 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 2341 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 2401 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttcgctgcaa 2461 acgacgaaaa ctacgcttta gtagcttaat aatactagag aaagaggaga aatactagat 2521 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca 2581 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt 2641 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg 2701 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca 2761 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat 2821 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg 2881 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc 2941 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag 3001 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg 3061 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct 3121 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg 3181 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt 3241 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac 3301 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga 3361 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca 3421 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 3481 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 3541 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 3601 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta 3661 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3721 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3781 ccttcgggtg ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac 3841 attgtgagcg gataacaaga tactgagcac atactagaga aagaggagaa atactagatg 3901 agccatattc aacgggaaac gtcttgctcc aggccgcgat taaattccaa catggatgct 3961 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 4021 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 4081 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 4141 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 4201 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 4261 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 4321 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 4381 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 4441 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 4501 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 4561 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 4621 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 4681 cagtttcatt tgatgctcga tgagtttttc gctgcaaacg acgaaaacta cgctttagta 4741 gcttaataat actagagaaa gaggagaaat actagatgac agtaaagaag ctttatttcg 4801 tcccagcagg tcgttgtatg ttggatcatt cgtctgttaa tagtacatta acaccaggag 4861 aattattaga cttaccggtt tggtgttatc ttttggagac tgaagaagga cctattttag 4921 tagatacagg tatgccagaa agtgcagtta ataatgaagg tctttttaac ggtacatttg 4981 tcgaagggca ggttttaccg aaaatgactg aagaagatag aatcgtgaat attttaaaac 5041 gggttggtta tgagccggaa gaccttcttt atattattag ttctcacttg cattttgatc 5101 atgcaggagg aaatggcgct tttataaata caccaatcat tgtacagcgt gctgaatatg 5161 aggcggcgca gcatagcgaa gaatatttga aagaatgtat attgccgaat ttaaactaca 5221 aaatcattga aggtgattat gaagtcgtac caggagttca attattgcat acaccaggcc 5281 atactccagg gcatcaatcg ctattaattg agacagaaaa atccggtcct gtattattaa 5341 cgattgatgc atcgtatacg aaagagaatt ttgaaaatga agtgccattt gcgggatttg 5401 attcagaatt agctttatct tcaattaaac gtttaaaaga agtggtgatg aaagagaagc 5461 cgattgtttt ctttggacat gatatagagc aggaaagggg atgtaaagtg ttccctgaat 5521 atatagctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt 5581 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5641 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5701 ttcgggtggg cctttctgcg tttata //