##gff-version 3
##sequence-region BBa_K132019 1 5726
BBa_K132019	.	engineered_region	954	5726	.	+	0	ID=BBa_K132017;Name=BBa_K132017
BBa_K132019	.	engineered_region	954	1614	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K132017
BBa_K132019	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K132019	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132019	.	CDS	972	1589	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K132019	.	engineered_tag	1590	1614	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K132019	.	engineered_region	972	1589	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K132019	.	CDS	972	1550	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K132019	.	tag	1551	1582	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K132019	.	engineered_region	1623	5726	.	+	0	ID=BBa_K132008;Name=BBa_K132008;Parent=BBa_K132017
BBa_K132019	.	engineered_region	1623	4749	.	+	0	ID=BBa_K132007;Name=BBa_K132007;Parent=BBa_K132008
BBa_K132019	.	engineered_region	1623	3871	.	+	0	ID=BBa_K132003;Name=BBa_K132003;Parent=BBa_K132007
BBa_K132019	.	engineered_region	2501	3871	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_K132003
BBa_K132019	.	promoter	3817	3870	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K132019	.	operator	3819	3835	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K132019	.	engineered_region	3817	3870	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K132019	.	promoter	3836	3841	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K132019	.	operator	3842	3858	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K132019	.	promoter	3859	3864	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K132019	.	terminator	3680	3808	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K132019	.	terminator	3680	3759	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132019	.	stem_loop	3691	3734	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132019	.	engineered_region	3680	3759	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132019	.	terminator	3768	3808	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132019	.	polyA_site	3795	3808	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132019	.	stem_loop	3775	3794	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132019	.	engineered_region	3768	3808	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132019	.	stop_codon	3801	3801	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132019	.	CDS	2519	3646	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K132019	.	engineered_region	2519	3646	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K132019	.	CDS	2519	3646	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K132019	.	engineered_tag	3647	3671	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K132019	.	tag	3608	3646	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K132019	.	ribosome_entry_site	2501	2512	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K132019	.	conserved_region	2505	2508	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132019	.	engineered_region	1623	2492	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132003
BBa_K132019	.	engineered_region	1640	2453	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132019	.	stop_codon	2487	2492	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132019	.	tag	2454	2486	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132019	.	ribosome_entry_site	1623	1634	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132019	.	sequence_feature	1635	1640	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132019	.	engineered_region	3880	4749	.	+	0	ID=BBa_K132000;Name=BBa_K132000;Parent=BBa_K132007
BBa_K132019	.	engineered_region	3897	4710	.	+	0	ID=annotation1988217;Name=kanR gene;Parent=BBa_K132000
BBa_K132019	.	stop_codon	4744	4749	.	+	0	ID=annotation1988219;Name=stop;Parent=BBa_K132000
BBa_K132019	.	tag	4711	4743	.	+	0	ID=annotation1988218;Name=LVA;Parent=BBa_K132000
BBa_K132019	.	ribosome_entry_site	3880	3891	.	+	0	ID=annotation1987900;Name=RBS;Parent=BBa_K132000
BBa_K132019	.	sequence_feature	3892	3897	.	+	0	ID=annotation1988215;Name=scar;Parent=BBa_K132000
BBa_K132019	.	engineered_region	4758	5726	.	+	0	ID=BBa_I0460;Name=BBa_I0460;Parent=BBa_K132008
BBa_K132019	.	terminator	5598	5677	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K132019	.	stem_loop	5609	5652	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132019	.	engineered_region	5598	5677	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132019	.	CDS	4776	5564	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K132019	.	engineered_tag	5565	5589	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K132019	.	start_codon	4776	4778	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K132019	.	CDS	4776	5525	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K132019	.	tag	5526	5558	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K132019	.	stop_codon	5559	5564	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K132019	.	engineered_region	4776	5564	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K132019	.	terminator	5686	5726	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K132019	.	polyA_site	5713	5726	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132019	.	stem_loop	5693	5712	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132019	.	engineered_region	5686	5726	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132019	.	stop_codon	5719	5719	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132019	.	ribosome_entry_site	4758	4769	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K132019	.	conserved_region	4762	4765	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132019	.	engineered_region	1	945	.	+	0	ID=BBa_K132010;Name=BBa_K132010
BBa_K132019	.	terminator	756	835	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K132010
BBa_K132019	.	stem_loop	767	810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132019	.	engineered_region	756	835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132019	.	promoter	893	945	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K132010
BBa_K132019	.	promoter	911	916	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K132019	.	promoter	934	939	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K132019	.	non_covalent_binding_site	893	912	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K132019	.	start_codon	945	945	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K132019	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K132010
BBa_K132019	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132019	.	terminator	844	884	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K132010
BBa_K132019	.	polyA_site	871	884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132019	.	stem_loop	851	870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132019	.	engineered_region	844	884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132019	.	stop_codon	877	877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132019	.	CDS	19	747	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K132010
>BBa_K132019
aaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccga
tccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgt
gcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccag
atgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcc
tgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgc
gccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgc
ctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccgg
tctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcct
gagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggct
gccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctac
tagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataa
aggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatca
gatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttat
tacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaa
tatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaa
ctatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagc
gatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggt
tgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagta
gcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagccatattcaacggg
aaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgt
cgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggt
agcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagc
attttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaaga
atatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaat
tgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtg
attttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccgga
ttcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgtt
ggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattac
agaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtt
tttcgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatggtgaatg
tgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggc
cagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggca
caacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattg
tcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagc
ctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggat
gccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagta
ttattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgtt
agcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcag
ccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcg
ttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgt
tggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaa
caggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatc
agctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggc
cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgcttta
gtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
tactagagaaagaggagaaatactagatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaa
catggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtat
gggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtca
gactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttact
caccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcg
ctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcg
ctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttga
acaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgat
aaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatc
ttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataa
tcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttcgctgcaaacgacgaaaactacgctttagta
gcttaataatactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtat
gttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggag
actgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacat
ttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagcc
ggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacacca
atcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaa
actacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggca
tcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaatttt
gaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatga
aagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgc
aaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata