LOCUS       BBa_K1321116            1075 bp    DNA     linear       19-Apr-2024
DEFINITION  Linker-cipA-linker + phytochelatin (PC) EC20 with LacI promoter
ACCESSION   BBa_K1321116
VERSION     BBa_K1321116.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..361
                     /label=Endogenous Linker (N-term)
     CDS             362..838
                     /label=CBDcipA
     CDS             839..946
                     /label=Endogenous Linker (C-term)
     CDS             236..946
                     /label=BBa_K1321014
     misc_feature    947..952
                     /label=Scar 25
     misc_feature    947..952
                     /label=BBa_B0105
     CDS             953..1075
                     /label=Synthetic Phytochelatin EC(20)
     CDS             953..1075
                     /label=BBa_K1321005
     CDS             236..1075
                     /label=BBa_K1321096
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcaatgc
      241 tacgccaact aagggtgcaa ccccgaccaa cacagcaacg cctacaaaaa gcgctacagc
      301 aacacctaca agaccgtcag ttcctacaaa cacaccgact aacacaccgg caaatacacc
      361 tgtttcaggc aacttgaagg tcgaattcta taactcaaat ccgagtgata caactaacag
      421 tattaatccg cagtttaaag taacaaatac aggatcaagt gcaattgatc tttcaaagct
      481 tacattgaga tactattaca ccgttgatgg ccagaaggac cagactttct ggtgtgacca
      541 tgcagctatc ataggtagca acggctcata caacggcatc acatcaaatg taaaaggaac
      601 attcgtaaag atgagctcaa gcacaaataa cgcagacaca tacctcgaaa taagtttcac
      661 aggtggcact ttggaacctg gtgctcatgt acagatacag ggtaggtttg cgaaaaatga
      721 ctggagtaat tatacacagt caaatgatta ctcatttaag tcagcatcac agttcgtaga
      781 atgggatcag gttacagcat atttgaatgg agtacttgta tggggtaaag aaccaggagg
      841 atcagtagtt ccgtcaacac agccggtaac aaccccaccg gcaacaacca agccgccagc
      901 aacaaccaaa ccaccggcta ccacgattcc tccatcagac gatccgaccg gcgaatgtga
      961 atgtgagtgc gaatgcgagt gtgaatgtga gtgtgagtgt gaatgtgaat gcgagtgtga
     1021 gtgcgagtgc gaatgtgagt gtgaatgcga gtgcgaatgc gaatgcgagt gcggc
//