LOCUS BBa_K1321332 3970 bp DNA linear 12-Jun-2024 DEFINITION promoter(lacI regulated)-RFP in pSEVA331-Bb ACCESSION BBa_K1321332 VERSION BBa_K1321332.1 FEATURES Location/Qualifiers misc_feature 1..200 /label=BBa_R0010 CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start misc_feature 213..216 /label=conserved misc_feature 209..220 /label=BBa_B0034 CDS 227..901 /label=mrfp1 misc_feature 908..932 /label=Help:Barcodes misc_feature 227..932 /label=BBa_E1010 misc_feature 941..1020 /label=BBa_B0010 stem_loop 952..995 /label=stem_loop misc_feature 1029..1069 /label=BBa_B0012 stem_loop 1036..1055 /label=T7 TE polyA_site 1056..1069 /label=polya misc_feature 1062..1062 /label=stop misc_feature 941..1069 /label=BBa_B0015 misc_feature 224..229 /label=Nde1 Restriction Site misc_feature 1..1069 /label=BBa_K1041000 misc_feature 1078..1099 /label=Biobrick suffix misc_feature 3949..3970 /label=Biobrick prefix CDS 1348..2007 /label=Chloramphenicol acetyltransferase misc_feature 2031..2266 /label=OriT misc_feature 2275..2937 /label=Rep misc_feature 1078..3970 /label=BBa_K1321300 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa taccatatgg cttcctccga 241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtac 1081 tagtagcggc cgctgcagct tggactcctg ttgatagatc cagtaatgac ctcagaactc 1141 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca 1201 ggggtcccca ataattacga tttaaattgg cgaaaatgag acgttgatcg gcacgtaaga 1261 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg 1321 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 1381 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 1441 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 1501 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 1561 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 1621 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 1681 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 1741 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 1801 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 1861 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 1921 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 1981 tgcgatgagt ggcagggcgg ggcgtaattt gacttttgtc cttttccgct gcataaccct 2041 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag 2101 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca 2161 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc 2221 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccctaccg 2281 gcgcggcagc gttacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg 2341 ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa 2401 ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg 2461 gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg 2521 ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga 2581 caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga 2641 cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt 2701 gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct 2761 gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc 2821 cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac 2881 ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct 2941 tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt 3001 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct 3061 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt 3121 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc 3181 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa 3241 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg 3301 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg 3361 cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc 3421 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc 3481 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga 3541 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta 3601 gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt 3661 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc 3721 gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga 3781 gcaagcccgt agggggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga 3841 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 3901 gttttatttg atgcctttaa ttaaagcgga taacaatttc acacaggaga attcgcggcc 3961 gcttctagag //