LOCUS       BBa_K1321201            3405 bp    DNA     linear       29-Apr-2024
DEFINITION  VHb in pSEVA331-Bb
ACCESSION   BBa_K1321201
VERSION     BBa_K1321201.1  
FEATURES             Location/Qualifiers
     RBS             44..55
                     /label=B0034
     promoter        1..35
                     /label=J23101 constitutive
     misc_feature    64..66
                     /label=Start
     misc_feature    502..504
                     /label=Stop
     CDS             64..504
                     /label=VHb
     misc_feature    1..504
                     /label=BBa_K1321200
     misc_feature    513..534
                     /label=Biobrick suffix
     misc_feature    3384..3405
                     /label=Biobrick prefix
     CDS             783..1442
                     /label=Chloramphenicol acetyltransferase
     misc_feature    1466..1701
                     /label=OriT
     misc_feature    1710..2372
                     /label=Rep
     misc_feature    513..3405
                     /label=BBa_K1321300
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagatgctgg atcagcagac cgttgatacc agcaaagcaa ccgttccggt tctgaaagaa
      121 catggtgtta ccattaccac caccttttat cagaacctgt ttgcaaaaca tccggaagtt
      181 cgtccgctgt ttgatatggg tcgtcaggca agcctggaac agccgaaagc actggcaatg
      241 accgttggtg cagcagcaca gaatattgaa aatctgcctg caattctgcc tgccgttcag
      301 aaaattgcag ttaaacattg tcaggcaggc gttgcagcac gtcattatcc gattgttggt
      361 caagaactgc tgggtgcaat taaagagctg ctgggcgacg cagcaaccga tgatattctg
      421 gatgcatggg gtaaagcata tggtgttatt gccgatgttt ttattcaggt tgaagcagat
      481 ctgtatgcac aggatgcaga ataatactag agtactagta gcggccgctg cagcttggac
      541 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
      601 gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa
      661 attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
      721 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
      781 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
      841 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
      901 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
      961 ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg
     1021 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     1081 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     1141 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     1201 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     1261 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     1321 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     1381 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     1441 aatttgactt ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt
     1501 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
     1561 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
     1621 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
     1681 tgctctgcga ggctggccgt aggccggccc taccggcgcg gcagcgttac ccgtgtcggc
     1741 ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc
     1801 tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc
     1861 ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc
     1921 ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg
     1981 tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg
     2041 ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc
     2101 acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa
     2161 gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc
     2221 atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg
     2281 gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct
     2341 tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc
     2401 acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc
     2461 gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg
     2521 gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct
     2581 ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt
     2641 cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg
     2701 atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac
     2761 tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc
     2821 gcataggctt gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga
     2881 gccttgaccc gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc
     2941 ggaggctttt ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag
     3001 ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa
     3061 gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag
     3121 cgaagcgagc cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag
     3181 ggagcgcagc gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg gggcgcgccc
     3241 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3301 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tttaattaaa
     3361 gcggataaca atttcacaca ggagaattcg cggccgcttc tagag
//