LOCUS BBa_K1321202 4105 bp DNA linear 22-Nov-2024 DEFINITION VHb in pSEVA321-Bb ACCESSION BBa_K1321202 VERSION BBa_K1321202.1 FEATURES Location/Qualifiers RBS 44..55 /label=B0034 promoter 1..35 /label=J23101 constitutive misc_feature 64..66 /label=Start misc_feature 502..504 /label=Stop CDS 64..504 /label=VHb misc_feature 1..504 /label=BBa_K1321200 misc_feature 513..533 /label=Biobrick suffix misc_feature 4084..4105 /label=Biobrick prefix CDS 783..1442 /label=Chloramphenicol acetyltransferase misc_feature 1466..1701 /label=OriT misc_feature 1760..2908 /label=Replication protein TrfA misc_feature 513..4105 /label=BBa_K1321301 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gagatgctgg atcagcagac cgttgatacc agcaaagcaa ccgttccggt tctgaaagaa 121 catggtgtta ccattaccac caccttttat cagaacctgt ttgcaaaaca tccggaagtt 181 cgtccgctgt ttgatatggg tcgtcaggca agcctggaac agccgaaagc actggcaatg 241 accgttggtg cagcagcaca gaatattgaa aatctgcctg caattctgcc tgccgttcag 301 aaaattgcag ttaaacattg tcaggcaggc gttgcagcac gtcattatcc gattgttggt 361 caagaactgc tgggtgcaat taaagagctg ctgggcgacg cagcaaccga tgatattctg 421 gatgcatggg gtaaagcata tggtgttatt gccgatgttt ttattcaggt tgaagcagat 481 ctgtatgcac aggatgcaga ataatactag agtactagta gcggccgctg cagcttggac 541 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg 601 gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa 661 attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa 721 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta 781 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag 841 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg 901 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta 961 ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg 1021 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg 1081 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat 1141 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg 1201 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg 1261 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag 1321 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc 1381 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt 1441 aatttgactt ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt 1501 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga 1561 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc 1621 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc 1681 tgctctgcga ggctggccgt aggccggccg cgatgcaggt ggctgctgaa cccccagccg 1741 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt 1801 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact 1861 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt 1921 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc 1981 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct 2041 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt 2101 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg 2161 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc 2221 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct 2281 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc 2341 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct 2401 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca 2461 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga 2521 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ttcgctgacc tgttttgcca 2581 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca 2641 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg 2701 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag 2761 cttgctggac tatcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc 2821 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt 2881 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccggaattaa 2941 ttccccggat cgatccgtcg atcttgatcc cctgcgccat cagatccttg gcggcaagaa 3001 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa 3061 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact 3121 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct 3181 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgg ctgccatttt 3241 tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt 3301 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc 3361 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa 3421 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc 3481 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct 3541 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc 3601 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg 3661 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc 3721 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc 3781 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccctacatgg 3841 ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcccccgc agaaaaaaag 3901 gatctcaaga agatcctttg atcttttcta cggcgcgccc agctgtctag ggcggcggat 3961 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 4021 tcgactgagc ctttcgtttt atttgatgcc tttaattaaa gcggataaca atttcacaca 4081 ggagaattcg cggccgcttc tagag //