##gff-version 3
##sequence-region BBa_K1321297 1 4180
BBa_K1321297	.	engineered_region	1	1279	.	+	0	ID=BBa_K1321357;Name=BBa_K1321357
BBa_K1321297	.	CDS	236	1279	.	+	0	ID=BBa_K1321342;Name=BBa_K1321342;Parent=BBa_K1321357
BBa_K1321297	.	sequence_feature	947	952	.	+	0	ID=BBa_B0105;Name=BBa_B0105;Parent=BBa_K1321342
BBa_K1321297	.	sequence_feature	947	952	.	+	0	ID=annotation2041621;Name=Scar 25;Parent=BBa_B0105
BBa_K1321297	.	CDS	953	1279	.	+	0	ID=BBa_K1321003;Name=BBa_K1321003;Parent=BBa_K1321342
BBa_K1321297	.	CDS	236	946	.	+	0	ID=BBa_K1321337;Name=BBa_K1321337;Parent=BBa_K1321342
BBa_K1321297	.	CDS	236	946	.	+	0	ID=annotation2417826;Name=sfGFP;Parent=BBa_K1321337
BBa_K1321297	.	sequence_feature	221	235	.	+	0	ID=BBa_K1319106;Name=BBa_K1319106;Parent=BBa_K1321357
BBa_K1321297	.	start_codon	227	229	.	+	0	ID=annotation2393317;Name=start;Parent=BBa_K1319106
BBa_K1321297	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1321357
BBa_K1321297	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1321297	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1321297	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1321297	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1321297	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1321297	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1321297	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1321297	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1321297	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1321297	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1321297	.	plasmid_vector	1288	4180	.	+	0	ID=BBa_K1321300;Name=BBa_K1321300
BBa_K1321297	.	sequence_feature	2485	3147	.	+	0	ID=annotation2412990;Name=Rep;Parent=BBa_K1321300
BBa_K1321297	.	CDS	1558	2217	.	+	0	ID=annotation2408619;Name=Chloramphenicol acetyltransferase;Parent=BBa_K1321300
BBa_K1321297	.	sequence_feature	1288	1309	.	+	0	ID=annotation2408497;Name=Biobrick suffix;Parent=BBa_K1321300
BBa_K1321297	.	sequence_feature	4159	4180	.	+	0	ID=annotation2408614;Name=Biobrick prefix;Parent=BBa_K1321300
BBa_K1321297	.	sequence_feature	2241	2476	.	+	0	ID=annotation2412975;Name=OriT;Parent=BBa_K1321300
>BBa_K1321297
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggccg
taaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttcc
gtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccgg
taccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagca
tgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaa
acgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacg
gcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatgg
cattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacact
ccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacg
agaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaac
cggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaag
aacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtgga
gctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcac
cgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgc
acggtcggcggcagctactagagtactagtagcggccgctgcagcttggactcctgttgatagatccagtaatgacctc
agaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaat
aattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagat
cactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggata
taccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataac
cagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattc
acattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatag
tgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttc
cggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattg
agaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaactt
cttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcat
catgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcgggg
cgtaatttgacttttgtccttttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcc
tttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggca
ggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgg
gaatcctgctctgcgaggctggccgtaggccggccctaccggcgcggcagcgttacccgtgtcggcggctccaacggct
cgccatcgtccagaaaacacggctcatcgggcatcggcaggcgctgctgcccgcgccgttcccattcctccgtttcggt
caaggctggcaggtctggttccatgcccggaatgccgggctggctgggcggctcctcgccggggccggtcggtagttgc
tgctcgcccggatacagggtcgggatgcggcgcaggtcgccatgccccaacagcgattcgtcctggtcgtcgtgatcaa
ccaccacggcggcactgaacaccgacaggcgcaactggtcgcggggctggccccacgccacgcggtcattgaccacgta
ggccgacacggtgccggggccgttgagcttcacgacggagatccagcgctcggccaccaagtccttgactgcgtattgg
accgtccgcaaagaacgtccgatgagcttggaaagtgtcttctggctgaccaccacggcgttctggtggcccatctgcg
ccacgaggtgatgcagcagcattgccgccgtgggtttcctcgcaataagcccggcccacgcctcatgcgctttgcgttc
cgtttgcacccagtgaccgggcttgttcttggcttgaatgccgatttctctggactgcgtggccatgcttatctccatg
cggtaggggtgccgcacggttgcggcaccatgcgcaatcagctgcaacttttcggcagcgcgacaacaattatgcgttg
cgtaaaagtggcagtcaattacagattttctttaacctacgcaatgagctattgcggggggtgccgcaatgagctgttg
cgtaccccccttttttaagttgttgatttttaagtctttcgcatttcgccctatatctagttctttggtgcccaaagaa
gggcacccctgcggggttcccccacgccttcggcgcggctccccctccggcaaaaagtggcccctccggggcttgttga
tcgactgcgcggccttcggccttgcccaaggtggcgctgcccccttggaacccccgcactcgccgccgtgaggctcggg
gggcaggcgggcgggcttcgcccttcgactgcccccactcgcataggcttgggtcgttccaggcgcgtcaaggccaagc
cgctgcgcggtcgctgcgcgagccttgacccgccttccacttggtgtccaaccggcaagcgaagcgcgcaggccgcagg
ccggaggcttttccccagagaaaattaaaaaaattgatggggcaaggccgcaggccgcgcagttggagccggtgggtat
gtggtcgaaggctgggtagccggtgggcaatccctgtggtcaagctcgtgggcaggcgcagcctgtccatcagcttgtc
cagcagggttgtccacgggccgagcgaagcgagccagccggtggccgctcgcggccatcgtccacatatccacgggctg
gcaagggagcgcagcgaccgcgcagggcgaagcccggagagcaagcccgtagggggggcgcgcccagctgtctagggcg
gcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctt
tcgttttatttgatgcctttaattaaagcggataacaatttcacacaggagaattcgcggccgcttctagag