##gff-version 3
##sequence-region BBa_K132023 1 4351
BBa_K132023	.	engineered_region	1	685	.	+	0	ID=BBa_I9026;Name=BBa_I9026
BBa_K132023	.	CDS	19	660	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I9026
BBa_K132023	.	CDS	19	621	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K132023	.	engineered_tag	661	685	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K132023	.	tag	622	654	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K132023	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I9026
BBa_K132023	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132023	.	engineered_region	694	4351	.	+	0	ID=BBa_K132012;Name=BBa_K132012
BBa_K132023	.	engineered_region	694	1409	.	+	0	ID=BBa_K132001;Name=BBa_K132001;Parent=BBa_K132012
BBa_K132023	.	ribosome_entry_site	694	705	.	+	0	ID=annotation1988057;Name=RBS;Parent=BBa_K132001
BBa_K132023	.	stop_codon	1403	1409	.	+	0	ID=annotation1988214;Name=stop;Parent=BBa_K132001
BBa_K132023	.	tag	1370	1403	.	+	0	ID=annotation1988213;Name=LVA;Parent=BBa_K132001
BBa_K132023	.	sequence_feature	706	713	.	+	0	ID=annotation1988058;Name=scar;Parent=BBa_K132001
BBa_K132023	.	CDS	713	1370	.	+	0	ID=annotation1988212;Name=cmR gene;Parent=BBa_K132001
BBa_K132023	.	engineered_region	1418	3374	.	+	0	ID=BBa_K132020;Name=BBa_K132020;Parent=BBa_K132012
BBa_K132023	.	engineered_region	1418	2512	.	+	0	ID=BBa_K132018;Name=BBa_K132018;Parent=BBa_K132020
BBa_K132023	.	engineered_region	1418	2375	.	+	0	ID=BBa_S03519;Name=BBa_S03519;Parent=BBa_K132018
BBa_K132023	.	ribosome_entry_site	1418	1429	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03519
BBa_K132023	.	conserved_region	1422	1425	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132023	.	CDS	1436	2375	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03519
BBa_K132023	.	engineered_tag	2351	2375	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K132023	.	tag	2312	2344	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K132023	.	CDS	1436	2311	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K132023	.	terminator	2384	2512	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K132018
BBa_K132023	.	terminator	2384	2463	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132023	.	stem_loop	2395	2438	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132023	.	engineered_region	2384	2463	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132023	.	terminator	2472	2512	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132023	.	polyA_site	2499	2512	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132023	.	stem_loop	2479	2498	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132023	.	engineered_region	2472	2512	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132023	.	stop_codon	2505	2505	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132023	.	promoter	2521	2650	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K132020
BBa_K132023	.	engineered_region	2659	3374	.	+	0	ID=BBa_K132001;Name=BBa_K132001;Parent=BBa_K132020
BBa_K132023	.	ribosome_entry_site	2659	2670	.	+	0	ID=annotation1988057;Name=RBS;Parent=BBa_K132001
BBa_K132023	.	stop_codon	3368	3374	.	+	0	ID=annotation1988214;Name=stop;Parent=BBa_K132001
BBa_K132023	.	tag	3335	3368	.	+	0	ID=annotation1988213;Name=LVA;Parent=BBa_K132001
BBa_K132023	.	sequence_feature	2671	2678	.	+	0	ID=annotation1988058;Name=scar;Parent=BBa_K132001
BBa_K132023	.	CDS	2678	3335	.	+	0	ID=annotation1988212;Name=cmR gene;Parent=BBa_K132001
BBa_K132023	.	engineered_region	3383	4351	.	+	0	ID=BBa_I0460;Name=BBa_I0460;Parent=BBa_K132012
BBa_K132023	.	terminator	4223	4302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K132023	.	stem_loop	4234	4277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132023	.	engineered_region	4223	4302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132023	.	CDS	3401	4189	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K132023	.	engineered_tag	4190	4214	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K132023	.	start_codon	3401	3403	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K132023	.	CDS	3401	4150	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K132023	.	tag	4151	4183	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K132023	.	stop_codon	4184	4189	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K132023	.	engineered_region	3401	4189	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K132023	.	terminator	4311	4351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K132023	.	polyA_site	4338	4351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132023	.	stem_loop	4318	4337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132023	.	engineered_region	4311	4351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132023	.	stop_codon	4344	4344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132023	.	ribosome_entry_site	3383	3394	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K132023	.	conserved_region	3387	3390	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K132023
aaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgg
gtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagtt
cgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctg
ccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgttt
gggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtg
cgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgtt
atcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaac
gtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactag
agatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttca
gtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataag
cacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaag
acggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgct
ctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctg
gcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatt
taaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgct
gatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacag
tactgcgatgagtggcagggcggggcggctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaaga
ggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggt
ttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatc
tcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttccc
gccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgc
gcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagc
cgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaa
tctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgc
gaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgt
cgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcag
ccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactc
tatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatg
atgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtag
atcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgat
tatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttat
cgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagagatggagaa
aaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgct
caatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagtttt
atccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagct
ggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaa
taccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttcc
ctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggc
caatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctg
gcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatg
agtggcagggcggggcggctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatac
tagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaa
caccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacagg
tatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgact
gaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcact
tgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggc
gcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtc
gtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccg
gtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattc
agaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgat
atagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata