LOCUS BBa_K1321299 4520 bp DNA linear 17-May-2024 DEFINITION J23101-GFP in pSEVA321-Bb ACCESSION BBa_K1321299 VERSION BBa_K1321299.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23101 RBS 44..56 /label=RBS-3\Weak RBS 50..53 /label=RBS misc_feature 44..56 /label=BBa_B0032 RBS 44..56 /label=BBa_B0032 CDS 63..782 /label=GFP protein CDS 63..782 /label=BBa_E0040 stem_loop 802..845 /label=stem_loop misc_feature 791..870 /label=BBa_B0010 terminator 791..870 /label=BBa_B0010 stem_loop 886..905 /label=T7 TE misc_feature 912..912 /label=stop polyA_site 906..919 /label=polya misc_feature 879..919 /label=BBa_B0012 terminator 879..919 /label=BBa_B0012 misc_feature 1..919 /label=BBa_I20260 misc_feature 928..948 /label=Biobrick suffix misc_feature 4499..4520 /label=Biobrick prefix CDS 1198..1857 /label=Chloramphenicol acetyltransferase misc_feature 1881..2116 /label=OriT misc_feature 2175..3323 /label=Replication protein TrfA misc_feature 928..4520 /label=BBa_K1321301 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact 61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac 301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt 361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg 421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca 481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg 541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag 601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc 721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat 781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 901 gggcctttct gcgtttatat actagagtac tagtagcggc cgctgcagct tggactcctg 961 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc 1021 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttaaattgg 1081 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga 1141 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg 1201 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat 1261 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt 1321 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac 1381 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag 1441 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg 1501 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg 1561 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat 1621 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc 1681 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac 1741 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc 1801 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt 1861 gacttttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg 1921 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc 1981 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg 2041 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc 2101 tgcgaggctg gccgtaggcc ggccgcgatg caggtggctg ctgaaccccc agccggaact 2161 gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc 2221 accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc 2281 acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc 2341 tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac 2401 ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg 2461 atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc 2521 agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt 2581 aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg 2641 tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg 2701 tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg 2761 taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc 2821 gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct 2881 cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc 2941 tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc tgacctgttt tgccaggtcc 3001 tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac 3061 ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc 3121 gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc 3181 tggactatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt 3241 gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc 3301 ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgga attaattccc 3361 cggatcgatc cgtcgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca 3421 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg 3481 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag 3541 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat 3601 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtggctgcc atttttgggg 3661 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg 3721 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg 3781 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca 3841 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg 3901 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt 3961 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg 4021 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt 4081 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca 4141 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg 4201 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggcccta catggctctg 4261 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc cccgcagaaa aaaaggatct 4321 caagaagatc ctttgatctt ttctacggcg cgcccagctg tctagggcgg cggatttgtc 4381 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac 4441 tgagcctttc gttttatttg atgcctttaa ttaaagcgga taacaatttc acacaggaga 4501 attcgcggcc gcttctagag //