BBa_K1321334 1 BBa_K1321334 pSB-AcsAB 2014-10-07T11:00:00Z 2015-05-08T01:09:53Z The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3. This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus. false false _1696_ 0 21121 9 It's complicated true The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully. false Laura de Arroyo Garcia annotation2428058 1 RBS - AcsAB range2428058 1 28 51 annotation2428734 1 Start Codon range2428734 1 68 71 annotation2428733 1 Double Stop Codon range2428733 1 4679 4684 annotation2428059 1 AcsAB Coding Sequence range2428059 1 68 4678 BBa_K1321334_sequence 1 gttttacagcaaagaggagaaattgtttatgccggaagttcgtagcagcacccagagcgaaagcggtatgagccagtggatgggtaaaattctgagcattcgtggtgcaggtctgattattggtgtttttggtctgtgtgcactgattgcagcaaccagcgttaccctgcctccggaacagcaactgattgttgcatttgtttgcgtggtgatcttttttatcgttggtcataaaccgagccgtcgtagccagatttttctggaagttctgagcggtctggttagcctgcgttatctgacctggcgtctgaccgaaaccctgagctttgatacctggctgcaaggtctgctgggcaccatgctgctggttgcagaactgtatgccctgatgatgctgtttctgagctattttcagaccattgcaccgctgcatcgtgctccgctgccgctgcctccgaatccggatgaatggccgaccgttgatatttttgttccgacctataatgaggaactgagcattgttcgcctgaccgttctgggtagcctgggtattgattggccaccggaaaaagttcgtgttcatattctggatgatggtcgtcgtccggaatttgcagcatttgccgcagaatgtggtgcaaactatattgcacgtccgaccaatgaacatgcaaaagcaggtaatctgaattatgccattggtcataccgatggcgattacattctgatctttgattgtgatcatgtgccgacccgtgcatttctgcaactgacaatgggttggatggttgaagatccgaaaattgcgctgatgcagacaccgcatcacttttatagtccggatccgtttcagcgtaatctgagcgcaggttatcgtacccctccggaaggtaacctgttttatggtgttgttcaggatggcaacgatttttgggatgcaacctttttttgtggtagctgtgcaattctgcgtcgtaccgcaattgagcagattggtggttttgcaacccagaccgttaccgaagatgcacataccgcactgaaaatgcagcgcctgggttggagcaccgcatatctgcgtattccgctggcaggcggtctggcaaccgaacgtctgattctgcatattggtcagcgtgttcgttgggcacgtggtatgctgcaaatctttcgtattgataatccgctgtttggtcgtggtctgagctggggtcagcgtctgtgttatctgagtgcaatgaccagctttctgtttgcagttccgcgtgttatttttctgagcagtccgctggcgtttctgttttttggtcagaacattattgcagcatcaccgctggcactgctggcatatgcaattccgcatatgtttcatgcagttggcaccgcaagcaaaattaacaaaggttggcgttatagcttttggagcgaagtttatgaaaccacaatggcactgtttctggttcgtgttaccattgtgaccctgctgagcccgagccgtggcaaatttaacgttaccgataaaggtggcctgctggaaaaaggttattttgatctgggtgcagtgtatccgaatattatcctgggcctgattatgtttggtggtctggcacgcggtgtttatgaactgagttttggtcatctggatcagattgcagaacgtgcctatctgctgaatagcgcatgggcaatgctgagcctgatcattattctggcagcaattgcagttggtcgtgaaacccagcagaaacgtaatagccatcgtattccggcaaccattccggttgaagttgcaaatgcagatggtagcattattgttaccggtgtgaccgaagatctgtccatgggtggtgcagcagttaaaatgagctggcctgcaaaactgagtggtccgacaccggtttatattcgtaccgtgctggatggcgaagaactgatcctgcctgcacgtattattcgtgccggtaatggtcgcggtatctttatttggaccatcgataatctgcaacaagagtttagcgttattcgtctggtttttggccgtgcagatgcatgggttgattggggtaactataaagcagatcgtccgctgctgagtctgatggatatggtgctgagcgttaaaggtctgtttcgtagctcaggtgatattgttcatcgtagtagcccgaccaaaccgagtgcaggtaatgcactgagtgatgatacaaataatccgagccgcaaagaacgtgttctgaaaggcaccgttaaaatggttagtctgctggccctgctgacctttgcaagcagcgcacaggcagcaagcgctccgcgtgcagttgcagccaaagcaccggcacatcagccggaagcaagcgatctgccaccgctgcctgccctgctgcctgcaacctcaggtgcagcccaggcaggtagtggtgatgccggtgcaaacggtccgggtagtccgaccggtcagcctctggcagccgatagcgcagatgccctggttgaaaatgcggaaaataccagcgataccgcaaccgttcataactataccctgaaagatctgggagcagcaggtagtattaccatgcgtggcctggcacctctgcaaggtattgaatttggtattccgagcgatcaactggttaccagcgcacgtctggttctgagtggtagcatgagcccgaatctgcgtccggaaaccaatagcgttaccatgaccctgaatgaacagtatatcggcaccctgcgtcctgatccagcacatccgacctttggtccgatgagttttgaaatcaatccgatctttttcgtgagcggtaatcgcctgaattttaactttgcgagcggtagcaaaggttgtagcgatattaccaatgataccctgtgggccaccattagccagaatagtcaactgcaaattaccaccattgctctgcctccgcgtcgtctgctgtctcgtctgccgcagccgttttatgataaaaatgttcgtcagcatgttaccgttccgatggttctggcacagacctatgatccgcagattctgaaaagcgcaggtattctggcgagctggtttggtaaacagaccgattttctgggtgttacctttccggtgagcagcaccattccgcagagtggtaatgccattctgattggtgttgccgatgaactgccgaccagcctgggtcgtccgcaggttaatggtccggcagtgctggaactgcctaatccgagtgatgcaaatgccaccattctggtggttaccggtcgcgatcgtgatgaagttattaccgccagcaaaggtattgcgtttgcaagcgcaccactgccgacagatagccatatggatgttgcaccggttgatatcgcaccgcgtaaacctaatgatgcaccgagctttattgcaatggatcatccggttcgctttggtgatctggttaccgcaagtaaactgcaaggcaccggttttaccagcggtgttctgtcagttccgtttcgtattcctccggatctgtatacctggcgtaatcgtccgtataaaatgcaggttcgttttcgtagtccggcaggcgaagcaaaagatgttgaaaaaagccgtctggatgtgggcattaatgaagtttatctgcatagctatccgctgcgtgaaacacatggtctgattggcgcagtgctgcaaggcgttggactggcacgtccggcatcaggtatgcaggtacatgatctggatgttccgccttggaccgtgtttggtcaggatcaactgaatttctattttgatgcaatgccgctggctcgtggtatttgtcagagcggtgcagcaaataatgcatttcatctgggtctggatccggatagcaccattgattttagccgtgcacatcatattgcacagatgccgaatctggcatatatggcaaccgttggttttccgtttaccacctatgccgatctgagtcagaccgcagttgttctgcctgaacatccgaatgcagccaccgttggtgcatatctggatctgatgggttttatgggtgccgcaacctggtatccggttgccggtgttgatattgtgagtgccgatcatgttagtgatgttgcggatcgtaatctgctggttattagcaccctggcaaccagtggtgaaattgcccctctgctgtcacgtagcagctatgaagttgcggatggacatctgcgtaccgttagccatgccagcgcactggataatgcaattaaagcagtggatgatccgctgaccgcatttcgtgatcgtgatagcaaaccgcaggatgttgatacaccgctgacaggtggtgttggtgcaatgattgaagcagaaagccctctgaccgcaggtcgcacagttctggcgctgctgtcaagtgatggcgcaggcctgaataatctgctgcaaatgctgggtgaacgtaaaaaacaggcaaatattcagggcgatctggttgttgcacatggtgaagatctgagcagctatcgtaccagtccggtgtataccattggtacactgccgctgtggctgtggcctgattggtatatgcataaccgtccggttcgtgtactgctggtaggactgctgggctgtattctgatcgttagcgtgctggcacgtgcactggcacgccatgcaacccgtcgttttaaacaactggaagatgaacgtcgcaaaagctgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z