##gff-version 3
##sequence-region BBa_K132021 1 1957
BBa_K132021	.	engineered_region	725	1819	.	+	0	ID=BBa_K132018;Name=BBa_K132018
BBa_K132021	.	engineered_region	725	1682	.	+	0	ID=BBa_S03519;Name=BBa_S03519;Parent=BBa_K132018
BBa_K132021	.	ribosome_entry_site	725	736	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03519
BBa_K132021	.	conserved_region	729	732	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132021	.	CDS	743	1682	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03519
BBa_K132021	.	engineered_tag	1658	1682	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K132021	.	tag	1619	1651	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K132021	.	CDS	743	1618	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K132021	.	terminator	1691	1819	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K132018
BBa_K132021	.	terminator	1691	1770	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132021	.	stem_loop	1702	1745	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132021	.	engineered_region	1691	1770	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132021	.	terminator	1779	1819	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132021	.	polyA_site	1806	1819	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132021	.	stem_loop	1786	1805	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132021	.	engineered_region	1779	1819	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132021	.	stop_codon	1812	1812	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132021	.	promoter	1828	1957	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K132021	.	engineered_region	1	716	.	+	0	ID=BBa_K132001;Name=BBa_K132001
BBa_K132021	.	ribosome_entry_site	1	12	.	+	0	ID=annotation1988057;Name=RBS;Parent=BBa_K132001
BBa_K132021	.	stop_codon	710	716	.	+	0	ID=annotation1988214;Name=stop;Parent=BBa_K132001
BBa_K132021	.	tag	677	710	.	+	0	ID=annotation1988213;Name=LVA;Parent=BBa_K132001
BBa_K132021	.	sequence_feature	13	20	.	+	0	ID=annotation1988058;Name=scar;Parent=BBa_K132001
BBa_K132021	.	CDS	20	677	.	+	0	ID=annotation1988212;Name=cmR gene;Parent=BBa_K132001
>BBa_K132021
aaagaggagaaatactagagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaaga
acattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaag
accgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaat
ttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaac
tgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcg
tgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtga
gtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatac
gcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatg
cttaatgaattacaacagtactgcgatgagtggcagggcggggcggctgcaaacgacgaaaactacgctttagtagctt
aataatactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaac
gcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatga
aaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccggg
tgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgg
gtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcc
cggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattc
ggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccg
atggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcg
cacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcg
cgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgtt
ggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggtt
gtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatc
ctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc