LOCUS BBa_K1321307 5045 bp DNA linear 28-Nov-2024 DEFINITION pSEVA351-Bb ACCESSION BBa_K1321307 VERSION BBa_K1321307.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick suffix CDS 271..931 /label=Chloramphenicol acetyltransferase misc_feature 954..1189 /label=OriT misc_feature 1191..1196 /label=NgoMIV RE site misc_feature 1198..2875 /label=RepC,A (replication proteins) CDS 3386..4357 /label=RepB misc_feature 4382..4411 /label=PlacUV5 misc_feature 5024..5045 /label=Biobrick prefix ORIGIN 1 tactagtagc ggccgctgca gcttggactc ctgttgatag atccagtaat gacctcagaa 61 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat 121 ccaggggtcc ccaataatta cgatttaaat tggcgaaaat gagacgttga tcggcacgta 181 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 241 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 301 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 361 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 421 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 481 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 541 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 601 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 661 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 721 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 781 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 841 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 901 tactgcgatg agtggcaggg cggggcgtaa tttgactttt gtccttttcc gctgcataac 961 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 1021 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 1081 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 1141 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcctca 1201 gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca 1261 gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt 1321 cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc 1381 ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct 1441 gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc 1501 ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta 1561 ctccgacagc agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt 1621 ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc 1681 cccgatttcc tttgccagcg cccgatagct acctttgacc acatggcatt cagcggtgac 1741 ggcctcccac ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc 1801 cgggccaagc actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag 1861 atcatcagcg cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc 1921 atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc 1981 gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg 2041 cttcaccacg gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca 2101 ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc 2161 tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct 2221 cggcgctggt catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc 2281 tgccccggct ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat 2341 ggtgcaggaa cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga 2401 cctgggccat ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca 2461 gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca 2521 tgatgttggg caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc 2581 gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg 2641 ggtcttcggc gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat 2701 ccggcccgcc tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac 2761 cgggcgacac cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg 2821 gtggcggcgc tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt 2881 gcctcctttg caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc 2941 cgctctgagt tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa 3001 cttgcgctga cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc 3061 gtcagggctg gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac 3121 cgagaaactt gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt 3181 caaggttaag gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg 3241 tataaccaaa gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc 3301 ctgaagcgct tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata 3361 acaggcgtga gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc 3421 ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca 3481 gacccatgac cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct 3541 ctgccagcgc tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc 3601 ggctggccag cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct 3661 tgaccagccc ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa 3721 gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg 3781 cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc 3841 ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct 3901 tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg 3961 cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt 4021 cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct 4081 cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca 4141 gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc 4201 gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct 4261 ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct 4321 gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gattcccaca 4381 cattatacga gccggaagca taaagtgtaa agcctagatc cgaaggatga gccgggctga 4441 atgatcgacc gagacaggcc ctgcggggct gcacacgcgc ccccaccctt cgggtagggg 4501 gaaaggccgc taaagcggct aaaagcgctc cagcgtattt ctgcggggtt tggtgtgggg 4561 tttagcgggc tttgcccgcc tttccccctg ccgcgcagcg gtggggcggt gtgtagccta 4621 gcgcagcgaa tagaccagct atccggcctc tggccgggca tattgggcaa gggcagcagc 4681 gccccacaag ggcgctgata accgcgccta gtggattatt cttagataat catggatgga 4741 tttttccaac accccgccag cccccgcccc tgctgggttt gcaggtttgg gggcgtgaca 4801 gttattgcag gggttcgtga cagttattgc aggggggcgt gacagttatt gcaggggttc 4861 gtgacagtta gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg 4921 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt 4981 atttgatgcc tttaattaaa gcggataaca atttcacaca ggagaattcg cggccgcttc 5041 tagag //