LOCUS       BBa_K1321332            3970 bp    DNA     linear       22-Nov-2024
DEFINITION  promoter(lacI regulated)-RFP in pSEVA331-Bb
ACCESSION   BBa_K1321332
VERSION     BBa_K1321332.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    213..216
                     /label=conserved
     misc_feature    209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     misc_feature    227..932
                     /label=BBa_E1010
     misc_feature    941..1020
                     /label=BBa_B0010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    1029..1069
                     /label=BBa_B0012
     stem_loop       1036..1055
                     /label=T7 TE
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1062..1062
                     /label=stop
     misc_feature    941..1069
                     /label=BBa_B0015
     misc_feature    224..229
                     /label=Nde1 Restriction Site
     misc_feature    1..1069
                     /label=BBa_K1041000
     misc_feature    1078..1099
                     /label=Biobrick suffix
     misc_feature    3949..3970
                     /label=Biobrick prefix
     CDS             1348..2007
                     /label=Chloramphenicol acetyltransferase
     misc_feature    2031..2266
                     /label=OriT
     misc_feature    2275..2937
                     /label=Rep
     misc_feature    1078..3970
                     /label=BBa_K1321300
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa taccatatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtac
     1081 tagtagcggc cgctgcagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     1141 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     1201 ggggtcccca ataattacga tttaaattgg cgaaaatgag acgttgatcg gcacgtaaga
     1261 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     1321 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     1381 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     1441 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     1501 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1561 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1621 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1681 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1741 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1801 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1861 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1921 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1981 tgcgatgagt ggcagggcgg ggcgtaattt gacttttgtc cttttccgct gcataaccct
     2041 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2101 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2161 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2221 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccctaccg
     2281 gcgcggcagc gttacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg
     2341 ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa
     2401 ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg
     2461 gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg
     2521 ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga
     2581 caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga
     2641 cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt
     2701 gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct
     2761 gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc
     2821 cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac
     2881 ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct
     2941 tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt
     3001 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct
     3061 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt
     3121 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc
     3181 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa
     3241 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg
     3301 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg
     3361 cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc
     3421 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc
     3481 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga
     3541 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta
     3601 gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt
     3661 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc
     3721 gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga
     3781 gcaagcccgt agggggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     3841 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     3901 gttttatttg atgcctttaa ttaaagcgga taacaatttc acacaggaga attcgcggcc
     3961 gcttctagag
//