LOCUS BBa_K1321329 4769 bp DNA linear 22-Nov-2024 DEFINITION GFP-LVA under TetR control in pSEVA331-Bb ACCESSION BBa_K1321329 VERSION BBa_K1321329.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_K145201 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 RBS 954..966 /label=RBS-3\Weak RBS 960..963 /label=RBS misc_feature 954..966 /label=BBa_B0032 RBS 954..966 /label=BBa_B0032 misc_feature 973..975 /label=start misc_feature 1726..1728 /label=stop misc_feature 1729..1731 /label=stop misc_feature 1689..1725 /label=LVA tag CDS 976..1688 /label=cds misc_feature 973..1731 /label=BBa_K145015 stem_loop 1751..1794 /label=stem_loop misc_feature 1740..1819 /label=BBa_B0010 terminator 1740..1819 /label=BBa_B0010 stem_loop 1835..1854 /label=T7 TE misc_feature 1861..1861 /label=stop polyA_site 1855..1868 /label=polya misc_feature 1828..1868 /label=BBa_B0012 terminator 1828..1868 /label=BBa_B0012 misc_feature 892..1868 /label=BBa_K145205 misc_feature 1..1868 /label=BBa_K145280 misc_feature 1877..1898 /label=Biobrick suffix misc_feature 4748..4769 /label=Biobrick prefix CDS 2147..2806 /label=Chloramphenicol acetyltransferase misc_feature 2830..3065 /label=OriT misc_feature 3074..3736 /label=Rep misc_feature 1877..4769 /label=BBa_K1321300 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca 961 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 1021 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 1081 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 1141 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 1201 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 1261 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 1321 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 1381 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 1441 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 1501 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 1561 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 1621 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 1681 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1861 cgtttatata ctagagtact agtagcggcc gctgcagctt ggactcctgt tgatagatcc 1921 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt 1981 ttttattggt gagaatccag gggtccccaa taattacgat ttaaattggc gaaaatgaga 2041 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 2101 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 2161 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 2221 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 2281 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 2341 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg 2401 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 2461 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 2521 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 2581 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 2641 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 2701 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 2761 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg acttttgtcc 2821 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc 2881 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc 2941 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc 3001 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg 3061 ccgtaggccg gccctaccgg cgcggcagcg ttacccgtgt cggcggctcc aacggctcgc 3121 catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc 3181 attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc 3241 tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga 3301 tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca 3361 cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt 3421 cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc 3481 gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct 3541 tggaaagtgt cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt 3601 gatgcagcag cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg 3661 ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc 3721 tggactgcgt ggccatgctt atctccatgc ggtaggggtg ccgcacggtt gcggcaccat 3781 gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc 3841 agtcaattac agattttctt taacctacgc aatgagctat tgcggggggt gccgcaatga 3901 gctgttgcgt accccccttt tttaagttgt tgatttttaa gtctttcgca tttcgcccta 3961 tatctagttc tttggtgccc aaagaagggc acccctgcgg ggttccccca cgccttcggc 4021 gcggctcccc ctccggcaaa aagtggcccc tccggggctt gttgatcgac tgcgcggcct 4081 tcggccttgc ccaaggtggc gctgccccct tggaaccccc gcactcgccg ccgtgaggct 4141 cggggggcag gcgggcgggc ttcgcccttc gactgccccc actcgcatag gcttgggtcg 4201 ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg acccgccttc 4261 cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc ttttccccag 4321 agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag ccggtgggta 4381 tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc 4441 agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc gagccagccg 4501 gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg cagcgaccgc 4561 gcagggcgaa gcccggagag caagcccgta gggggggcgc gcccagctgt ctagggcggc 4621 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag 4681 tctttcgact gagcctttcg ttttatttga tgcctttaat taaagcggat aacaatttca 4741 cacaggagaa ttcgcggccg cttctagag //