##gff-version 3
##sequence-region BBa_K132024 1 5420
BBa_K132024	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_K132024	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_K132024	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132024	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_K132024	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K132024	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K132024	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K132024	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K132024	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K132024	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_K132024	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132024	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132024	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132024	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132024	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132024	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132024	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132024	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132024	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_K132024	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K132024	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K132024	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K132024	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K132024	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K132024	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K132024	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_K132024	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K132024	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K132024	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K132024	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K132024	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K132024	.	engineered_region	1070	5420	.	+	0	ID=BBa_K132023;Name=BBa_K132023
BBa_K132024	.	engineered_region	1070	1754	.	+	0	ID=BBa_I9026;Name=BBa_I9026;Parent=BBa_K132023
BBa_K132024	.	CDS	1088	1729	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I9026
BBa_K132024	.	engineered_tag	1730	1754	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K132024	.	CDS	1088	1690	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K132024	.	tag	1691	1723	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K132024	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I9026
BBa_K132024	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132024	.	engineered_region	1763	5420	.	+	0	ID=BBa_K132012;Name=BBa_K132012;Parent=BBa_K132023
BBa_K132024	.	engineered_region	2487	4443	.	+	0	ID=BBa_K132020;Name=BBa_K132020;Parent=BBa_K132012
BBa_K132024	.	engineered_region	3728	4443	.	+	0	ID=BBa_K132001;Name=BBa_K132001;Parent=BBa_K132020
BBa_K132024	.	sequence_feature	3740	3747	.	+	0	ID=annotation1988058;Name=scar;Parent=BBa_K132001
BBa_K132024	.	ribosome_entry_site	3728	3739	.	+	0	ID=annotation1988057;Name=RBS;Parent=BBa_K132001
BBa_K132024	.	tag	4404	4437	.	+	0	ID=annotation1988213;Name=LVA;Parent=BBa_K132001
BBa_K132024	.	stop_codon	4437	4443	.	+	0	ID=annotation1988214;Name=stop;Parent=BBa_K132001
BBa_K132024	.	CDS	3747	4404	.	+	0	ID=annotation1988212;Name=cmR gene;Parent=BBa_K132001
BBa_K132024	.	promoter	3590	3719	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K132020
BBa_K132024	.	engineered_region	2487	3581	.	+	0	ID=BBa_K132018;Name=BBa_K132018;Parent=BBa_K132020
BBa_K132024	.	terminator	3453	3581	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K132018
BBa_K132024	.	terminator	3541	3581	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K132024	.	stem_loop	3548	3567	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132024	.	engineered_region	3541	3581	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132024	.	stop_codon	3574	3574	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132024	.	polyA_site	3568	3581	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132024	.	terminator	3453	3532	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K132024	.	engineered_region	3453	3532	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132024	.	stem_loop	3464	3507	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132024	.	engineered_region	2487	3444	.	+	0	ID=BBa_S03519;Name=BBa_S03519;Parent=BBa_K132018
BBa_K132024	.	CDS	2505	3444	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03519
BBa_K132024	.	engineered_tag	3420	3444	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K132024	.	CDS	2505	3380	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K132024	.	tag	3381	3413	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K132024	.	ribosome_entry_site	2487	2498	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03519
BBa_K132024	.	conserved_region	2491	2494	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132024	.	engineered_region	4452	5420	.	+	0	ID=BBa_I0460;Name=BBa_I0460;Parent=BBa_K132012
BBa_K132024	.	terminator	5292	5371	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_K132024	.	engineered_region	5292	5371	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K132024	.	stem_loop	5303	5346	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K132024	.	ribosome_entry_site	4452	4463	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_K132024	.	conserved_region	4456	4459	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K132024	.	CDS	4470	5258	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_K132024	.	stop_codon	5253	5258	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K132024	.	CDS	4470	5219	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K132024	.	engineered_region	4470	5258	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K132024	.	engineered_tag	5259	5283	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K132024	.	tag	5220	5252	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K132024	.	start_codon	4470	4472	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K132024	.	terminator	5380	5420	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_K132024	.	stem_loop	5387	5406	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K132024	.	engineered_region	5380	5420	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K132024	.	stop_codon	5413	5413	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K132024	.	polyA_site	5407	5420	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K132024	.	engineered_region	1763	2478	.	+	0	ID=BBa_K132001;Name=BBa_K132001;Parent=BBa_K132012
BBa_K132024	.	sequence_feature	1775	1782	.	+	0	ID=annotation1988058;Name=scar;Parent=BBa_K132001
BBa_K132024	.	ribosome_entry_site	1763	1774	.	+	0	ID=annotation1988057;Name=RBS;Parent=BBa_K132001
BBa_K132024	.	tag	2439	2472	.	+	0	ID=annotation1988213;Name=LVA;Parent=BBa_K132001
BBa_K132024	.	stop_codon	2472	2478	.	+	0	ID=annotation1988214;Name=stop;Parent=BBa_K132001
BBa_K132024	.	CDS	1782	2439	.	+	0	ID=annotation1988212;Name=cmR gene;Parent=BBa_K132001
>BBa_K132024
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgatcgaactgctgtccg
aatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactggg
ttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgtt
gctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcg
cttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacga
cccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgtt
accaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaag
gtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatg
gctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagaaagaggagaaatactagagatggagaaaaaaatcactggatataccaccgttga
tatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcag
ctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgccc
gcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttg
ttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttcta
cacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttt
tcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgt
tttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgt
gatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcggctgcaaacg
acgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatggctgaagcgcaaaatgatccc
ctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgattttt
ttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcaggg
acgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggag
gctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatat
ttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgc
cgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcg
attaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagaca
gcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagtt
agggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcct
atcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggcca
gcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaa
ctacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcat
ttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgg
gctagctactagagaaagaggagaaatactagagatggagaaaaaaatcactggatataccaccgttgatatatcccaa
tggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatatta
cggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaa
tgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgtt
ttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatatt
cgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagc
caatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatg
ggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttcc
atgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcggctgcaaacgacgaaaacta
cgctttagtagcttaataatactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcag
gtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgtta
tcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtcttttt
aacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttg
gttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttat
aaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattg
ccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccata
ctccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaa
agagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaa
gtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaat
atatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata