LOCUS BBa_K1321330 4718 bp DNA linear 22-Nov-2024 DEFINITION TetR repressed RFP in pSEVA331-Bb ACCESSION BBa_K1321330 VERSION BBa_K1321330.1 FEATURES Location/Qualifiers promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=P(Cat) promoter 1..38 /label=BBa_I14033 misc_feature 51..54 /label=conserved RBS 47..58 /label=BBa_B0034 CDS 68..684 /label=tetR misc_feature 685..718 /label=SsrA misc_feature 725..749 /label=Help:Barcodes CDS 65..749 /label=BBa_C0040 stem_loop 769..812 /label=stem_loop misc_feature 758..837 /label=BBa_B0010 terminator 758..837 /label=BBa_B0010 stem_loop 853..872 /label=T7 TE misc_feature 879..879 /label=stop polyA_site 873..886 /label=polya misc_feature 846..886 /label=BBa_B0012 terminator 846..886 /label=BBa_B0012 misc_feature 895..913 /label=TetR 1 misc_feature 895..948 /label=BBa_R0040 promoter 914..919 /label=-35 misc_feature 920..938 /label=TetR 2 promoter 937..942 /label=-10 promoter 895..948 /label=BBa_R0040 misc_feature 961..964 /label=conserved RBS 957..968 /label=BBa_B0034 CDS 975..1649 /label=mrfp1 misc_feature 1656..1680 /label=Help:Barcodes CDS 975..1680 /label=BBa_E1010 stem_loop 1700..1743 /label=stem_loop misc_feature 1689..1768 /label=BBa_B0010 terminator 1689..1768 /label=BBa_B0010 stem_loop 1784..1803 /label=T7 TE misc_feature 1810..1810 /label=stop polyA_site 1804..1817 /label=polya misc_feature 1777..1817 /label=BBa_B0012 terminator 1777..1817 /label=BBa_B0012 terminator 1689..1817 /label=BBa_B0015 misc_feature 1..1817 /label=BBa_K577895 misc_feature 1826..1847 /label=Biobrick suffix misc_feature 4697..4718 /label=Biobrick prefix CDS 2096..2755 /label=Chloramphenicol acetyltransferase misc_feature 2779..3014 /label=OriT misc_feature 3023..3685 /label=Rep misc_feature 1826..4718 /label=BBa_K1321300 ORIGIN 1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata 61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga 121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc 181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat 241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt 301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt 361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt 421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca 481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga 541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca 601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa 661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta 721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta 901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag 961 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag 1021 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc 1081 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt 1141 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc 1201 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg 1261 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg 1321 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta 1381 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg 1441 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg 1501 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg 1561 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac 1621 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc 1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1801 gcctttctgc gtttatatac tagagtacta gtagcggccg ctgcagcttg gactcctgtt 1861 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg 1921 ccgggcgttt tttattggtg agaatccagg ggtccccaat aattacgatt taaattggcg 1981 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc 2041 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga 2101 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 2161 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 2221 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 2281 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct 2341 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 2401 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 2461 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 2521 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 2581 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 2641 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 2701 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga 2761 cttttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 2821 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 2881 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 2941 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 3001 cgaggctggc cgtaggccgg ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca 3061 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc 3121 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc 3181 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca 3241 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat 3301 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg 3361 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg 3421 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc 3481 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg 3541 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg 3601 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc 3661 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc cgcacggttg 3721 cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt 3781 aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg 3841 ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat 3901 ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac 3961 gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact 4021 gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc 4081 cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca ctcgcatagg 4141 cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga 4201 cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct 4261 tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc 4321 cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg 4381 ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg 4441 agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc 4501 agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggggggcgcg cccagctgtc 4561 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 4621 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt aaagcggata 4681 acaatttcac acaggagaat tcgcggccgc ttctagag //