LOCUS       BBa_K132020             1957 bp    DNA     linear       21-Dec-2024
DEFINITION  araC+Pbad/araC+cmR
ACCESSION   BBa_K132020
VERSION     BBa_K132020.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..894
                     /label=araC
     misc_feature    895..927
                     /label=LVA
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             19..958
                     /label=BBa_C0080
     misc_feature    1..958
                     /label=BBa_S03519
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     terminator      967..1095
                     /label=BBa_B0015
     misc_feature    1..1095
                     /label=BBa_K132018
     promoter        1104..1233
                     /label=BBa_I13453
     RBS             1242..1253
                     /label=RBS
     misc_feature    1254..1261
                     /label=scar
     CDS             1261..1918
                     /label=cmR gene
     misc_feature    1918..1951
                     /label=LVA
     misc_feature    1951..1957
                     /label=stop
     misc_feature    1242..1957
                     /label=BBa_K132001
ORIGIN
        1 aaagaggaga aatactagat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg
       61 tttaacgccc atctggtggc gggtttaacg ccgattgagg ccaatggtta tctcgatttt
      121 tttatcgacc gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag
      181 ggggtggtga aaaatcaggg acgagaattt gtctgccgac cgggtgatat tttgctgttc
      241 ccgccaggag agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag
      301 tgggtttact ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt
      361 gccaatacgg gtttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt
      421 gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat
      481 ctgcttgagc aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg
      541 atggataatc gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat
      601 tttgatatcg ccagcgtcgc acagcatgtt tgcctgtcgc cgtcgcgtct gtcacatctt
      661 ttccgccagc agttagggat tagcgtctta agctggcgcg aggaccaacg catcagccag
      721 gcgaagctgc ttttgagcac tacccggatg cctatcgcca ccgtcggtcg caatgttggt
      781 tttgacgatc aactctattt ctcgcgagta tttaaaaaat gcaccggggc cagcccgagc
      841 gagttccgtg ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcagctgca
      901 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc
     1141 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     1201 tactgtttct ccataccgtt tttttgggct agctactaga gaaagaggag aaatactaga
     1261 gatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1321 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1381 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1441 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     1501 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1561 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1621 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1681 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1741 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1801 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1861 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcggc
     1921 tgcaaacgac gaaaactacg ctttagtagc ttaataa
//