##gff-version 3 ##sequence-region BBa_K1321358 1 825 BBa_K1321358 . CDS 650 825 . + 0 ID=BBa_K1321009;Name=BBa_K1321009 BBa_K1321358 . CDS 650 825 . + 0 ID=annotation2416941;Name=Nickel Binding Protein;Parent=BBa_K1321009 BBa_K1321358 . engineered_region 1 220 . + 0 ID=BBa_J04500;Name=BBa_J04500 BBa_K1321358 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500 BBa_K1321358 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K1321358 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K1321358 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K1321358 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K1321358 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K1321358 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K1321358 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K1321358 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500 BBa_K1321358 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1321358 . CDS 236 643 . + 0 ID=BBa_K1321339;Name=BBa_K1321339 BBa_K1321358 . CDS 236 553 . + 0 ID=annotation2418065;Name=CBDCenA;Parent=BBa_K1321339 BBa_K1321358 . CDS 554 643 . + 0 ID=annotation2414614;Name=Linker: Pro-Thr box;Parent=BBa_K1321339 BBa_K1321358 . sequence_feature 644 649 . + 0 ID=BBa_B0105;Name=BBa_B0105 BBa_K1321358 . sequence_feature 644 649 . + 0 ID=annotation2041621;Name=Scar 25;Parent=BBa_B0105 BBa_K1321358 . sequence_feature 221 235 . + 0 ID=BBa_K1319106;Name=BBa_K1319106 BBa_K1321358 . start_codon 227 229 . + 0 ID=annotation2393317;Name=start;Parent=BBa_K1319106 >BBa_K1321358 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcgc gccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctg ggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccg cgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtcttt cggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtacc ccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccga cggtcacccccaccggcgcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccac taccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatc atcaagaagaaggttgctgccacgggcatcacgag