LOCUS BBa_K132023 4351 bp DNA linear 22-Nov-2024 DEFINITION rhlI+cmR-LVA+araC+terminator+Pbad/araC+cmR-LVA+aiiA+terminator ACCESSION BBa_K132023 VERSION BBa_K132023.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..621 /label=rhlL misc_feature 622..654 /label=LVA misc_feature 661..685 /label=Help:Barcodes CDS 19..660 /label=BBa_C0070 misc_feature 1..685 /label=BBa_I9026 RBS 694..705 /label=RBS misc_feature 706..713 /label=scar CDS 713..1370 /label=cmR gene misc_feature 1370..1403 /label=LVA misc_feature 1403..1409 /label=stop misc_feature 694..1409 /label=BBa_K132001 misc_feature 1422..1425 /label=conserved RBS 1418..1429 /label=BBa_B0034 CDS 1436..2311 /label=araC misc_feature 2312..2344 /label=LVA misc_feature 2351..2375 /label=Help:Barcodes CDS 1436..2375 /label=BBa_C0080 misc_feature 1418..2375 /label=BBa_S03519 stem_loop 2395..2438 /label=stem_loop misc_feature 2384..2463 /label=BBa_B0010 terminator 2384..2463 /label=BBa_B0010 stem_loop 2479..2498 /label=T7 TE misc_feature 2505..2505 /label=stop polyA_site 2499..2512 /label=polya misc_feature 2472..2512 /label=BBa_B0012 terminator 2472..2512 /label=BBa_B0012 terminator 2384..2512 /label=BBa_B0015 misc_feature 1418..2512 /label=BBa_K132018 promoter 2521..2650 /label=BBa_I13453 RBS 2659..2670 /label=RBS misc_feature 2671..2678 /label=scar CDS 2678..3335 /label=cmR gene misc_feature 3335..3368 /label=LVA misc_feature 3368..3374 /label=stop misc_feature 2659..3374 /label=BBa_K132001 misc_feature 1418..3374 /label=BBa_K132020 misc_feature 3387..3390 /label=conserved RBS 3383..3394 /label=BBa_B0034 misc_feature 3401..3403 /label=start misc_feature 4184..4189 /label=2 misc_feature 4151..4183 /label=LVA CDS 3401..4150 /label=aiiA misc_feature 3401..4189 /label=BBa_C0060 misc_feature 4190..4214 /label=Help:Barcodes CDS 3401..4189 /label=BBa_C0060 stem_loop 4234..4277 /label=stem_loop misc_feature 4223..4302 /label=BBa_B0010 terminator 4223..4302 /label=BBa_B0010 stem_loop 4318..4337 /label=T7 TE misc_feature 4344..4344 /label=stop polyA_site 4338..4351 /label=polya misc_feature 4311..4351 /label=BBa_B0012 terminator 4311..4351 /label=BBa_B0012 misc_feature 3383..4351 /label=BBa_I0460 misc_feature 694..4351 /label=BBa_K132012 ORIGIN 1 aaagaggaga aatactagat gatcgaactg ctgtccgaat ccctggaagg tctgtccgct 61 gctatgatcg ctgaactggg tcgttaccgt caccaggttt tcatcgaaaa actgggttgg 121 gacgttgttt ccacctcccg tgttcgtgac caggagttcg accagttcga ccacccgcag 181 acccgttaca tcgttgctat gtcccgtcag ggtatctgcg gttgcgctcg tctgctgccg 241 accaccgacg cttacctgct gaaagacgtt ttcgcttacc tgtgctccga aaccccgccg 301 tccgacccgt ccgtttggga actgtcccgt tacgctgctt ccgctgctga cgacccgcag 361 ctggctatga aaatcttctg gtcctccctc cagtgcgctt ggtacctggg tgcttcctcc 421 gttgttgctg ttaccaccac cgctatggaa cgttacttcg ttcgtaacgg tgttatcctc 481 cagcgtctgg gtccgccgca gaaagttaaa ggtgaaaccc tggttgctat ctccttcccg 541 gcttaccagg aacgtggtct ggaaatgctg ctgcgttacc acccggaatg gctccagggt 601 gttccgctgt ccatggctgt tgctgcaaac gacgaaaact acgctttagt agcttaataa 661 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gagatggaga 721 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 781 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 841 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 901 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg 961 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 1021 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 1081 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 1141 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 1201 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 1261 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 1321 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg gctgcaaacg 1381 acgaaaacta cgctttagta gcttaataat actagagaaa gaggagaaat actagatggc 1441 tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc tggtggcggg 1501 tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac cgctgggaat 1561 gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg 1621 agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta 1681 cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc 1741 ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt tctttcgccc 1801 ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca 1861 aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg 1921 gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc 1981 ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca 2041 gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag 2101 cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt tgagcactac 2161 ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc 2221 gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga 2281 aaaagtgaat gatgtagccg tcaagttgtc agctgcaaac gacgaaaact acgctttagt 2341 agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 2401 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 2461 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 2521 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 2581 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 2641 ttgggctagc tactagagaa agaggagaaa tactagagat ggagaaaaaa atcactggat 2701 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 2761 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 2821 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 2881 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg 2941 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 3001 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 3061 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 3121 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 3181 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 3241 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat 3301 tacaacagta ctgcgatgag tggcagggcg gggcggctgc aaacgacgaa aactacgctt 3361 tagtagctta ataatactag agaaagagga gaaatactag atgacagtaa agaagcttta 3421 tttcgtccca gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc 3481 aggagaatta ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat 3541 tttagtagat acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac 3601 atttgtcgaa gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt 3661 aaaacgggtt ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt 3721 tgatcatgca ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga 3781 atatgaggcg gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa 3841 ctacaaaatc attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc 3901 aggccatact ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt 3961 attaacgatt gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg 4021 atttgattca gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga 4081 gaagccgatt gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc 4141 tgaatatata gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 4201 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 4261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 4321 tcaccttcgg gtgggccttt ctgcgtttat a //