LOCUS BBa_K1321106 898 bp DNA linear 26-Dec-2024 DEFINITION Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter ACCESSION BBa_K1321106 VERSION BBa_K1321106.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 CDS 1..43 /label=BBa_K1321338 misc_feature 50..52 /label=start misc_feature 44..58 /label=BBa_K1319106 CDS 59..184 /label=Endogenous Linker (N-term) CDS 185..661 /label=CBDcipA CDS 662..769 /label=Endogenous Linker (C-term) CDS 59..769 /label=BBa_K1321014 misc_feature 770..775 /label=Scar 25 misc_feature 770..775 /label=BBa_B0105 CDS 776..898 /label=Synthetic Phytochelatin EC(20) CDS 776..898 /label=BBa_K1321005 CDS 59..898 /label=BBa_K1321096 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggccggcaa 61 tgctacgcca actaagggtg caaccccgac caacacagca acgcctacaa aaagcgctac 121 agcaacacct acaagaccgt cagttcctac aaacacaccg actaacacac cggcaaatac 181 acctgtttca ggcaacttga aggtcgaatt ctataactca aatccgagtg atacaactaa 241 cagtattaat ccgcagttta aagtaacaaa tacaggatca agtgcaattg atctttcaaa 301 gcttacattg agatactatt acaccgttga tggccagaag gaccagactt tctggtgtga 361 ccatgcagct atcataggta gcaacggctc atacaacggc atcacatcaa atgtaaaagg 421 aacattcgta aagatgagct caagcacaaa taacgcagac acatacctcg aaataagttt 481 cacaggtggc actttggaac ctggtgctca tgtacagata cagggtaggt ttgcgaaaaa 541 tgactggagt aattatacac agtcaaatga ttactcattt aagtcagcat cacagttcgt 601 agaatgggat caggttacag catatttgaa tggagtactt gtatggggta aagaaccagg 661 aggatcagta gttccgtcaa cacagccggt aacaacccca ccggcaacaa ccaagccgcc 721 agcaacaacc aaaccaccgg ctaccacgat tcctccatca gacgatccga ccggcgaatg 781 tgaatgtgag tgcgaatgcg agtgtgaatg tgagtgtgag tgtgaatgtg aatgcgagtg 841 tgagtgcgag tgcgaatgtg agtgtgaatg cgagtgcgaa tgcgaatgcg agtgcggc //