LOCUS       BBa_K1321354            1075 bp    DNA     linear       23-May-2024
DEFINITION  sfGFP fused to CBDclos driven by T7
ACCESSION   BBa_K1321354
VERSION     BBa_K1321354.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             1..43
                     /label=BBa_K1321338
     misc_feature    50..52
                     /label=start
     misc_feature    44..58
                     /label=BBa_K1319106
     CDS             59..769
                     /label=sfGFP
     CDS             59..769
                     /label=BBa_K1321337
     misc_feature    770..775
                     /label=Scar 25
     misc_feature    770..775
                     /label=BBa_B0105
     CDS             776..1075
                     /label=BBa_K1321002
     CDS             59..1075
                     /label=BBa_K1321341
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggccggccg
       61 taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
      121 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
      181 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
      241 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
      301 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
      361 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
      421 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
      481 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
      541 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
      601 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
      661 cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
      721 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaaa ccggctcatc
      781 aatgtcagtt gaattttaca actctaacaa atcagcacaa acaaactcaa ttacaccaat
      841 aatcaaaatt actaacacgt ctgacagtga tttaaattta aatgacgtaa aagttagata
      901 ttattacaca agtgatggta cacaaggaca aactttctgg tgtgaccatg ctggtgcatt
      961 attaggaaat agctatgttg ataacactag caaagtgaca gcaaacttcg ttaaagaaac
     1021 agcaagccca acatcaacct atgatacata tgttgaattt ggatttgcag gcagc
//