BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDclos driven by LacI
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
mutable provenance: asdf
owned by: myers
owned by: james
mutable description: asdf
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: awef
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J04500
component: BBa_K1321341
component: BBa_K1319106
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321341
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
definition: BBa_J04500
access: public

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
definition: BBa_K1321341
access: public

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
definition: Scar25 ATG
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
type: Range
persistent identity: range2415481
display id: range2415481
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
type: Range
persistent identity: range2415483
display id: range2415483
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
type: Range
persistent identity: range2415489
display id: range2415489
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 236
end: 1252
orientation: inline

Scar25 ATG
type: ComponentDefinition
persistent identity: BBa K1319106
display id: BBa_K1319106
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Start Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
star: false
creator: Michael Osthege
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1319106_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2393317
display id: annotation2393317
version: 1
top level: Scar25 ATG
location: https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
type: Range
persistent identity: range2393317
display id: range2393317
version: 1
top level: Scar25 ATG
direction: direction/forward
start: 7
end: 9
orientation: inline

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDclos in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
mutable provenance: asdfad
owned by: james
owned by: myers
mutable description: asdfads
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdfd
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
definition: BBa_K1321337
access: public

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
definition: BBa_K1321002
access: public

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
definition: Scar 25
access: public

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 712
end: 717
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 718
end: 1017
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1321002
type: ComponentDefinition
persistent identity: BBa K1321002
display id: BBa_K1321002
version: 1
was generated by: iGEM to SBOL conversion
description: Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25
created: 2014-10-09T11:00:00Z
modified: 2015-06-17T12:35:41Z
mutable provenance: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
owned by: james
owned by: myers
mutable description: This part is a copy of BBa_K863111 (Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general.
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1321002_sequence/1

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP in Freiburg format (RFC 25)
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
mutable provenance: Made from existing part.
owned by: james
owned by: myers
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: OEPCR
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
top level: BBa_K1321337
direction: direction/forward
start: 1
end: 711
orientation: inline

https://synbiohub.org/public/igem/BBa_K1321356_sequence/1
type: Sequence
persistent identity: BBa K1321356 sequence
display id: BBa_K1321356_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319106_sequence/1
type: Sequence
persistent identity: BBa K1319106 sequence
display id: BBa_K1319106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321341_sequence/1
type: Sequence
persistent identity: BBa K1321341 sequence
display id: BBa_K1321341_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321002_sequence/1
type: Sequence
persistent identity: BBa K1321002 sequence
display id: BBa_K1321002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321337_sequence/1
type: Sequence
persistent identity: BBa K1321337 sequence
display id: BBa_K1321337_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z