BBa_K1321005
1
BBa_K1321005
Synthetic Phytochelatin EC(20) RFC[25]
2014-10-06T11:00:00Z
2015-05-08T01:09:51Z
Sequence is not natural. Basis for the sequence was from the following reference: Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.
Synthetic Phytochelatin EC(20) rfc25 fusion format
false
false
_1696_
0
20780
9
In stock
true
Codon optimised for E.coli.
false
Xenia Spencer-Milnes
annotation2414598
1
Synthetic Phytochelatin EC(20)
range2414598
1
1
123
BBa_K1321014
1
BBa_K1321014
CBDCipA with N and C-terminal linker
2014-10-14T11:00:00Z
2015-06-17T12:14:33Z
The designed construct was ordered from as a GeneArt?? String (Invitrogen??? Life Technologies).
This CBD is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus (UniProt ID Q06851 link http://www.uniprot.org/uniprot/Q06851). It is in RFC25 format to allow for easy use in protein fusions.
The CBD is part of the CBM3 family (link to cazy http://www.cazy.org/CBM3.html). This CBD has been used in many application: in fusions with cell adhesion peptides to enhance the properties of cellulose as a cell-growth matrix (Andrade <i>et al </i> 2010a, Andrade <i>et al </i> 2010b), fused to enzymes to remove contaminants from water (Kauffmann <i> et al </i> 2000) and fused to an antimicrobial peptide (Ramos, Domingues & Gama 2010).
At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.
false
false
_1696_
4206
20780
9
Not in stock
true
The DNA sequence from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 was codon optimised for ''E.coli''. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
false
Xenia Spencer-Milnes
annotation2420186
1
CBDcipA
range2420186
1
127
603
annotation2420187
1
Endogenous Linker (C-term)
range2420187
1
604
711
annotation2420141
1
Endogenous Linker (N-term)
range2420141
1
1
126
BBa_K1321096
1
BBa_K1321096
Linker-CBDcipA-Linker + Phytochelatin (PC) EC20
2014-10-16T11:00:00Z
2015-06-17T12:35:09Z
Please see part pages for the composite parts
Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
false
false
_1696_
4206
20780
9
Not in stock
false
The linker sequences mean fusions should be fine either N or N-terminally to the CBDcipA
false
Xenia Spencer-Milnes
component2423603
1
BBa_K1321014
component2423607
1
BBa_K1321005
component2423605
1
BBa_B0105
annotation2423605
1
BBa_B0105
range2423605
1
712
717
annotation2423603
1
BBa_K1321014
range2423603
1
1
711
annotation2423607
1
BBa_K1321005
range2423607
1
718
840
BBa_B0105
1
Scar 25
RFC 25 Scar Sequence
2009-10-14T11:00:00Z
2015-08-31T04:07:21Z
BBF RFC 25
This is the scar produced by assembly using RFC 25.
If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
false
false
_1_
0
25
397
Not in stock
false
Simple DNA sequence
false
Randy Rettberg
annotation2041621
1
Scar 25
range2041621
1
1
6
BBa_K1321096_sequence
1
aatgctacgccaactaagggtgcaaccccgaccaacacagcaacgcctacaaaaagcgctacagcaacacctacaagaccgtcagttcctacaaacacaccgactaacacaccggcaaatacacctgtttcaggcaacttgaaggtcgaattctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttccgtcaacacagccggtaacaaccccaccggcaacaaccaagccgccagcaacaaccaaaccaccggctaccacgattcctccatcagacgatccgaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
BBa_B0105_sequence
1
accggc
BBa_K1321005_sequence
1
gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
BBa_K1321014_sequence
1
aatgctacgccaactaagggtgcaaccccgaccaacacagcaacgcctacaaaaagcgctacagcaacacctacaagaccgtcagttcctacaaacacaccgactaacacaccggcaaatacacctgtttcaggcaacttgaaggtcgaattctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttccgtcaacacagccggtaacaaccccaccggcaacaaccaagccgccagcaacaaccaaaccaccggctaccacgattcctccatcagacgatccg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z