LOCUS       BBa_K1321113            1075 bp    DNA     linear       27-Dec-2024
DEFINITION  Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter
ACCESSION   BBa_K1321113
VERSION     BBa_K1321113.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..358
                     /label=Synthetic Phytochelatin EC(20)
     CDS             236..358
                     /label=BBa_K1321005
     misc_feature    359..364
                     /label=Scar 25
     misc_feature    359..364
                     /label=BBa_B0105
     CDS             365..490
                     /label=Endogenous Linker (N-term)
     CDS             491..967
                     /label=CBDcipA
     CDS             968..1075
                     /label=Endogenous Linker (C-term)
     CDS             365..1075
                     /label=BBa_K1321014
     CDS             236..1075
                     /label=BBa_K1321093
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcgaatg
      241 tgaatgtgag tgcgaatgcg agtgtgaatg tgagtgtgag tgtgaatgtg aatgcgagtg
      301 tgagtgcgag tgcgaatgtg agtgtgaatg cgagtgcgaa tgcgaatgcg agtgcggcac
      361 cggcaatgct acgccaacta agggtgcaac cccgaccaac acagcaacgc ctacaaaaag
      421 cgctacagca acacctacaa gaccgtcagt tcctacaaac acaccgacta acacaccggc
      481 aaatacacct gtttcaggca acttgaaggt cgaattctat aactcaaatc cgagtgatac
      541 aactaacagt attaatccgc agtttaaagt aacaaataca ggatcaagtg caattgatct
      601 ttcaaagctt acattgagat actattacac cgttgatggc cagaaggacc agactttctg
      661 gtgtgaccat gcagctatca taggtagcaa cggctcatac aacggcatca catcaaatgt
      721 aaaaggaaca ttcgtaaaga tgagctcaag cacaaataac gcagacacat acctcgaaat
      781 aagtttcaca ggtggcactt tggaacctgg tgctcatgta cagatacagg gtaggtttgc
      841 gaaaaatgac tggagtaatt atacacagtc aaatgattac tcatttaagt cagcatcaca
      901 gttcgtagaa tgggatcagg ttacagcata tttgaatgga gtacttgtat ggggtaaaga
      961 accaggagga tcagtagttc cgtcaacaca gccggtaaca accccaccgg caacaaccaa
     1021 gccgccagca acaaccaaac caccggctac cacgattcct ccatcagacg atccg
//