LOCUS BBa_K1321296 4153 bp DNA linear 22-Nov-2024 DEFINITION sfGFP fused to CBDclos driven by LacI in pSEVA331-Bb ACCESSION BBa_K1321296 VERSION BBa_K1321296.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 misc_feature 227..229 /label=start misc_feature 221..235 /label=BBa_K1319106 CDS 236..946 /label=sfGFP CDS 236..946 /label=BBa_K1321337 misc_feature 947..952 /label=Scar 25 misc_feature 947..952 /label=BBa_B0105 CDS 953..1252 /label=BBa_K1321002 CDS 236..1252 /label=BBa_K1321341 misc_feature 1..1252 /label=BBa_K1321356 misc_feature 1261..1282 /label=Biobrick suffix misc_feature 4132..4153 /label=Biobrick prefix CDS 1531..2190 /label=Chloramphenicol acetyltransferase misc_feature 2214..2449 /label=OriT misc_feature 2458..3120 /label=Rep misc_feature 1261..4153 /label=BBa_K1321300 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggccgtaa 241 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa 301 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac 361 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac 421 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt 481 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga 541 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat 601 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata 661 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc 721 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca 781 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac 841 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt 901 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaaccg gctcatcaat 961 gtcagttgaa ttttacaact ctaacaaatc agcacaaaca aactcaatta caccaataat 1021 caaaattact aacacgtctg acagtgattt aaatttaaat gacgtaaaag ttagatatta 1081 ttacacaagt gatggtacac aaggacaaac tttctggtgt gaccatgctg gtgcattatt 1141 aggaaatagc tatgttgata acactagcaa agtgacagca aacttcgtta aagaaacagc 1201 aagcccaaca tcaacctatg atacatatgt tgaatttgga tttgcaggca gctactagag 1261 tactagtagc ggccgctgca gcttggactc ctgttgatag atccagtaat gacctcagaa 1321 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat 1381 ccaggggtcc ccaataatta cgatttaaat tggcgaaaat gagacgttga tcggcacgta 1441 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 1501 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 1561 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 1621 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 1681 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 1741 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 1801 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 1861 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 1921 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 1981 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 2041 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 2101 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 2161 tactgcgatg agtggcaggg cggggcgtaa tttgactttt gtccttttcc gctgcataac 2221 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 2281 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 2341 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 2401 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcccta 2461 ccggcgcggc agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca 2521 cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt 2581 caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc 2641 ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc 2701 atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac 2761 cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc 2821 cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc 2881 cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg 2941 gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc 3001 cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg 3061 cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat 3121 gcttatctcc atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa 3181 cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt 3241 tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc 3301 cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt 3361 gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg 3421 caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg 3481 tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc 3541 gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag 3601 gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc 3661 ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat 3721 tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg 3781 gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct 3841 tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc 3901 atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg 3961 agagcaagcc cgtagggggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca 4021 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct 4081 ttcgttttat ttgatgcctt taattaaagc ggataacaat ttcacacagg agaattcgcg 4141 gccgcttcta gag //