LOCUS BBa_K1321297 4180 bp DNA linear 22-Nov-2024 DEFINITION sfGFP fused to CBDcex driven by LacI in pSEVA331-Bb ACCESSION BBa_K1321297 VERSION BBa_K1321297.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 misc_feature 227..229 /label=start misc_feature 221..235 /label=BBa_K1319106 CDS 236..946 /label=sfGFP CDS 236..946 /label=BBa_K1321337 misc_feature 947..952 /label=Scar 25 misc_feature 947..952 /label=BBa_B0105 CDS 953..1279 /label=BBa_K1321003 CDS 236..1279 /label=BBa_K1321342 misc_feature 1..1279 /label=BBa_K1321357 misc_feature 1288..1309 /label=Biobrick suffix misc_feature 4159..4180 /label=Biobrick prefix CDS 1558..2217 /label=Chloramphenicol acetyltransferase misc_feature 2241..2476 /label=OriT misc_feature 2485..3147 /label=Rep misc_feature 1288..4180 /label=BBa_K1321300 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggccgtaa 241 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa 301 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac 361 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac 421 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt 481 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga 541 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat 601 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata 661 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc 721 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca 781 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac 841 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt 901 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaaccg gcggtccggc 961 cgggtgccag gtgctgtggg gcgtcaacca gtggaacacc ggcttcaccg cgaacgtcac 1021 cgtgaagaac acgtcctccg ctccggtaga cggctggacg ctcacgttca gcttcccgtc 1081 cggccagcag gtcacccagg cgtggagctc gacggtcacg cagtccggct cggccgtgac 1141 ggtccgcaac gccccgtgga acggctcgat cccggcgggc ggcaccgcgc agttcggctt 1201 caacggctcg cacacgggca ccaacgccgc gccgacggcg ttctcgctca acggcacgcc 1261 ctgcacggtc ggcggcagct actagagtac tagtagcggc cgctgcagct tggactcctg 1321 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc 1381 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttaaattgg 1441 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga 1501 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg 1561 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat 1621 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt 1681 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac 1741 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag 1801 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg 1861 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg 1921 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat 1981 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc 2041 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac 2101 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc 2161 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt 2221 gacttttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg 2281 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc 2341 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg 2401 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc 2461 tgcgaggctg gccgtaggcc ggccctaccg gcgcggcagc gttacccgtg tcggcggctc 2521 caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc 2581 gcgccgttcc cattcctccg tttcggtcaa ggctggcagg tctggttcca tgcccggaat 2641 gccgggctgg ctgggcggct cctcgccggg gccggtcggt agttgctgct cgcccggata 2701 cagggtcggg atgcggcgca ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg 2761 atcaaccacc acggcggcac tgaacaccga caggcgcaac tggtcgcggg gctggcccca 2821 cgccacgcgg tcattgacca cgtaggccga cacggtgccg gggccgttga gcttcacgac 2881 ggagatccag cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg 2941 tccgatgagc ttggaaagtg tcttctggct gaccaccacg gcgttctggt ggcccatctg 3001 cgccacgagg tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa gcccggccca 3061 cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat 3121 gccgatttct ctggactgcg tggccatgct tatctccatg cggtaggggt gccgcacggt 3181 tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc 3241 gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg 3301 tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc 3361 atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc 3421 acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga 3481 ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc 3541 gccgtgaggc tcggggggca ggcgggcggg cttcgccctt cgactgcccc cactcgcata 3601 ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt 3661 gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc aggccggagg 3721 cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg cgcagttgga 3781 gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg gtcaagctcg 3841 tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg ccgagcgaag 3901 cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg gcaagggagc 3961 gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggggggcg cgcccagctg 4021 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac 4081 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaaagcgga 4141 taacaatttc acacaggaga attcgcggcc gcttctagag //