LOCUS BBa_K1321336 5902 bp DNA linear 14-Nov-2024 DEFINITION AraC-pBAD-AcsAB ACCESSION BBa_K1321336 VERSION BBa_K1321336.1 FEATURES Location/Qualifiers CDS 1..1041 /label=AraC promoter 1042..1115 /label=PC promoter 1116..1210 /label=pBAD promoter 1..1210 /label=BBa_K1321333 RBS 1246..1269 /label=RBS - AcsAB CDS 1286..5896 /label=AcsAB Coding Sequence misc_feature 5897..5902 /label=Double Stop Codon misc_feature 1286..1289 /label=Start Codon misc_feature 1219..5902 /label=BBa_K1321334 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagaggt tttacagcaa agaggagaaa ttgtttatgc cggaagttcg 1261 tagcagcacc cagagcgaaa gcggtatgag ccagtggatg ggtaaaattc tgagcattcg 1321 tggtgcaggt ctgattattg gtgtttttgg tctgtgtgca ctgattgcag caaccagcgt 1381 taccctgcct ccggaacagc aactgattgt tgcatttgtt tgcgtggtga tcttttttat 1441 cgttggtcat aaaccgagcc gtcgtagcca gatttttctg gaagttctga gcggtctggt 1501 tagcctgcgt tatctgacct ggcgtctgac cgaaaccctg agctttgata cctggctgca 1561 aggtctgctg ggcaccatgc tgctggttgc agaactgtat gccctgatga tgctgtttct 1621 gagctatttt cagaccattg caccgctgca tcgtgctccg ctgccgctgc ctccgaatcc 1681 ggatgaatgg ccgaccgttg atatttttgt tccgacctat aatgaggaac tgagcattgt 1741 tcgcctgacc gttctgggta gcctgggtat tgattggcca ccggaaaaag ttcgtgttca 1801 tattctggat gatggtcgtc gtccggaatt tgcagcattt gccgcagaat gtggtgcaaa 1861 ctatattgca cgtccgacca atgaacatgc aaaagcaggt aatctgaatt atgccattgg 1921 tcataccgat ggcgattaca ttctgatctt tgattgtgat catgtgccga cccgtgcatt 1981 tctgcaactg acaatgggtt ggatggttga agatccgaaa attgcgctga tgcagacacc 2041 gcatcacttt tatagtccgg atccgtttca gcgtaatctg agcgcaggtt atcgtacccc 2101 tccggaaggt aacctgtttt atggtgttgt tcaggatggc aacgattttt gggatgcaac 2161 ctttttttgt ggtagctgtg caattctgcg tcgtaccgca attgagcaga ttggtggttt 2221 tgcaacccag accgttaccg aagatgcaca taccgcactg aaaatgcagc gcctgggttg 2281 gagcaccgca tatctgcgta ttccgctggc aggcggtctg gcaaccgaac gtctgattct 2341 gcatattggt cagcgtgttc gttgggcacg tggtatgctg caaatctttc gtattgataa 2401 tccgctgttt ggtcgtggtc tgagctgggg tcagcgtctg tgttatctga gtgcaatgac 2461 cagctttctg tttgcagttc cgcgtgttat ttttctgagc agtccgctgg cgtttctgtt 2521 ttttggtcag aacattattg cagcatcacc gctggcactg ctggcatatg caattccgca 2581 tatgtttcat gcagttggca ccgcaagcaa aattaacaaa ggttggcgtt atagcttttg 2641 gagcgaagtt tatgaaacca caatggcact gtttctggtt cgtgttacca ttgtgaccct 2701 gctgagcccg agccgtggca aatttaacgt taccgataaa ggtggcctgc tggaaaaagg 2761 ttattttgat ctgggtgcag tgtatccgaa tattatcctg ggcctgatta tgtttggtgg 2821 tctggcacgc ggtgtttatg aactgagttt tggtcatctg gatcagattg cagaacgtgc 2881 ctatctgctg aatagcgcat gggcaatgct gagcctgatc attattctgg cagcaattgc 2941 agttggtcgt gaaacccagc agaaacgtaa tagccatcgt attccggcaa ccattccggt 3001 tgaagttgca aatgcagatg gtagcattat tgttaccggt gtgaccgaag atctgtccat 3061 gggtggtgca gcagttaaaa tgagctggcc tgcaaaactg agtggtccga caccggttta 3121 tattcgtacc gtgctggatg gcgaagaact gatcctgcct gcacgtatta ttcgtgccgg 3181 taatggtcgc ggtatcttta tttggaccat cgataatctg caacaagagt ttagcgttat 3241 tcgtctggtt tttggccgtg cagatgcatg ggttgattgg ggtaactata aagcagatcg 3301 tccgctgctg agtctgatgg atatggtgct gagcgttaaa ggtctgtttc gtagctcagg 3361 tgatattgtt catcgtagta gcccgaccaa accgagtgca ggtaatgcac tgagtgatga 3421 tacaaataat ccgagccgca aagaacgtgt tctgaaaggc accgttaaaa tggttagtct 3481 gctggccctg ctgacctttg caagcagcgc acaggcagca agcgctccgc gtgcagttgc 3541 agccaaagca ccggcacatc agccggaagc aagcgatctg ccaccgctgc ctgccctgct 3601 gcctgcaacc tcaggtgcag cccaggcagg tagtggtgat gccggtgcaa acggtccggg 3661 tagtccgacc ggtcagcctc tggcagccga tagcgcagat gccctggttg aaaatgcgga 3721 aaataccagc gataccgcaa ccgttcataa ctataccctg aaagatctgg gagcagcagg 3781 tagtattacc atgcgtggcc tggcacctct gcaaggtatt gaatttggta ttccgagcga 3841 tcaactggtt accagcgcac gtctggttct gagtggtagc atgagcccga atctgcgtcc 3901 ggaaaccaat agcgttacca tgaccctgaa tgaacagtat atcggcaccc tgcgtcctga 3961 tccagcacat ccgacctttg gtccgatgag ttttgaaatc aatccgatct ttttcgtgag 4021 cggtaatcgc ctgaatttta actttgcgag cggtagcaaa ggttgtagcg atattaccaa 4081 tgataccctg tgggccacca ttagccagaa tagtcaactg caaattacca ccattgctct 4141 gcctccgcgt cgtctgctgt ctcgtctgcc gcagccgttt tatgataaaa atgttcgtca 4201 gcatgttacc gttccgatgg ttctggcaca gacctatgat ccgcagattc tgaaaagcgc 4261 aggtattctg gcgagctggt ttggtaaaca gaccgatttt ctgggtgtta cctttccggt 4321 gagcagcacc attccgcaga gtggtaatgc cattctgatt ggtgttgccg atgaactgcc 4381 gaccagcctg ggtcgtccgc aggttaatgg tccggcagtg ctggaactgc ctaatccgag 4441 tgatgcaaat gccaccattc tggtggttac cggtcgcgat cgtgatgaag ttattaccgc 4501 cagcaaaggt attgcgtttg caagcgcacc actgccgaca gatagccata tggatgttgc 4561 accggttgat atcgcaccgc gtaaacctaa tgatgcaccg agctttattg caatggatca 4621 tccggttcgc tttggtgatc tggttaccgc aagtaaactg caaggcaccg gttttaccag 4681 cggtgttctg tcagttccgt ttcgtattcc tccggatctg tatacctggc gtaatcgtcc 4741 gtataaaatg caggttcgtt ttcgtagtcc ggcaggcgaa gcaaaagatg ttgaaaaaag 4801 ccgtctggat gtgggcatta atgaagttta tctgcatagc tatccgctgc gtgaaacaca 4861 tggtctgatt ggcgcagtgc tgcaaggcgt tggactggca cgtccggcat caggtatgca 4921 ggtacatgat ctggatgttc cgccttggac cgtgtttggt caggatcaac tgaatttcta 4981 ttttgatgca atgccgctgg ctcgtggtat ttgtcagagc ggtgcagcaa ataatgcatt 5041 tcatctgggt ctggatccgg atagcaccat tgattttagc cgtgcacatc atattgcaca 5101 gatgccgaat ctggcatata tggcaaccgt tggttttccg tttaccacct atgccgatct 5161 gagtcagacc gcagttgttc tgcctgaaca tccgaatgca gccaccgttg gtgcatatct 5221 ggatctgatg ggttttatgg gtgccgcaac ctggtatccg gttgccggtg ttgatattgt 5281 gagtgccgat catgttagtg atgttgcgga tcgtaatctg ctggttatta gcaccctggc 5341 aaccagtggt gaaattgccc ctctgctgtc acgtagcagc tatgaagttg cggatggaca 5401 tctgcgtacc gttagccatg ccagcgcact ggataatgca attaaagcag tggatgatcc 5461 gctgaccgca tttcgtgatc gtgatagcaa accgcaggat gttgatacac cgctgacagg 5521 tggtgttggt gcaatgattg aagcagaaag ccctctgacc gcaggtcgca cagttctggc 5581 gctgctgtca agtgatggcg caggcctgaa taatctgctg caaatgctgg gtgaacgtaa 5641 aaaacaggca aatattcagg gcgatctggt tgttgcacat ggtgaagatc tgagcagcta 5701 tcgtaccagt ccggtgtata ccattggtac actgccgctg tggctgtggc ctgattggta 5761 tatgcataac cgtccggttc gtgtactgct ggtaggactg ctgggctgta ttctgatcgt 5821 tagcgtgctg gcacgtgcac tggcacgcca tgcaacccgt cgttttaaac aactggaaga 5881 tgaacgtcgc aaaagctgat aa //