##gff-version 3 ##sequence-region BBa_K1321201 1 3405 BBa_K1321201 . plasmid_vector 513 3405 . + 0 ID=BBa_K1321300;Name=BBa_K1321300 BBa_K1321201 . sequence_feature 1710 2372 . + 0 ID=annotation2412990;Name=Rep;Parent=BBa_K1321300 BBa_K1321201 . sequence_feature 1466 1701 . + 0 ID=annotation2412975;Name=OriT;Parent=BBa_K1321300 BBa_K1321201 . sequence_feature 513 534 . + 0 ID=annotation2408497;Name=Biobrick suffix;Parent=BBa_K1321300 BBa_K1321201 . sequence_feature 3384 3405 . + 0 ID=annotation2408614;Name=Biobrick prefix;Parent=BBa_K1321300 BBa_K1321201 . CDS 783 1442 . + 0 ID=annotation2408619;Name=Chloramphenicol acetyltransferase;Parent=BBa_K1321300 BBa_K1321201 . engineered_region 1 504 . + 0 ID=BBa_K1321200;Name=BBa_K1321200 BBa_K1321201 . stop_codon 502 504 . + 0 ID=annotation2422497;Name=Stop;Parent=BBa_K1321200 BBa_K1321201 . ribosome_entry_site 44 55 . + 0 ID=annotation2422493;Name=B0034;Parent=BBa_K1321200 BBa_K1321201 . start_codon 64 66 . + 0 ID=annotation2422496;Name=Start;Parent=BBa_K1321200 BBa_K1321201 . CDS 64 504 . + 0 ID=annotation2422498;Name=VHb;Parent=BBa_K1321200 BBa_K1321201 . promoter 1 35 . + 0 ID=annotation2422494;Name=J23101 constitutive;Parent=BBa_K1321200 >BBa_K1321201 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagatgctggatcagcaga ccgttgataccagcaaagcaaccgttccggttctgaaagaacatggtgttaccattaccaccaccttttatcagaacct gtttgcaaaacatccggaagttcgtccgctgtttgatatgggtcgtcaggcaagcctggaacagccgaaagcactggca atgaccgttggtgcagcagcacagaatattgaaaatctgcctgcaattctgcctgccgttcagaaaattgcagttaaac attgtcaggcaggcgttgcagcacgtcattatccgattgttggtcaagaactgctgggtgcaattaaagagctgctggg cgacgcagcaaccgatgatattctggatgcatggggtaaagcatatggtgttattgccgatgtttttattcaggttgaa gcagatctgtatgcacaggatgcagaataatactagagtactagtagcggccgctgcagcttggactcctgttgataga tccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat ccaggggtccccaataattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcacc ataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaa aaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgct caatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagtttt atccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagct ggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaa taccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttcc ctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggc caatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctg gcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatg agtggcagggcggggcgtaatttgacttttgtccttttccgctgcataaccctgcttcggggtcattatagcgattttt tcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaac ggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacct ggcggtgctcaacgggaatcctgctctgcgaggctggccgtaggccggccctaccggcgcggcagcgttacccgtgtcg gcggctccaacggctcgccatcgtccagaaaacacggctcatcgggcatcggcaggcgctgctgcccgcgccgttccca ttcctccgtttcggtcaaggctggcaggtctggttccatgcccggaatgccgggctggctgggcggctcctcgccgggg ccggtcggtagttgctgctcgcccggatacagggtcgggatgcggcgcaggtcgccatgccccaacagcgattcgtcct ggtcgtcgtgatcaaccaccacggcggcactgaacaccgacaggcgcaactggtcgcggggctggccccacgccacgcg gtcattgaccacgtaggccgacacggtgccggggccgttgagcttcacgacggagatccagcgctcggccaccaagtcc ttgactgcgtattggaccgtccgcaaagaacgtccgatgagcttggaaagtgtcttctggctgaccaccacggcgttct ggtggcccatctgcgccacgaggtgatgcagcagcattgccgccgtgggtttcctcgcaataagcccggcccacgcctc atgcgctttgcgttccgtttgcacccagtgaccgggcttgttcttggcttgaatgccgatttctctggactgcgtggcc atgcttatctccatgcggtaggggtgccgcacggttgcggcaccatgcgcaatcagctgcaacttttcggcagcgcgac aacaattatgcgttgcgtaaaagtggcagtcaattacagattttctttaacctacgcaatgagctattgcggggggtgc cgcaatgagctgttgcgtaccccccttttttaagttgttgatttttaagtctttcgcatttcgccctatatctagttct ttggtgcccaaagaagggcacccctgcggggttcccccacgccttcggcgcggctccccctccggcaaaaagtggcccc tccggggcttgttgatcgactgcgcggccttcggccttgcccaaggtggcgctgcccccttggaacccccgcactcgcc gccgtgaggctcggggggcaggcgggcgggcttcgcccttcgactgcccccactcgcataggcttgggtcgttccaggc gcgtcaaggccaagccgctgcgcggtcgctgcgcgagccttgacccgccttccacttggtgtccaaccggcaagcgaag cgcgcaggccgcaggccggaggcttttccccagagaaaattaaaaaaattgatggggcaaggccgcaggccgcgcagtt ggagccggtgggtatgtggtcgaaggctgggtagccggtgggcaatccctgtggtcaagctcgtgggcaggcgcagcct gtccatcagcttgtccagcagggttgtccacgggccgagcgaagcgagccagccggtggccgctcgcggccatcgtcca catatccacgggctggcaagggagcgcagcgaccgcgcagggcgaagcccggagagcaagcccgtagggggggcgcgcc cagctgtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtc tttcgactgagcctttcgttttatttgatgcctttaattaaagcggataacaatttcacacaggagaattcgcggccgc ttctagag