LOCUS       BBa_K132021             1957 bp    DNA     linear       22-Nov-2024
DEFINITION  cmR-LVA+araC+terminator+Pbad/araC+cmR-LVA
ACCESSION   BBa_K132021
VERSION     BBa_K132021.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=RBS
     misc_feature    13..20
                     /label=scar
     CDS             20..677
                     /label=cmR gene
     misc_feature    677..710
                     /label=LVA
     misc_feature    710..716
                     /label=stop
     misc_feature    1..716
                     /label=BBa_K132001
     misc_feature    729..732
                     /label=conserved
     RBS             725..736
                     /label=BBa_B0034
     CDS             743..1618
                     /label=araC
     misc_feature    1619..1651
                     /label=LVA
     misc_feature    1658..1682
                     /label=Help:Barcodes
     CDS             743..1682
                     /label=BBa_C0080
     misc_feature    725..1682
                     /label=BBa_S03519
     stem_loop       1702..1745
                     /label=stem_loop
     misc_feature    1691..1770
                     /label=BBa_B0010
     terminator      1691..1770
                     /label=BBa_B0010
     stem_loop       1786..1805
                     /label=T7 TE
     misc_feature    1812..1812
                     /label=stop
     polyA_site      1806..1819
                     /label=polya
     misc_feature    1779..1819
                     /label=BBa_B0012
     terminator      1779..1819
                     /label=BBa_B0012
     terminator      1691..1819
                     /label=BBa_B0015
     misc_feature    725..1819
                     /label=BBa_K132018
     promoter        1828..1957
                     /label=BBa_I13453
ORIGIN
        1 aaagaggaga aatactagag atggagaaaa aaatcactgg atataccacc gttgatatat
       61 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      421 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      601 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
      661 agtggcaggg cggggcggct gcaaacgacg aaaactacgc tttagtagct taataatact
      721 agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc tgccgggata
      781 ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga
      841 tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg
      901 tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct
      961 gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca
     1021 ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat
     1081 atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct
     1141 gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat
     1201 aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc
     1261 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag
     1321 caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca
     1381 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag
     1441 ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt
     1501 tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc
     1561 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc
     1621 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
     1681 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1801 gggcctttct gcgtttatat actagagaca ttgattattt gcacggcgtc acactttgct
     1861 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
     1921 tctctactgt ttctccatac cgtttttttg ggctagc
//