LOCUS       BBa_K1321356            1252 bp    DNA     linear       21-May-2024
DEFINITION  sfGFP fused to CBDclos driven by LacI
ACCESSION   BBa_K1321356
VERSION     BBa_K1321356.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..946
                     /label=sfGFP
     CDS             236..946
                     /label=BBa_K1321337
     misc_feature    947..952
                     /label=Scar 25
     misc_feature    947..952
                     /label=BBa_B0105
     CDS             953..1252
                     /label=BBa_K1321002
     CDS             236..1252
                     /label=BBa_K1321341
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggccgtaa
      241 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      301 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      361 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
      421 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      481 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
      541 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
      601 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
      661 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
      721 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
      781 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
      841 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
      901 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaaccg gctcatcaat
      961 gtcagttgaa ttttacaact ctaacaaatc agcacaaaca aactcaatta caccaataat
     1021 caaaattact aacacgtctg acagtgattt aaatttaaat gacgtaaaag ttagatatta
     1081 ttacacaagt gatggtacac aaggacaaac tttctggtgt gaccatgctg gtgcattatt
     1141 aggaaatagc tatgttgata acactagcaa agtgacagca aacttcgtta aagaaacagc
     1201 aagcccaaca tcaacctatg atacatatgt tgaatttgga tttgcaggca gc
//