LOCUS BBa_K132024 5420 bp DNA linear 22-Dec-2024 DEFINITION Ptet+luxR+Plux+rhlI+cmR-LVA+araC+terminator+Pbad/araC+cmR-LVA+aiiA+ terminator ACCESSION BBa_K132024 VERSION BBa_K132024.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1690 /label=rhlL misc_feature 1691..1723 /label=LVA misc_feature 1730..1754 /label=Help:Barcodes CDS 1088..1729 /label=BBa_C0070 misc_feature 1070..1754 /label=BBa_I9026 RBS 1763..1774 /label=RBS misc_feature 1775..1782 /label=scar CDS 1782..2439 /label=cmR gene misc_feature 2439..2472 /label=LVA misc_feature 2472..2478 /label=stop misc_feature 1763..2478 /label=BBa_K132001 misc_feature 2491..2494 /label=conserved RBS 2487..2498 /label=BBa_B0034 CDS 2505..3380 /label=araC misc_feature 3381..3413 /label=LVA misc_feature 3420..3444 /label=Help:Barcodes CDS 2505..3444 /label=BBa_C0080 misc_feature 2487..3444 /label=BBa_S03519 stem_loop 3464..3507 /label=stem_loop misc_feature 3453..3532 /label=BBa_B0010 terminator 3453..3532 /label=BBa_B0010 stem_loop 3548..3567 /label=T7 TE misc_feature 3574..3574 /label=stop polyA_site 3568..3581 /label=polya misc_feature 3541..3581 /label=BBa_B0012 terminator 3541..3581 /label=BBa_B0012 terminator 3453..3581 /label=BBa_B0015 misc_feature 2487..3581 /label=BBa_K132018 promoter 3590..3719 /label=BBa_I13453 RBS 3728..3739 /label=RBS misc_feature 3740..3747 /label=scar CDS 3747..4404 /label=cmR gene misc_feature 4404..4437 /label=LVA misc_feature 4437..4443 /label=stop misc_feature 3728..4443 /label=BBa_K132001 misc_feature 2487..4443 /label=BBa_K132020 misc_feature 4456..4459 /label=conserved RBS 4452..4463 /label=BBa_B0034 misc_feature 4470..4472 /label=start misc_feature 5253..5258 /label=2 misc_feature 5220..5252 /label=LVA CDS 4470..5219 /label=aiiA misc_feature 4470..5258 /label=BBa_C0060 misc_feature 5259..5283 /label=Help:Barcodes CDS 4470..5258 /label=BBa_C0060 stem_loop 5303..5346 /label=stem_loop misc_feature 5292..5371 /label=BBa_B0010 terminator 5292..5371 /label=BBa_B0010 stem_loop 5387..5406 /label=T7 TE misc_feature 5413..5413 /label=stop polyA_site 5407..5420 /label=polya misc_feature 5380..5420 /label=BBa_B0012 terminator 5380..5420 /label=BBa_B0012 misc_feature 4452..5420 /label=BBa_I0460 misc_feature 1763..5420 /label=BBa_K132012 misc_feature 1070..5420 /label=BBa_K132023 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg atcgaactgc tgtccgaatc cctggaaggt ctgtccgctg ctatgatcgc 1141 tgaactgggt cgttaccgtc accaggtttt catcgaaaaa ctgggttggg acgttgtttc 1201 cacctcccgt gttcgtgacc aggagttcga ccagttcgac cacccgcaga cccgttacat 1261 cgttgctatg tcccgtcagg gtatctgcgg ttgcgctcgt ctgctgccga ccaccgacgc 1321 ttacctgctg aaagacgttt tcgcttacct gtgctccgaa accccgccgt ccgacccgtc 1381 cgtttgggaa ctgtcccgtt acgctgcttc cgctgctgac gacccgcagc tggctatgaa 1441 aatcttctgg tcctccctcc agtgcgcttg gtacctgggt gcttcctccg ttgttgctgt 1501 taccaccacc gctatggaac gttacttcgt tcgtaacggt gttatcctcc agcgtctggg 1561 tccgccgcag aaagttaaag gtgaaaccct ggttgctatc tccttcccgg cttaccagga 1621 acgtggtctg gaaatgctgc tgcgttacca cccggaatgg ctccagggtg ttccgctgtc 1681 catggctgtt gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1741 ctagtgtaga tctctactag agaaagagga gaaatactag agatggagaa aaaaatcact 1801 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 1861 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 1921 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 1981 atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat 2041 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 2101 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 2161 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 2221 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 2281 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 2341 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat 2401 gaattacaac agtactgcga tgagtggcag ggcggggcgg ctgcaaacga cgaaaactac 2461 gctttagtag cttaataata ctagagaaag aggagaaata ctagatggct gaagcgcaaa 2521 atgatcccct gctgccggga tactcgttta acgcccatct ggtggcgggt ttaacgccga 2581 ttgaggccaa tggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata 2641 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgtct 2701 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc 2761 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg 2821 aatggcttaa ctggccgtca atatttgcca atacgggttt ctttcgcccg gatgaagcgc 2881 accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc 2941 gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag 3001 cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca 3061 tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgcc 3121 tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct 3181 ggcgcgagga ccaacgcatc agccaggcga agctgctttt gagcactacc cggatgccta 3241 tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg cgagtattta 3301 aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg 3361 atgtagccgt caagttgtca gctgcaaacg acgaaaacta cgctttagta gcttaataac 3421 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 3481 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3541 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga cattgattat 3601 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct 3661 acctgacgct ttttatcgca actctctact gtttctccat accgtttttt tgggctagct 3721 actagagaaa gaggagaaat actagagatg gagaaaaaaa tcactggata taccaccgtt 3781 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 3841 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 3901 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 3961 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 4021 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 4081 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 4141 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 4201 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 4261 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 4321 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 4381 tgcgatgagt ggcagggcgg ggcggctgca aacgacgaaa actacgcttt agtagcttaa 4441 taatactaga gaaagaggag aaatactaga tgacagtaaa gaagctttat ttcgtcccag 4501 caggtcgttg tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat 4561 tagacttacc ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata 4621 caggtatgcc agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag 4681 ggcaggtttt accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg 4741 gttatgagcc ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag 4801 gaggaaatgg cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg 4861 cgcagcatag cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca 4921 ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc 4981 cagggcatca atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg 5041 atgcatcgta tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag 5101 aattagcttt atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg 5161 ttttctttgg acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag 5221 ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat 5281 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5341 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5401 tgggcctttc tgcgtttata //