BBa_K1321336 1 BBa_K1321336 AraC-pBAD-AcsAB 2014-10-07T11:00:00Z 2015-05-08T01:09:53Z The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3. This part is made up of the Arabinose-inducible promoter pBAD and the coding sequence of its transcriptional inhibitor/activator AraC, followed by the coding sequence of the A and B elements of the acs, cellulose-producing operon. false false _1696_ 0 21121 9 It's complicated false false Laura de Arroyo Garcia component2429265 1 BBa_K1321334 component2429260 1 BBa_K1321333 annotation2429265 1 BBa_K1321334 range2429265 1 1219 5902 annotation2429260 1 BBa_K1321333 range2429260 1 1 1210 BBa_K1321333 1 BBa_K1321333 pSB-AraC-pBAD 2014-10-07T11:00:00Z 2015-06-17T12:27:41Z This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter) This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream. false false _1696_ 4206 21121 9 In stock false false Laura de Arroyo Garcia annotation2429238 1 PC range2429238 1 1042 1115 annotation2429239 1 pBAD range2429239 1 1116 1210 annotation2429237 1 AraC range2429237 1 1 1041 BBa_K1321334 1 BBa_K1321334 pSB-AcsAB 2014-10-07T11:00:00Z 2015-05-08T01:09:53Z The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3. This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus. false false _1696_ 0 21121 9 It's complicated true The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully. false Laura de Arroyo Garcia annotation2428734 1 Start Codon range2428734 1 68 71 annotation2428059 1 AcsAB Coding Sequence range2428059 1 68 4678 annotation2428058 1 RBS - AcsAB range2428058 1 28 51 annotation2428733 1 Double Stop Codon range2428733 1 4679 4684 BBa_K1321336_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagaggttttacagcaaagaggagaaattgtttatgccggaagttcgtagcagcacccagagcgaaagcggtatgagccagtggatgggtaaaattctgagcattcgtggtgcaggtctgattattggtgtttttggtctgtgtgcactgattgcagcaaccagcgttaccctgcctccggaacagcaactgattgttgcatttgtttgcgtggtgatcttttttatcgttggtcataaaccgagccgtcgtagccagatttttctggaagttctgagcggtctggttagcctgcgttatctgacctggcgtctgaccgaaaccctgagctttgatacctggctgcaaggtctgctgggcaccatgctgctggttgcagaactgtatgccctgatgatgctgtttctgagctattttcagaccattgcaccgctgcatcgtgctccgctgccgctgcctccgaatccggatgaatggccgaccgttgatatttttgttccgacctataatgaggaactgagcattgttcgcctgaccgttctgggtagcctgggtattgattggccaccggaaaaagttcgtgttcatattctggatgatggtcgtcgtccggaatttgcagcatttgccgcagaatgtggtgcaaactatattgcacgtccgaccaatgaacatgcaaaagcaggtaatctgaattatgccattggtcataccgatggcgattacattctgatctttgattgtgatcatgtgccgacccgtgcatttctgcaactgacaatgggttggatggttgaagatccgaaaattgcgctgatgcagacaccgcatcacttttatagtccggatccgtttcagcgtaatctgagcgcaggttatcgtacccctccggaaggtaacctgttttatggtgttgttcaggatggcaacgatttttgggatgcaacctttttttgtggtagctgtgcaattctgcgtcgtaccgcaattgagcagattggtggttttgcaacccagaccgttaccgaagatgcacataccgcactgaaaatgcagcgcctgggttggagcaccgcatatctgcgtattccgctggcaggcggtctggcaaccgaacgtctgattctgcatattggtcagcgtgttcgttgggcacgtggtatgctgcaaatctttcgtattgataatccgctgtttggtcgtggtctgagctggggtcagcgtctgtgttatctgagtgcaatgaccagctttctgtttgcagttccgcgtgttatttttctgagcagtccgctggcgtttctgttttttggtcagaacattattgcagcatcaccgctggcactgctggcatatgcaattccgcatatgtttcatgcagttggcaccgcaagcaaaattaacaaaggttggcgttatagcttttggagcgaagtttatgaaaccacaatggcactgtttctggttcgtgttaccattgtgaccctgctgagcccgagccgtggcaaatttaacgttaccgataaaggtggcctgctggaaaaaggttattttgatctgggtgcagtgtatccgaatattatcctgggcctgattatgtttggtggtctggcacgcggtgtttatgaactgagttttggtcatctggatcagattgcagaacgtgcctatctgctgaatagcgcatgggcaatgctgagcctgatcattattctggcagcaattgcagttggtcgtgaaacccagcagaaacgtaatagccatcgtattccggcaaccattccggttgaagttgcaaatgcagatggtagcattattgttaccggtgtgaccgaagatctgtccatgggtggtgcagcagttaaaatgagctggcctgcaaaactgagtggtccgacaccggtttatattcgtaccgtgctggatggcgaagaactgatcctgcctgcacgtattattcgtgccggtaatggtcgcggtatctttatttggaccatcgataatctgcaacaagagtttagcgttattcgtctggtttttggccgtgcagatgcatgggttgattggggtaactataaagcagatcgtccgctgctgagtctgatggatatggtgctgagcgttaaaggtctgtttcgtagctcaggtgatattgttcatcgtagtagcccgaccaaaccgagtgcaggtaatgcactgagtgatgatacaaataatccgagccgcaaagaacgtgttctgaaaggcaccgttaaaatggttagtctgctggccctgctgacctttgcaagcagcgcacaggcagcaagcgctccgcgtgcagttgcagccaaagcaccggcacatcagccggaagcaagcgatctgccaccgctgcctgccctgctgcctgcaacctcaggtgcagcccaggcaggtagtggtgatgccggtgcaaacggtccgggtagtccgaccggtcagcctctggcagccgatagcgcagatgccctggttgaaaatgcggaaaataccagcgataccgcaaccgttcataactataccctgaaagatctgggagcagcaggtagtattaccatgcgtggcctggcacctctgcaaggtattgaatttggtattccgagcgatcaactggttaccagcgcacgtctggttctgagtggtagcatgagcccgaatctgcgtccggaaaccaatagcgttaccatgaccctgaatgaacagtatatcggcaccctgcgtcctgatccagcacatccgacctttggtccgatgagttttgaaatcaatccgatctttttcgtgagcggtaatcgcctgaattttaactttgcgagcggtagcaaaggttgtagcgatattaccaatgataccctgtgggccaccattagccagaatagtcaactgcaaattaccaccattgctctgcctccgcgtcgtctgctgtctcgtctgccgcagccgttttatgataaaaatgttcgtcagcatgttaccgttccgatggttctggcacagacctatgatccgcagattctgaaaagcgcaggtattctggcgagctggtttggtaaacagaccgattttctgggtgttacctttccggtgagcagcaccattccgcagagtggtaatgccattctgattggtgttgccgatgaactgccgaccagcctgggtcgtccgcaggttaatggtccggcagtgctggaactgcctaatccgagtgatgcaaatgccaccattctggtggttaccggtcgcgatcgtgatgaagttattaccgccagcaaaggtattgcgtttgcaagcgcaccactgccgacagatagccatatggatgttgcaccggttgatatcgcaccgcgtaaacctaatgatgcaccgagctttattgcaatggatcatccggttcgctttggtgatctggttaccgcaagtaaactgcaaggcaccggttttaccagcggtgttctgtcagttccgtttcgtattcctccggatctgtatacctggcgtaatcgtccgtataaaatgcaggttcgttttcgtagtccggcaggcgaagcaaaagatgttgaaaaaagccgtctggatgtgggcattaatgaagtttatctgcatagctatccgctgcgtgaaacacatggtctgattggcgcagtgctgcaaggcgttggactggcacgtccggcatcaggtatgcaggtacatgatctggatgttccgccttggaccgtgtttggtcaggatcaactgaatttctattttgatgcaatgccgctggctcgtggtatttgtcagagcggtgcagcaaataatgcatttcatctgggtctggatccggatagcaccattgattttagccgtgcacatcatattgcacagatgccgaatctggcatatatggcaaccgttggttttccgtttaccacctatgccgatctgagtcagaccgcagttgttctgcctgaacatccgaatgcagccaccgttggtgcatatctggatctgatgggttttatgggtgccgcaacctggtatccggttgccggtgttgatattgtgagtgccgatcatgttagtgatgttgcggatcgtaatctgctggttattagcaccctggcaaccagtggtgaaattgcccctctgctgtcacgtagcagctatgaagttgcggatggacatctgcgtaccgttagccatgccagcgcactggataatgcaattaaagcagtggatgatccgctgaccgcatttcgtgatcgtgatagcaaaccgcaggatgttgatacaccgctgacaggtggtgttggtgcaatgattgaagcagaaagccctctgaccgcaggtcgcacagttctggcgctgctgtcaagtgatggcgcaggcctgaataatctgctgcaaatgctgggtgaacgtaaaaaacaggcaaatattcagggcgatctggttgttgcacatggtgaagatctgagcagctatcgtaccagtccggtgtataccattggtacactgccgctgtggctgtggcctgattggtatatgcataaccgtccggttcgtgtactgctggtaggactgctgggctgtattctgatcgttagcgtgctggcacgtgcactggcacgccatgcaacccgtcgttttaaacaactggaagatgaacgtcgcaaaagctgataa BBa_K1321334_sequence 1 gttttacagcaaagaggagaaattgtttatgccggaagttcgtagcagcacccagagcgaaagcggtatgagccagtggatgggtaaaattctgagcattcgtggtgcaggtctgattattggtgtttttggtctgtgtgcactgattgcagcaaccagcgttaccctgcctccggaacagcaactgattgttgcatttgtttgcgtggtgatcttttttatcgttggtcataaaccgagccgtcgtagccagatttttctggaagttctgagcggtctggttagcctgcgttatctgacctggcgtctgaccgaaaccctgagctttgatacctggctgcaaggtctgctgggcaccatgctgctggttgcagaactgtatgccctgatgatgctgtttctgagctattttcagaccattgcaccgctgcatcgtgctccgctgccgctgcctccgaatccggatgaatggccgaccgttgatatttttgttccgacctataatgaggaactgagcattgttcgcctgaccgttctgggtagcctgggtattgattggccaccggaaaaagttcgtgttcatattctggatgatggtcgtcgtccggaatttgcagcatttgccgcagaatgtggtgcaaactatattgcacgtccgaccaatgaacatgcaaaagcaggtaatctgaattatgccattggtcataccgatggcgattacattctgatctttgattgtgatcatgtgccgacccgtgcatttctgcaactgacaatgggttggatggttgaagatccgaaaattgcgctgatgcagacaccgcatcacttttatagtccggatccgtttcagcgtaatctgagcgcaggttatcgtacccctccggaaggtaacctgttttatggtgttgttcaggatggcaacgatttttgggatgcaacctttttttgtggtagctgtgcaattctgcgtcgtaccgcaattgagcagattggtggttttgcaacccagaccgttaccgaagatgcacataccgcactgaaaatgcagcgcctgggttggagcaccgcatatctgcgtattccgctggcaggcggtctggcaaccgaacgtctgattctgcatattggtcagcgtgttcgttgggcacgtggtatgctgcaaatctttcgtattgataatccgctgtttggtcgtggtctgagctggggtcagcgtctgtgttatctgagtgcaatgaccagctttctgtttgcagttccgcgtgttatttttctgagcagtccgctggcgtttctgttttttggtcagaacattattgcagcatcaccgctggcactgctggcatatgcaattccgcatatgtttcatgcagttggcaccgcaagcaaaattaacaaaggttggcgttatagcttttggagcgaagtttatgaaaccacaatggcactgtttctggttcgtgttaccattgtgaccctgctgagcccgagccgtggcaaatttaacgttaccgataaaggtggcctgctggaaaaaggttattttgatctgggtgcagtgtatccgaatattatcctgggcctgattatgtttggtggtctggcacgcggtgtttatgaactgagttttggtcatctggatcagattgcagaacgtgcctatctgctgaatagcgcatgggcaatgctgagcctgatcattattctggcagcaattgcagttggtcgtgaaacccagcagaaacgtaatagccatcgtattccggcaaccattccggttgaagttgcaaatgcagatggtagcattattgttaccggtgtgaccgaagatctgtccatgggtggtgcagcagttaaaatgagctggcctgcaaaactgagtggtccgacaccggtttatattcgtaccgtgctggatggcgaagaactgatcctgcctgcacgtattattcgtgccggtaatggtcgcggtatctttatttggaccatcgataatctgcaacaagagtttagcgttattcgtctggtttttggccgtgcagatgcatgggttgattggggtaactataaagcagatcgtccgctgctgagtctgatggatatggtgctgagcgttaaaggtctgtttcgtagctcaggtgatattgttcatcgtagtagcccgaccaaaccgagtgcaggtaatgcactgagtgatgatacaaataatccgagccgcaaagaacgtgttctgaaaggcaccgttaaaatggttagtctgctggccctgctgacctttgcaagcagcgcacaggcagcaagcgctccgcgtgcagttgcagccaaagcaccggcacatcagccggaagcaagcgatctgccaccgctgcctgccctgctgcctgcaacctcaggtgcagcccaggcaggtagtggtgatgccggtgcaaacggtccgggtagtccgaccggtcagcctctggcagccgatagcgcagatgccctggttgaaaatgcggaaaataccagcgataccgcaaccgttcataactataccctgaaagatctgggagcagcaggtagtattaccatgcgtggcctggcacctctgcaaggtattgaatttggtattccgagcgatcaactggttaccagcgcacgtctggttctgagtggtagcatgagcccgaatctgcgtccggaaaccaatagcgttaccatgaccctgaatgaacagtatatcggcaccctgcgtcctgatccagcacatccgacctttggtccgatgagttttgaaatcaatccgatctttttcgtgagcggtaatcgcctgaattttaactttgcgagcggtagcaaaggttgtagcgatattaccaatgataccctgtgggccaccattagccagaatagtcaactgcaaattaccaccattgctctgcctccgcgtcgtctgctgtctcgtctgccgcagccgttttatgataaaaatgttcgtcagcatgttaccgttccgatggttctggcacagacctatgatccgcagattctgaaaagcgcaggtattctggcgagctggtttggtaaacagaccgattttctgggtgttacctttccggtgagcagcaccattccgcagagtggtaatgccattctgattggtgttgccgatgaactgccgaccagcctgggtcgtccgcaggttaatggtccggcagtgctggaactgcctaatccgagtgatgcaaatgccaccattctggtggttaccggtcgcgatcgtgatgaagttattaccgccagcaaaggtattgcgtttgcaagcgcaccactgccgacagatagccatatggatgttgcaccggttgatatcgcaccgcgtaaacctaatgatgcaccgagctttattgcaatggatcatccggttcgctttggtgatctggttaccgcaagtaaactgcaaggcaccggttttaccagcggtgttctgtcagttccgtttcgtattcctccggatctgtatacctggcgtaatcgtccgtataaaatgcaggttcgttttcgtagtccggcaggcgaagcaaaagatgttgaaaaaagccgtctggatgtgggcattaatgaagtttatctgcatagctatccgctgcgtgaaacacatggtctgattggcgcagtgctgcaaggcgttggactggcacgtccggcatcaggtatgcaggtacatgatctggatgttccgccttggaccgtgtttggtcaggatcaactgaatttctattttgatgcaatgccgctggctcgtggtatttgtcagagcggtgcagcaaataatgcatttcatctgggtctggatccggatagcaccattgattttagccgtgcacatcatattgcacagatgccgaatctggcatatatggcaaccgttggttttccgtttaccacctatgccgatctgagtcagaccgcagttgttctgcctgaacatccgaatgcagccaccgttggtgcatatctggatctgatgggttttatgggtgccgcaacctggtatccggttgccggtgttgatattgtgagtgccgatcatgttagtgatgttgcggatcgtaatctgctggttattagcaccctggcaaccagtggtgaaattgcccctctgctgtcacgtagcagctatgaagttgcggatggacatctgcgtaccgttagccatgccagcgcactggataatgcaattaaagcagtggatgatccgctgaccgcatttcgtgatcgtgatagcaaaccgcaggatgttgatacaccgctgacaggtggtgttggtgcaatgattgaagcagaaagccctctgaccgcaggtcgcacagttctggcgctgctgtcaagtgatggcgcaggcctgaataatctgctgcaaatgctgggtgaacgtaaaaaacaggcaaatattcagggcgatctggttgttgcacatggtgaagatctgagcagctatcgtaccagtccggtgtataccattggtacactgccgctgtggctgtggcctgattggtatatgcataaccgtccggttcgtgtactgctggtaggactgctgggctgtattctgatcgttagcgtgctggcacgtgcactggcacgccatgcaacccgtcgttttaaacaactggaagatgaacgtcgcaaaagctgataa BBa_K1321333_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z