BBa_K1321110
type: ComponentDefinition
persistent identity: BBa K1321110
display id: BBa_K1321110
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:00Z
mutable provenance: Phytochelatin EC(20) +CBD2 with LacI
owned by: james
owned by: myers
mutable description: Phytochelatin EC(20) +CBD2 with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Phytochelatin EC(20) +CBD2 with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1321005
component: BBa_K1319106
component: BBa_K1321340
component: BBa_B0105
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321110_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414866
display id: component2414866
version: 1
top level: BBa_K1321110
definition: BBa_K1321005
access: public

BBa_K1319106
type: Component
persistent identity: component2414864
display id: component2414864
version: 1
top level: BBa_K1321110
definition: Scar25 ATG
access: public

BBa_K1321340
type: Component
persistent identity: component2414870
display id: component2414870
version: 1
top level: BBa_K1321110
definition: BBa_K1321340
access: public

BBa_B0105
type: Component
persistent identity: component2414868
display id: component2414868
version: 1
top level: BBa_K1321110
definition: Scar 25
access: public

BBa_J04500
type: Component
persistent identity: component2414862
display id: component2414862
version: 1
top level: BBa_K1321110
definition: BBa_J04500
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414862
display id: annotation2414862
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
type: Range
persistent identity: range2414862
display id: range2414862
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414864
display id: annotation2414864
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414864/range2414864/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414864/range2414864/1
type: Range
persistent identity: range2414864
display id: range2414864
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414866
display id: annotation2414866
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
type: Range
persistent identity: range2414866
display id: range2414866
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 236
end: 358
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414868
display id: annotation2414868
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
type: Range
persistent identity: range2414868
display id: range2414868
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 359
end: 364
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414870
display id: annotation2414870
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414870/range2414870/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414870/range2414870/1
type: Range
persistent identity: range2414870
display id: range2414870
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 365
end: 730
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

Scar25 ATG
type: ComponentDefinition
persistent identity: BBa K1319106
display id: BBa_K1319106
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Start Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1319106_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2393317
display id: annotation2393317
version: 1
top level: Scar25 ATG
location: https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
type: Range
persistent identity: range2393317
display id: range2393317
version: 1
top level: Scar25 ATG
direction: direction/forward
start: 7
end: 9
orientation: inline

BBa_K1321005
type: ComponentDefinition
persistent identity: BBa K1321005
display id: BBa_K1321005
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Phytochelatin EC(20) RFC[25]
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: Sequence is not natural. Basis for the sequence was from the following reference: Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.
owned by: james
owned by: myers
mutable description: Synthetic Phytochelatin EC(20) rfc25 fusion format
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Codon optimised for E.coli.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Synthetic Phytochelatin EC(20)
sequence: https://synbiohub.org/public/igem/BBa_K1321005_sequence/1

Synthetic Phytochelatin EC(20)
type: SequenceAnnotation
persistent identity: annotation2414598
display id: annotation2414598
version: 1
top level: BBa_K1321005
location: https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1
type: Range
persistent identity: range2414598
display id: range2414598
version: 1
top level: BBa_K1321005
direction: direction/forward
start: 1
end: 123
orientation: inline

BBa_K1321340
type: ComponentDefinition
persistent identity: BBa K1321340
display id: BBa_K1321340
version: 1
was generated by: iGEM to SBOL conversion
description: Double CBD (dCBD) with N-terminal linker
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:46Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: dCBD with N-terminal linker.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCBD
sequence annotation: Linker
sequence annotation: cbh2 CBD
sequence annotation: cbh1 CBD
sequence: https://synbiohub.org/public/igem/BBa_K1321340_sequence/1

dCBD
type: SequenceAnnotation
persistent identity: annotation2417847
display id: annotation2417847
version: 1
top level: BBa_K1321340
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
type: Range
persistent identity: range2417847
display id: range2417847
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 1
end: 366
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417848
display id: annotation2417848
version: 1
top level: BBa_K1321340
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
type: Range
persistent identity: range2417848
display id: range2417848
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 1
end: 72
orientation: inline

cbh2 CBD
type: SequenceAnnotation
persistent identity: annotation2417849
display id: annotation2417849
version: 1
top level: BBa_K1321340
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
type: Range
persistent identity: range2417849
display id: range2417849
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 73
end: 186
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417850
display id: annotation2417850
version: 1
top level: BBa_K1321340
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
type: Range
persistent identity: range2417850
display id: range2417850
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 187
end: 258
orientation: inline

cbh1 CBD
type: SequenceAnnotation
persistent identity: annotation2417851
display id: annotation2417851
version: 1
top level: BBa_K1321340
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
type: Range
persistent identity: range2417851
display id: range2417851
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 259
end: 366
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

https://synbiohub.org/public/igem/BBa_K1321110_sequence/1
type: Sequence
persistent identity: BBa K1321110 sequence
display id: BBa_K1321110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319106_sequence/1
type: Sequence
persistent identity: BBa K1319106 sequence
display id: BBa_K1319106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321005_sequence/1
type: Sequence
persistent identity: BBa K1321005 sequence
display id: BBa_K1321005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321340_sequence/1
type: Sequence
persistent identity: BBa K1321340 sequence
display id: BBa_K1321340_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z