##gff-version 3
##sequence-region BBa_K1321295 1 4153
BBa_K1321295	.	CDS	1	1252	.	+	0	ID=BBa_K1321360;Name=BBa_K1321360
BBa_K1321295	.	CDS	236	1252	.	+	0	ID=BBa_K1321346;Name=BBa_K1321346;Parent=BBa_K1321360
BBa_K1321295	.	CDS	542	1252	.	+	0	ID=BBa_K1321337;Name=BBa_K1321337;Parent=BBa_K1321346
BBa_K1321295	.	CDS	542	1252	.	+	0	ID=annotation2417826;Name=sfGFP;Parent=BBa_K1321337
BBa_K1321295	.	sequence_feature	536	541	.	+	0	ID=BBa_B0105;Name=BBa_B0105;Parent=BBa_K1321346
BBa_K1321295	.	sequence_feature	536	541	.	+	0	ID=annotation2041621;Name=Scar 25;Parent=BBa_B0105
BBa_K1321295	.	CDS	236	535	.	+	0	ID=BBa_K1321002;Name=BBa_K1321002;Parent=BBa_K1321346
BBa_K1321295	.	sequence_feature	221	235	.	+	0	ID=BBa_K1319106;Name=BBa_K1319106;Parent=BBa_K1321360
BBa_K1321295	.	start_codon	227	229	.	+	0	ID=annotation2393317;Name=start;Parent=BBa_K1319106
BBa_K1321295	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1321360
BBa_K1321295	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1321295	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1321295	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1321295	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1321295	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1321295	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1321295	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1321295	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1321295	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1321295	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1321295	.	plasmid_vector	1261	4153	.	+	0	ID=BBa_K1321300;Name=BBa_K1321300
BBa_K1321295	.	sequence_feature	4132	4153	.	+	0	ID=annotation2408614;Name=Biobrick prefix;Parent=BBa_K1321300
BBa_K1321295	.	sequence_feature	1261	1282	.	+	0	ID=annotation2408497;Name=Biobrick suffix;Parent=BBa_K1321300
BBa_K1321295	.	sequence_feature	2214	2449	.	+	0	ID=annotation2412975;Name=OriT;Parent=BBa_K1321300
BBa_K1321295	.	sequence_feature	2458	3120	.	+	0	ID=annotation2412990;Name=Rep;Parent=BBa_K1321300
BBa_K1321295	.	CDS	1531	2190	.	+	0	ID=annotation2408619;Name=Chloramphenicol acetyltransferase;Parent=BBa_K1321300
>BBa_K1321295
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggctc
atcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaac
acgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactt
tctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaa
agaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggccgtaaaggcgaa
gagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcg
agggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggcc
gactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttc
aagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcgg
aagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcct
gggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcg
aattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtg
atggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcga
tcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatactagagtact
agtagcggccgctgcagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaac
gctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatga
gacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttat
cgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggca
tcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcc
tttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctc
atccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttcca
tgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaa
gatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatc
cctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaa
atattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtc
ggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgacttttgtccttttccgc
tgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgc
caaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcga
gcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgta
ggccggccctaccggcgcggcagcgttacccgtgtcggcggctccaacggctcgccatcgtccagaaaacacggctcat
cgggcatcggcaggcgctgctgcccgcgccgttcccattcctccgtttcggtcaaggctggcaggtctggttccatgcc
cggaatgccgggctggctgggcggctcctcgccggggccggtcggtagttgctgctcgcccggatacagggtcgggatg
cggcgcaggtcgccatgccccaacagcgattcgtcctggtcgtcgtgatcaaccaccacggcggcactgaacaccgaca
ggcgcaactggtcgcggggctggccccacgccacgcggtcattgaccacgtaggccgacacggtgccggggccgttgag
cttcacgacggagatccagcgctcggccaccaagtccttgactgcgtattggaccgtccgcaaagaacgtccgatgagc
ttggaaagtgtcttctggctgaccaccacggcgttctggtggcccatctgcgccacgaggtgatgcagcagcattgccg
ccgtgggtttcctcgcaataagcccggcccacgcctcatgcgctttgcgttccgtttgcacccagtgaccgggcttgtt
cttggcttgaatgccgatttctctggactgcgtggccatgcttatctccatgcggtaggggtgccgcacggttgcggca
ccatgcgcaatcagctgcaacttttcggcagcgcgacaacaattatgcgttgcgtaaaagtggcagtcaattacagatt
ttctttaacctacgcaatgagctattgcggggggtgccgcaatgagctgttgcgtaccccccttttttaagttgttgat
ttttaagtctttcgcatttcgccctatatctagttctttggtgcccaaagaagggcacccctgcggggttcccccacgc
cttcggcgcggctccccctccggcaaaaagtggcccctccggggcttgttgatcgactgcgcggccttcggccttgccc
aaggtggcgctgcccccttggaacccccgcactcgccgccgtgaggctcggggggcaggcgggcgggcttcgcccttcg
actgcccccactcgcataggcttgggtcgttccaggcgcgtcaaggccaagccgctgcgcggtcgctgcgcgagccttg
acccgccttccacttggtgtccaaccggcaagcgaagcgcgcaggccgcaggccggaggcttttccccagagaaaatta
aaaaaattgatggggcaaggccgcaggccgcgcagttggagccggtgggtatgtggtcgaaggctgggtagccggtggg
caatccctgtggtcaagctcgtgggcaggcgcagcctgtccatcagcttgtccagcagggttgtccacgggccgagcga
agcgagccagccggtggccgctcgcggccatcgtccacatatccacgggctggcaagggagcgcagcgaccgcgcaggg
cgaagcccggagagcaagcccgtagggggggcgcgcccagctgtctagggcggcggatttgtcctactcaggagagcgt
tcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctttaattaaa
gcggataacaatttcacacaggagaattcgcggccgcttctagag