LOCUS       BBa_K1321114             664 bp    DNA     linear       22-Dec-2024
DEFINITION  CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
ACCESSION   BBa_K1321114
VERSION     BBa_K1321114.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..535
                     /label=BBa_K1321002
     misc_feature    536..541
                     /label=Scar 25
     misc_feature    536..541
                     /label=BBa_B0105
     CDS             542..664
                     /label=Synthetic Phytochelatin EC(20)
     CDS             542..664
                     /label=BBa_K1321005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggctcatc
      241 aatgtcagtt gaattttaca actctaacaa atcagcacaa acaaactcaa ttacaccaat
      301 aatcaaaatt actaacacgt ctgacagtga tttaaattta aatgacgtaa aagttagata
      361 ttattacaca agtgatggta cacaaggaca aactttctgg tgtgaccatg ctggtgcatt
      421 attaggaaat agctatgttg ataacactag caaagtgaca gcaaacttcg ttaaagaaac
      481 agcaagccca acatcaacct atgatacata tgttgaattt ggatttgcag gcagcaccgg
      541 cgaatgtgaa tgtgagtgcg aatgcgagtg tgaatgtgag tgtgagtgtg aatgtgaatg
      601 cgagtgtgag tgcgagtgcg aatgtgagtg tgaatgcgag tgcgaatgcg aatgcgagtg
      661 cggc
//