BBa_K1321336
1
BBa_K1321336
AraC-pBAD-AcsAB
2014-10-07T11:00:00Z
2015-05-08T01:09:53Z
The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.
This part is made up of the Arabinose-inducible promoter pBAD and the coding sequence of its transcriptional inhibitor/activator AraC, followed by the coding sequence of the A and B elements of the acs, cellulose-producing operon.
false
false
_1696_
0
21121
9
It's complicated
false
false
Laura de Arroyo Garcia
component2429265
1
BBa_K1321334
component2429260
1
BBa_K1321333
annotation2429265
1
BBa_K1321334
range2429265
1
1219
5902
annotation2429260
1
BBa_K1321333
range2429260
1
1
1210
BBa_K1321333
1
BBa_K1321333
pSB-AraC-pBAD
2014-10-07T11:00:00Z
2015-06-17T12:27:41Z
This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
false
false
_1696_
4206
21121
9
In stock
false
false
Laura de Arroyo Garcia
annotation2429238
1
PC
range2429238
1
1042
1115
annotation2429239
1
pBAD
range2429239
1
1116
1210
annotation2429237
1
AraC
range2429237
1
1
1041
BBa_K1321334
1
BBa_K1321334
pSB-AcsAB
2014-10-07T11:00:00Z
2015-05-08T01:09:53Z
The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.
This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus.
false
false
_1696_
0
21121
9
It's complicated
true
The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully.
false
Laura de Arroyo Garcia
annotation2428734
1
Start Codon
range2428734
1
68
71
annotation2428059
1
AcsAB Coding Sequence
range2428059
1
68
4678
annotation2428058
1
RBS - AcsAB
range2428058
1
28
51
annotation2428733
1
Double Stop Codon
range2428733
1
4679
4684
BBa_K1321336_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagaggttttacagcaaagaggagaaattgtttatgccggaagttcgtagcagcacccagagcgaaagcggtatgagccagtggatgggtaaaattctgagcattcgtggtgcaggtctgattattggtgtttttggtctgtgtgcactgattgcagcaaccagcgttaccctgcctccggaacagcaactgattgttgcatttgtttgcgtggtgatcttttttatcgttggtcataaaccgagccgtcgtagccagatttttctggaagttctgagcggtctggttagcctgcgttatctgacctggcgtctgaccgaaaccctgagctttgatacctggctgcaaggtctgctgggcaccatgctgctggttgcagaactgtatgccctgatgatgctgtttctgagctattttcagaccattgcaccgctgcatcgtgctccgctgccgctgcctccgaatccggatgaatggccgaccgttgatatttttgttccgacctataatgaggaactgagcattgttcgcctgaccgttctgggtagcctgggtattgattggccaccggaaaaagttcgtgttcatattctggatgatggtcgtcgtccggaatttgcagcatttgccgcagaatgtggtgcaaactatattgcacgtccgaccaatgaacatgcaaaagcaggtaatctgaattatgccattggtcataccgatggcgattacattctgatctttgattgtgatcatgtgccgacccgtgcatttctgcaactgacaatgggttggatggttgaagatccgaaaattgcgctgatgcagacaccgcatcacttttatagtccggatccgtttcagcgtaatctgagcgcaggttatcgtacccctccggaaggtaacctgttttatggtgttgttcaggatggcaacgatttttgggatgcaacctttttttgtggtagctgtgcaattctgcgtcgtaccgcaattgagcagattggtggttttgcaacccagaccgttaccgaagatgcacataccgcactgaaaatgcagcgcctgggttggagcaccgcatatctgcgtattccgctggcaggcggtctggcaaccgaacgtctgattctgcatattggtcagcgtgttcgttgggcacgtggtatgctgcaaatctttcgtattgataatccgctgtttggtcgtggtctgagctggggtcagcgtctgtgttatctgagtgcaatgaccagctttctgtttgcagttccgcgtgttatttttctgagcagtccgctggcgtttctgttttttggtcagaacattattgcagcatcaccgctggcactgctggcatatgcaattccgcatatgtttcatgcagttggcaccgcaagcaaaattaacaaaggttggcgttatagcttttggagcgaagtttatgaaaccacaatggcactgtttctggttcgtgttaccattgtgaccctgctgagcccgagccgtggcaaatttaacgttaccgataaaggtggcctgctggaaaaaggttattttgatctgggtgcagtgtatccgaatattatcctgggcctgattatgtttggtggtctggcacgcggtgtttatgaactgagttttggtcatctggatcagattgcagaacgtgcctatctgctgaatagcgcatgggcaatgctgagcctgatcattattctggcagcaattgcagttggtcgtgaaacccagcagaaacgtaatagccatcgtattccggcaaccattccggttgaagttgcaaatgcagatggtagcattattgttaccggtgtgaccgaagatctgtccatgggtggtgcagcagttaaaatgagctggcctgcaaaactgagtggtccgacaccggtttatattcgtaccgtgctggatggcgaagaactgatcctgcctgcacgtattattcgtgccggtaatggtcgcggtatctttatttggaccatcgataatctgcaacaagagtttagcgttattcgtctggtttttggccgtgcagatgcatgggttgattggggtaactataaagcagatcgtccgctgctgagtctgatggatatggtgctgagcgttaaaggtctgtttcgtagctcaggtgatattgttcatcgtagtagcccgaccaaaccgagtgcaggtaatgcactgagtgatgatacaaataatccgagccgcaaagaacgtgttctgaaaggcaccgttaaaatggttagtctgctggccctgctgacctttgcaagcagcgcacaggcagcaagcgctccgcgtgcagttgcagccaaagcaccggcacatcagccggaagcaagcgatctgccaccgctgcctgccctgctgcctgcaacctcaggtgcagcccaggcaggtagtggtgatgccggtgcaaacggtccgggtagtccgaccggtcagcctctggcagccgatagcgcagatgccctggttgaaaatgcggaaaataccagcgataccgcaaccgttcataactataccctgaaagatctgggagcagcaggtagtattaccatgcgtggcctggcacctctgcaaggtattgaatttggtattccgagcgatcaactggttaccagcgcacgtctggttctgagtggtagcatgagcccgaatctgcgtccggaaaccaatagcgttaccatgaccctgaatgaacagtatatcggcaccctgcgtcctgatccagcacatccgacctttggtccgatgagttttgaaatcaatccgatctttttcgtgagcggtaatcgcctgaattttaactttgcgagcggtagcaaaggttgtagcgatattaccaatgataccctgtgggccaccattagccagaatagtcaactgcaaattaccaccattgctctgcctccgcgtcgtctgctgtctcgtctgccgcagccgttttatgataaaaatgttcgtcagcatgttaccgttccgatggttctggcacagacctatgatccgcagattctgaaaagcgcaggtattctggcgagctggtttggtaaacagaccgattttctgggtgttacctttccggtgagcagcaccattccgcagagtggtaatgccattctgattggtgttgccgatgaactgccgaccagcctgggtcgtccgcaggttaatggtccggcagtgctggaactgcctaatccgagtgatgcaaatgccaccattctggtggttaccggtcgcgatcgtgatgaagttattaccgccagcaaaggtattgcgtttgcaagcgcaccactgccgacagatagccatatggatgttgcaccggttgatatcgcaccgcgtaaacctaatgatgcaccgagctttattgcaatggatcatccggttcgctttggtgatctggttaccgcaagtaaactgcaaggcaccggttttaccagcggtgttctgtcagttccgtttcgtattcctccggatctgtatacctggcgtaatcgtccgtataaaatgcaggttcgttttcgtagtccggcaggcgaagcaaaagatgttgaaaaaagccgtctggatgtgggcattaatgaagtttatctgcatagctatccgctgcgtgaaacacatggtctgattggcgcagtgctgcaaggcgttggactggcacgtccggcatcaggtatgcaggtacatgatctggatgttccgccttggaccgtgtttggtcaggatcaactgaatttctattttgatgcaatgccgctggctcgtggtatttgtcagagcggtgcagcaaataatgcatttcatctgggtctggatccggatagcaccattgattttagccgtgcacatcatattgcacagatgccgaatctggcatatatggcaaccgttggttttccgtttaccacctatgccgatctgagtcagaccgcagttgttctgcctgaacatccgaatgcagccaccgttggtgcatatctggatctgatgggttttatgggtgccgcaacctggtatccggttgccggtgttgatattgtgagtgccgatcatgttagtgatgttgcggatcgtaatctgctggttattagcaccctggcaaccagtggtgaaattgcccctctgctgtcacgtagcagctatgaagttgcggatggacatctgcgtaccgttagccatgccagcgcactggataatgcaattaaagcagtggatgatccgctgaccgcatttcgtgatcgtgatagcaaaccgcaggatgttgatacaccgctgacaggtggtgttggtgcaatgattgaagcagaaagccctctgaccgcaggtcgcacagttctggcgctgctgtcaagtgatggcgcaggcctgaataatctgctgcaaatgctgggtgaacgtaaaaaacaggcaaatattcagggcgatctggttgttgcacatggtgaagatctgagcagctatcgtaccagtccggtgtataccattggtacactgccgctgtggctgtggcctgattggtatatgcataaccgtccggttcgtgtactgctggtaggactgctgggctgtattctgatcgttagcgtgctggcacgtgcactggcacgccatgcaacccgtcgttttaaacaactggaagatgaacgtcgcaaaagctgataa
BBa_K1321334_sequence
1
gttttacagcaaagaggagaaattgtttatgccggaagttcgtagcagcacccagagcgaaagcggtatgagccagtggatgggtaaaattctgagcattcgtggtgcaggtctgattattggtgtttttggtctgtgtgcactgattgcagcaaccagcgttaccctgcctccggaacagcaactgattgttgcatttgtttgcgtggtgatcttttttatcgttggtcataaaccgagccgtcgtagccagatttttctggaagttctgagcggtctggttagcctgcgttatctgacctggcgtctgaccgaaaccctgagctttgatacctggctgcaaggtctgctgggcaccatgctgctggttgcagaactgtatgccctgatgatgctgtttctgagctattttcagaccattgcaccgctgcatcgtgctccgctgccgctgcctccgaatccggatgaatggccgaccgttgatatttttgttccgacctataatgaggaactgagcattgttcgcctgaccgttctgggtagcctgggtattgattggccaccggaaaaagttcgtgttcatattctggatgatggtcgtcgtccggaatttgcagcatttgccgcagaatgtggtgcaaactatattgcacgtccgaccaatgaacatgcaaaagcaggtaatctgaattatgccattggtcataccgatggcgattacattctgatctttgattgtgatcatgtgccgacccgtgcatttctgcaactgacaatgggttggatggttgaagatccgaaaattgcgctgatgcagacaccgcatcacttttatagtccggatccgtttcagcgtaatctgagcgcaggttatcgtacccctccggaaggtaacctgttttatggtgttgttcaggatggcaacgatttttgggatgcaacctttttttgtggtagctgtgcaattctgcgtcgtaccgcaattgagcagattggtggttttgcaacccagaccgttaccgaagatgcacataccgcactgaaaatgcagcgcctgggttggagcaccgcatatctgcgtattccgctggcaggcggtctggcaaccgaacgtctgattctgcatattggtcagcgtgttcgttgggcacgtggtatgctgcaaatctttcgtattgataatccgctgtttggtcgtggtctgagctggggtcagcgtctgtgttatctgagtgcaatgaccagctttctgtttgcagttccgcgtgttatttttctgagcagtccgctggcgtttctgttttttggtcagaacattattgcagcatcaccgctggcactgctggcatatgcaattccgcatatgtttcatgcagttggcaccgcaagcaaaattaacaaaggttggcgttatagcttttggagcgaagtttatgaaaccacaatggcactgtttctggttcgtgttaccattgtgaccctgctgagcccgagccgtggcaaatttaacgttaccgataaaggtggcctgctggaaaaaggttattttgatctgggtgcagtgtatccgaatattatcctgggcctgattatgtttggtggtctggcacgcggtgtttatgaactgagttttggtcatctggatcagattgcagaacgtgcctatctgctgaatagcgcatgggcaatgctgagcctgatcattattctggcagcaattgcagttggtcgtgaaacccagcagaaacgtaatagccatcgtattccggcaaccattccggttgaagttgcaaatgcagatggtagcattattgttaccggtgtgaccgaagatctgtccatgggtggtgcagcagttaaaatgagctggcctgcaaaactgagtggtccgacaccggtttatattcgtaccgtgctggatggcgaagaactgatcctgcctgcacgtattattcgtgccggtaatggtcgcggtatctttatttggaccatcgataatctgcaacaagagtttagcgttattcgtctggtttttggccgtgcagatgcatgggttgattggggtaactataaagcagatcgtccgctgctgagtctgatggatatggtgctgagcgttaaaggtctgtttcgtagctcaggtgatattgttcatcgtagtagcccgaccaaaccgagtgcaggtaatgcactgagtgatgatacaaataatccgagccgcaaagaacgtgttctgaaaggcaccgttaaaatggttagtctgctggccctgctgacctttgcaagcagcgcacaggcagcaagcgctccgcgtgcagttgcagccaaagcaccggcacatcagccggaagcaagcgatctgccaccgctgcctgccctgctgcctgcaacctcaggtgcagcccaggcaggtagtggtgatgccggtgcaaacggtccgggtagtccgaccggtcagcctctggcagccgatagcgcagatgccctggttgaaaatgcggaaaataccagcgataccgcaaccgttcataactataccctgaaagatctgggagcagcaggtagtattaccatgcgtggcctggcacctctgcaaggtattgaatttggtattccgagcgatcaactggttaccagcgcacgtctggttctgagtggtagcatgagcccgaatctgcgtccggaaaccaatagcgttaccatgaccctgaatgaacagtatatcggcaccctgcgtcctgatccagcacatccgacctttggtccgatgagttttgaaatcaatccgatctttttcgtgagcggtaatcgcctgaattttaactttgcgagcggtagcaaaggttgtagcgatattaccaatgataccctgtgggccaccattagccagaatagtcaactgcaaattaccaccattgctctgcctccgcgtcgtctgctgtctcgtctgccgcagccgttttatgataaaaatgttcgtcagcatgttaccgttccgatggttctggcacagacctatgatccgcagattctgaaaagcgcaggtattctggcgagctggtttggtaaacagaccgattttctgggtgttacctttccggtgagcagcaccattccgcagagtggtaatgccattctgattggtgttgccgatgaactgccgaccagcctgggtcgtccgcaggttaatggtccggcagtgctggaactgcctaatccgagtgatgcaaatgccaccattctggtggttaccggtcgcgatcgtgatgaagttattaccgccagcaaaggtattgcgtttgcaagcgcaccactgccgacagatagccatatggatgttgcaccggttgatatcgcaccgcgtaaacctaatgatgcaccgagctttattgcaatggatcatccggttcgctttggtgatctggttaccgcaagtaaactgcaaggcaccggttttaccagcggtgttctgtcagttccgtttcgtattcctccggatctgtatacctggcgtaatcgtccgtataaaatgcaggttcgttttcgtagtccggcaggcgaagcaaaagatgttgaaaaaagccgtctggatgtgggcattaatgaagtttatctgcatagctatccgctgcgtgaaacacatggtctgattggcgcagtgctgcaaggcgttggactggcacgtccggcatcaggtatgcaggtacatgatctggatgttccgccttggaccgtgtttggtcaggatcaactgaatttctattttgatgcaatgccgctggctcgtggtatttgtcagagcggtgcagcaaataatgcatttcatctgggtctggatccggatagcaccattgattttagccgtgcacatcatattgcacagatgccgaatctggcatatatggcaaccgttggttttccgtttaccacctatgccgatctgagtcagaccgcagttgttctgcctgaacatccgaatgcagccaccgttggtgcatatctggatctgatgggttttatgggtgccgcaacctggtatccggttgccggtgttgatattgtgagtgccgatcatgttagtgatgttgcggatcgtaatctgctggttattagcaccctggcaaccagtggtgaaattgcccctctgctgtcacgtagcagctatgaagttgcggatggacatctgcgtaccgttagccatgccagcgcactggataatgcaattaaagcagtggatgatccgctgaccgcatttcgtgatcgtgatagcaaaccgcaggatgttgatacaccgctgacaggtggtgttggtgcaatgattgaagcagaaagccctctgaccgcaggtcgcacagttctggcgctgctgtcaagtgatggcgcaggcctgaataatctgctgcaaatgctgggtgaacgtaaaaaacaggcaaatattcagggcgatctggttgttgcacatggtgaagatctgagcagctatcgtaccagtccggtgtataccattggtacactgccgctgtggctgtggcctgattggtatatgcataaccgtccggttcgtgtactgctggtaggactgctgggctgtattctgatcgttagcgtgctggcacgtgcactggcacgccatgcaacccgtcgttttaaacaactggaagatgaacgtcgcaaaagctgataa
BBa_K1321333_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z