LOCUS BBa_K1321301 3593 bp DNA linear 22-Nov-2024 DEFINITION pSEVA321-Bb ACCESSION BBa_K1321301 VERSION BBa_K1321301.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick suffix misc_feature 3572..3593 /label=Biobrick prefix CDS 271..930 /label=Chloramphenicol acetyltransferase misc_feature 954..1189 /label=OriT misc_feature 1248..2396 /label=Replication protein TrfA ORIGIN 1 tactagtagc ggccgctgca gcttggactc ctgttgatag atccagtaat gacctcagaa 61 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat 121 ccaggggtcc ccaataatta cgatttaaat tggcgaaaat gagacgttga tcggcacgta 181 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 241 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 301 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 361 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 421 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 481 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 541 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 601 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 661 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 721 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 781 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 841 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 901 tactgcgatg agtggcaggg cggggcgtaa tttgactttt gtccttttcc gctgcataac 961 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 1021 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 1081 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 1141 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggccgcg 1201 atgcaggtgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa 1261 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg 1321 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg 1381 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac 1441 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg 1501 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc 1561 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg 1621 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc 1681 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag 1741 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt 1801 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg 1861 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac 1921 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata 1981 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc 2041 tgcttggctt cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc 2101 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg 2161 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc 2221 acgctgtcgc gctcgatctt ggccgtagct tgctggacta tcgagccgac ggactggaag 2281 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa 2341 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac 2401 cctccttgcg ggattgcccc ggaattaatt ccccggatcg atccgtcgat cttgatcccc 2461 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa 2521 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc 2581 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt 2641 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg 2701 gactggcttt ctacgtggct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca 2761 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca 2821 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt 2881 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg 2941 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc 3001 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca 3061 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc 3121 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc 3181 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg 3241 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg 3301 tatccacaac gccggcggcc ctacatggct ctgctgtagt gagtgggttg cgctccggca 3361 gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 3421 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa 3481 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt 3541 taattaaagc ggataacaat ttcacacagg agaattcgcg gccgcttcta gag //