##gff-version 3
##sequence-region BBa_K1321332 1 3970
BBa_K1321332	.	plasmid_vector	1078	3970	.	+	0	ID=BBa_K1321300;Name=BBa_K1321300
BBa_K1321332	.	sequence_feature	2275	2937	.	+	0	ID=annotation2412990;Name=Rep;Parent=BBa_K1321300
BBa_K1321332	.	sequence_feature	2031	2266	.	+	0	ID=annotation2412975;Name=OriT;Parent=BBa_K1321300
BBa_K1321332	.	sequence_feature	1078	1099	.	+	0	ID=annotation2408497;Name=Biobrick suffix;Parent=BBa_K1321300
BBa_K1321332	.	sequence_feature	3949	3970	.	+	0	ID=annotation2408614;Name=Biobrick prefix;Parent=BBa_K1321300
BBa_K1321332	.	CDS	1348	2007	.	+	0	ID=annotation2408619;Name=Chloramphenicol acetyltransferase;Parent=BBa_K1321300
BBa_K1321332	.	engineered_region	1	1069	.	+	0	ID=BBa_K1041000;Name=BBa_K1041000
BBa_K1321332	.	engineered_region	1	200	.	+	0	ID=annotation2331500;Name=BBa_R0010;Parent=BBa_K1041000
BBa_K1321332	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2331502;Name=CAP binding site;Parent=BBa_K1041000
BBa_K1321332	.	engineered_region	1029	1069	.	+	0	ID=annotation2331514;Name=BBa_B0012;Parent=BBa_K1041000
BBa_K1321332	.	sequence_feature	224	229	.	+	0	ID=annotation2331523;Name=Nde1 Restriction Site;Parent=BBa_K1041000
BBa_K1321332	.	promoter	137	142	.	+	0	ID=annotation2331503;Name=-35;Parent=BBa_K1041000
BBa_K1321332	.	engineered_tag	908	932	.	+	0	ID=annotation2331510;Name=Help:Barcodes;Parent=BBa_K1041000
BBa_K1321332	.	promoter	161	166	.	+	0	ID=annotation2331504;Name=-10;Parent=BBa_K1041000
BBa_K1321332	.	engineered_region	209	220	.	+	0	ID=annotation2331508;Name=BBa_B0034;Parent=BBa_K1041000
BBa_K1321332	.	polyA_site	1056	1069	.	+	0	ID=annotation2331516;Name=polya;Parent=BBa_K1041000
BBa_K1321332	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2331505;Name=LacI binding site;Parent=BBa_K1041000
BBa_K1321332	.	CDS	1	88	.	+	0	ID=annotation2331501;Name=end of LacI coding region (inactive);Parent=BBa_K1041000
BBa_K1321332	.	engineered_region	941	1020	.	+	0	ID=annotation2331512;Name=BBa_B0010;Parent=BBa_K1041000
BBa_K1321332	.	CDS	227	901	.	+	0	ID=annotation2331509;Name=mrfp1;Parent=BBa_K1041000
BBa_K1321332	.	stem_loop	1036	1055	.	+	0	ID=annotation2331515;Name=T7 TE;Parent=BBa_K1041000
BBa_K1321332	.	conserved_region	213	216	.	+	0	ID=annotation2331507;Name=conserved;Parent=BBa_K1041000
BBa_K1321332	.	stem_loop	952	995	.	+	0	ID=annotation2331513;Name=stem_loop;Parent=BBa_K1041000
BBa_K1321332	.	start_codon	173	173	.	+	0	ID=annotation2331506;Name=start;Parent=BBa_K1041000
BBa_K1321332	.	stop_codon	1062	1062	.	+	0	ID=annotation2331517;Name=stop;Parent=BBa_K1041000
BBa_K1321332	.	engineered_region	227	932	.	+	0	ID=annotation2331511;Name=BBa_E1010;Parent=BBa_K1041000
BBa_K1321332	.	engineered_region	941	1069	.	+	0	ID=annotation2331518;Name=BBa_B0015;Parent=BBa_K1041000
>BBa_K1321332
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaataccatatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtagcggccgctgcagcttggact
cctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgtttttt
attggtgagaatccaggggtccccaataattacgatttaaattggcgaaaatgagacgttgatcggcacgtaagaggtt
ccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagc
taaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcattt
cagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaata
agcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaa
agacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcg
ctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacc
tggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttga
tttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtg
ctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaac
agtactgcgatgagtggcagggcggggcgtaatttgacttttgtccttttccgctgcataaccctgcttcggggtcatt
atagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttcctt
ggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtccc
ttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgtaggccggccctaccggcgcggcagcg
ttacccgtgtcggcggctccaacggctcgccatcgtccagaaaacacggctcatcgggcatcggcaggcgctgctgccc
gcgccgttcccattcctccgtttcggtcaaggctggcaggtctggttccatgcccggaatgccgggctggctgggcggc
tcctcgccggggccggtcggtagttgctgctcgcccggatacagggtcgggatgcggcgcaggtcgccatgccccaaca
gcgattcgtcctggtcgtcgtgatcaaccaccacggcggcactgaacaccgacaggcgcaactggtcgcggggctggcc
ccacgccacgcggtcattgaccacgtaggccgacacggtgccggggccgttgagcttcacgacggagatccagcgctcg
gccaccaagtccttgactgcgtattggaccgtccgcaaagaacgtccgatgagcttggaaagtgtcttctggctgacca
ccacggcgttctggtggcccatctgcgccacgaggtgatgcagcagcattgccgccgtgggtttcctcgcaataagccc
ggcccacgcctcatgcgctttgcgttccgtttgcacccagtgaccgggcttgttcttggcttgaatgccgatttctctg
gactgcgtggccatgcttatctccatgcggtaggggtgccgcacggttgcggcaccatgcgcaatcagctgcaactttt
cggcagcgcgacaacaattatgcgttgcgtaaaagtggcagtcaattacagattttctttaacctacgcaatgagctat
tgcggggggtgccgcaatgagctgttgcgtaccccccttttttaagttgttgatttttaagtctttcgcatttcgccct
atatctagttctttggtgcccaaagaagggcacccctgcggggttcccccacgccttcggcgcggctccccctccggca
aaaagtggcccctccggggcttgttgatcgactgcgcggccttcggccttgcccaaggtggcgctgcccccttggaacc
cccgcactcgccgccgtgaggctcggggggcaggcgggcgggcttcgcccttcgactgcccccactcgcataggcttgg
gtcgttccaggcgcgtcaaggccaagccgctgcgcggtcgctgcgcgagccttgacccgccttccacttggtgtccaac
cggcaagcgaagcgcgcaggccgcaggccggaggcttttccccagagaaaattaaaaaaattgatggggcaaggccgca
ggccgcgcagttggagccggtgggtatgtggtcgaaggctgggtagccggtgggcaatccctgtggtcaagctcgtggg
caggcgcagcctgtccatcagcttgtccagcagggttgtccacgggccgagcgaagcgagccagccggtggccgctcgc
ggccatcgtccacatatccacgggctggcaagggagcgcagcgaccgcgcagggcgaagcccggagagcaagcccgtag
ggggggcgcgcccagctgtctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacg
aaaggcccagtctttcgactgagcctttcgttttatttgatgcctttaattaaagcggataacaatttcacacaggaga
attcgcggccgcttctagag