LOCUS       BBa_K1321100             553 bp    DNA     linear       17-May-2024
DEFINITION  Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter
ACCESSION   BBa_K1321100
VERSION     BBa_K1321100.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             1..43
                     /label=BBa_K1321338
     misc_feature    50..52
                     /label=start
     misc_feature    44..58
                     /label=BBa_K1319106
     CDS             59..181
                     /label=Synthetic Phytochelatin EC(20)
     CDS             59..181
                     /label=BBa_K1321005
     misc_feature    182..187
                     /label=Scar 25
     misc_feature    182..187
                     /label=BBa_B0105
     CDS             188..553
                     /label=dCBD
     CDS             188..259
                     /label=Linker
     misc_feature    260..373
                     /label=cbh2 CBD
     CDS             374..445
                     /label=Linker
     misc_feature    446..553
                     /label=cbh1 CBD
     CDS             188..553
                     /label=BBa_K1321340
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggccggcga
       61 atgtgaatgt gagtgcgaat gcgagtgtga atgtgagtgt gagtgtgaat gtgaatgcga
      121 gtgtgagtgc gagtgcgaat gtgagtgtga atgcgagtgc gaatgcgaat gcgagtgcgg
      181 caccggcccc ggcgccaatc cgcccggtac tacgaccact tcgcgtccag caaccacgac
      241 cgggtcgagc cccggcccac aggcctgttc ttccgtctgg ggtcagtgcg gcggacagaa
      301 ctggtctggc cctacctgct gtgcttctgg cagcacatgc gtctactcta acgactacta
      361 tagccagtgc ctgccgggtg ctaacccgcc aggcactacc actacctctc gccccgctac
      421 cactacaggc tctagcccgg gacctaccca aagccactac ggccagtgcg gaggcattgg
      481 ctacagcggc cctaccgtct gcgcctccgg aaccacctgc caagtcctga acccgtacta
      541 ctcccagtgt ctt
//