LOCUS       BBa_K1321110             730 bp    DNA     linear       22-Dec-2024
DEFINITION  Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
ACCESSION   BBa_K1321110
VERSION     BBa_K1321110.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..358
                     /label=Synthetic Phytochelatin EC(20)
     CDS             236..358
                     /label=BBa_K1321005
     misc_feature    359..364
                     /label=Scar 25
     misc_feature    359..364
                     /label=BBa_B0105
     CDS             365..730
                     /label=dCBD
     CDS             365..436
                     /label=Linker
     misc_feature    437..550
                     /label=cbh2 CBD
     CDS             551..622
                     /label=Linker
     misc_feature    623..730
                     /label=cbh1 CBD
     CDS             365..730
                     /label=BBa_K1321340
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcgaatg
      241 tgaatgtgag tgcgaatgcg agtgtgaatg tgagtgtgag tgtgaatgtg aatgcgagtg
      301 tgagtgcgag tgcgaatgtg agtgtgaatg cgagtgcgaa tgcgaatgcg agtgcggcac
      361 cggccccggc gccaatccgc ccggtactac gaccacttcg cgtccagcaa ccacgaccgg
      421 gtcgagcccc ggcccacagg cctgttcttc cgtctggggt cagtgcggcg gacagaactg
      481 gtctggccct acctgctgtg cttctggcag cacatgcgtc tactctaacg actactatag
      541 ccagtgcctg ccgggtgcta acccgccagg cactaccact acctctcgcc ccgctaccac
      601 tacaggctct agcccgggac ctacccaaag ccactacggc cagtgcggag gcattggcta
      661 cagcggccct accgtctgcg cctccggaac cacctgccaa gtcctgaacc cgtactactc
      721 ccagtgtctt
//