LOCUS BBa_K132025 6511 bp DNA linear 30-Dec-2025 DEFINITION Plac+rfp+Ptet+luxR+Plux+rhlI+cmR-LVA+araC+terminator+Pbad/araC+cmR- LVA+aiiA+terminator ACCESSION BBa_K132025 VERSION BBa_K132025.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..901 /label=mrfp1 misc_feature 941..946 /label=Stop misc_feature 902..940 /label=LVA misc_feature 227..229 /label=Start misc_feature 227..946 /label=BBa_J04051 stem_loop 966..1009 /label=stem_loop misc_feature 955..1034 /label=BBa_B0010 terminator 955..1034 /label=BBa_B0010 stem_loop 1050..1069 /label=T7 TE misc_feature 1076..1076 /label=stop polyA_site 1070..1083 /label=polya misc_feature 1043..1083 /label=BBa_B0012 terminator 1043..1083 /label=BBa_B0012 misc_feature 1..1083 /label=BBa_J04451 misc_feature 1092..1110 /label=TetR 1 misc_feature 1092..1145 /label=BBa_R0040 promoter 1111..1116 /label=-35 misc_feature 1117..1135 /label=TetR 2 promoter 1134..1139 /label=-10 promoter 1092..1145 /label=BBa_R0040 misc_feature 1158..1161 /label=conserved RBS 1154..1165 /label=BBa_B0034 misc_feature 1172..1173 /label=prefix misc_feature 1345..1345 /label=T misc_feature 1663..1663 /label=A CDS 1172..1921 /label=luxR misc_feature 1172..1927 /label=BBa_C0062 misc_feature 1928..1952 /label=Help:Barcodes CDS 1172..1927 /label=BBa_C0062 stem_loop 1972..2015 /label=stem_loop misc_feature 1961..2040 /label=BBa_B0010 terminator 1961..2040 /label=BBa_B0010 stem_loop 2056..2075 /label=T7 TE misc_feature 2082..2082 /label=stop polyA_site 2076..2089 /label=polya misc_feature 2049..2089 /label=BBa_B0012 terminator 2049..2089 /label=BBa_B0012 terminator 1961..2089 /label=BBa_B0015 misc_feature 2098..2117 /label=LuxR/HSL promoter 2117..2122 /label=-35 promoter 2139..2144 /label=-10 misc_feature 2150..2150 /label=start misc_feature 2098..2152 /label=BBa_R0062 promoter 2098..2152 /label=BBa_R0062 misc_feature 1092..2152 /label=BBa_F2620 misc_feature 2165..2168 /label=conserved RBS 2161..2172 /label=BBa_B0034 CDS 2179..2781 /label=rhlL misc_feature 2782..2814 /label=LVA misc_feature 2821..2845 /label=Help:Barcodes CDS 2179..2820 /label=BBa_C0070 misc_feature 2161..2845 /label=BBa_I9026 RBS 2854..2865 /label=RBS misc_feature 2866..2873 /label=scar CDS 2873..3530 /label=cmR gene misc_feature 3530..3563 /label=LVA misc_feature 3563..3569 /label=stop misc_feature 2854..3569 /label=BBa_K132001 misc_feature 3582..3585 /label=conserved RBS 3578..3589 /label=BBa_B0034 CDS 3596..4471 /label=araC misc_feature 4472..4504 /label=LVA misc_feature 4511..4535 /label=Help:Barcodes CDS 3596..4535 /label=BBa_C0080 misc_feature 3578..4535 /label=BBa_S03519 stem_loop 4555..4598 /label=stem_loop misc_feature 4544..4623 /label=BBa_B0010 terminator 4544..4623 /label=BBa_B0010 stem_loop 4639..4658 /label=T7 TE misc_feature 4665..4665 /label=stop polyA_site 4659..4672 /label=polya misc_feature 4632..4672 /label=BBa_B0012 terminator 4632..4672 /label=BBa_B0012 terminator 4544..4672 /label=BBa_B0015 misc_feature 3578..4672 /label=BBa_K132018 promoter 4681..4810 /label=BBa_I13453 RBS 4819..4830 /label=RBS misc_feature 4831..4838 /label=scar CDS 4838..5495 /label=cmR gene misc_feature 5495..5528 /label=LVA misc_feature 5528..5534 /label=stop misc_feature 4819..5534 /label=BBa_K132001 misc_feature 3578..5534 /label=BBa_K132020 misc_feature 5547..5550 /label=conserved RBS 5543..5554 /label=BBa_B0034 misc_feature 5561..5563 /label=start misc_feature 6344..6349 /label=2 misc_feature 6311..6343 /label=LVA CDS 5561..6310 /label=aiiA misc_feature 5561..6349 /label=BBa_C0060 misc_feature 6350..6374 /label=Help:Barcodes CDS 5561..6349 /label=BBa_C0060 stem_loop 6394..6437 /label=stem_loop misc_feature 6383..6462 /label=BBa_B0010 terminator 6383..6462 /label=BBa_B0010 stem_loop 6478..6497 /label=T7 TE misc_feature 6504..6504 /label=stop polyA_site 6498..6511 /label=polya misc_feature 6471..6511 /label=BBa_B0012 terminator 6471..6511 /label=BBa_B0012 misc_feature 5543..6511 /label=BBa_I0460 misc_feature 2854..6511 /label=BBa_K132012 misc_feature 2161..6511 /label=BBa_K132023 misc_feature 1092..6511 /label=BBa_K132024 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga 241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 901 taggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc 961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 1081 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 1141 agcactacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 1201 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 1261 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 1321 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 1381 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 1441 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 1501 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 1561 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 1621 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 1681 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 1741 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 1801 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 1861 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 1921 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 1981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagacc 2101 tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag 2161 aaagaggaga aatactagat gatcgaactg ctgtccgaat ccctggaagg tctgtccgct 2221 gctatgatcg ctgaactggg tcgttaccgt caccaggttt tcatcgaaaa actgggttgg 2281 gacgttgttt ccacctcccg tgttcgtgac caggagttcg accagttcga ccacccgcag 2341 acccgttaca tcgttgctat gtcccgtcag ggtatctgcg gttgcgctcg tctgctgccg 2401 accaccgacg cttacctgct gaaagacgtt ttcgcttacc tgtgctccga aaccccgccg 2461 tccgacccgt ccgtttggga actgtcccgt tacgctgctt ccgctgctga cgacccgcag 2521 ctggctatga aaatcttctg gtcctccctc cagtgcgctt ggtacctggg tgcttcctcc 2581 gttgttgctg ttaccaccac cgctatggaa cgttacttcg ttcgtaacgg tgttatcctc 2641 cagcgtctgg gtccgccgca gaaagttaaa ggtgaaaccc tggttgctat ctccttcccg 2701 gcttaccagg aacgtggtct ggaaatgctg ctgcgttacc acccggaatg gctccagggt 2761 gttccgctgt ccatggctgt tgctgcaaac gacgaaaact acgctttagt agcttaataa 2821 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gagatggaga 2881 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 2941 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 3001 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 3061 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg 3121 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 3181 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 3241 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 3301 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 3361 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 3421 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 3481 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg gctgcaaacg 3541 acgaaaacta cgctttagta gcttaataat actagagaaa gaggagaaat actagatggc 3601 tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc tggtggcggg 3661 tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac cgctgggaat 3721 gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg 3781 agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta 3841 cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc 3901 ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt tctttcgccc 3961 ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca 4021 aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg 4081 gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc 4141 ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca 4201 gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag 4261 cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt tgagcactac 4321 ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc 4381 gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga 4441 aaaagtgaat gatgtagccg tcaagttgtc agctgcaaac gacgaaaact acgctttagt 4501 agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 4561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 4621 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 4681 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 4741 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 4801 ttgggctagc tactagagaa agaggagaaa tactagagat ggagaaaaaa atcactggat 4861 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 4921 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 4981 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 5041 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg 5101 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 5161 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 5221 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 5281 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 5341 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 5401 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat 5461 tacaacagta ctgcgatgag tggcagggcg gggcggctgc aaacgacgaa aactacgctt 5521 tagtagctta ataatactag agaaagagga gaaatactag atgacagtaa agaagcttta 5581 tttcgtccca gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc 5641 aggagaatta ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat 5701 tttagtagat acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac 5761 atttgtcgaa gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt 5821 aaaacgggtt ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt 5881 tgatcatgca ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga 5941 atatgaggcg gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa 6001 ctacaaaatc attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc 6061 aggccatact ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt 6121 attaacgatt gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg 6181 atttgattca gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga 6241 gaagccgatt gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc 6301 tgaatatata gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 6361 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 6421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 6481 tcaccttcgg gtgggccttt ctgcgtttat a //