LOCUS BBa_K1321308 4688 bp DNA linear 14-Nov-2024 DEFINITION pBla-Vhb-122-Bb ACCESSION BBa_K1321308 VERSION BBa_K1321308.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick suffix CDS 480..1154 /label=Replication protein misc_feature 1680..2495 /label=KanR misc_feature 3393..4416 /label=Mobilization protein (contains a 4bp deletion) misc_feature 4667..4688 /label=Biobrick prefix ORIGIN 1 tactagtagc ggccgctgca gtttttttaa ggcagttatt ggtgccctta aacgcctggt 61 gctacgcctg aataagtata ataagcggat gaatggcaga aattcgaaag caaattcgac 121 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc 181 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt 241 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag 301 agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg 361 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga 421 ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc 481 taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa 541 cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg 601 gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg 661 ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg 721 ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac 781 accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag 841 gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag 901 tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc 961 tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt 1021 gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt 1081 tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc 1141 atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc aatcagctgc 1201 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat 1261 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc 1321 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg 1381 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc 1441 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa 1501 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg acctacaggg 1561 ggggggggga aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa 1621 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 1681 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg 1741 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 1801 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 1861 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 1921 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 1981 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 2041 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 2101 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 2161 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 2221 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 2281 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 2341 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 2401 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 2461 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac 2521 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt 2581 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 2641 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 2701 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 2761 ttcacgaggc agacctcagc gccccccccc ccctgaaggt ctcggggggc aggcgggcgg 2821 gcttcgcctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc 2881 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc 2941 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga 3001 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta 3061 gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt 3121 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc 3181 gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga 3241 gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa gcaaagtcta 3301 gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct gcccccgtcg 3361 agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc atgcgatgca 3421 agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg 3481 agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg gccagcagca 3541 ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg 3601 tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg gccagccaag 3661 aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag tacggggcgg 3721 atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg accgcgttcg 3781 tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc aacaaagcgc 3841 agatgacccg cgaccagacc acgtttgcgg acgctgtggc cgatctaggg ctgcaacggg 3901 gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag gccctggagc 3961 ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc gcctatgcac 4021 cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg 4081 ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag gccgccgcag 4141 gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga ccgagacctt 4201 cgggagcgcc tgaagcccgt tctggacgcc ctggggccgt tgaatcggga tatgcaggcc 4261 aaggccgccg cgatcatcaa ggccgtgggc gaaaagctgc tgacggaaca gcgggaagtc 4321 cagcgccaga aacaggccca gcgccagcag gaacgcgggc gcgcacattt ccccgaaaag 4381 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 4441 tcacgaggcc ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat 4501 aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga 4561 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 4621 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaagaat tcgcggccgc 4681 ttctagag //