LOCUS       BBa_K1321359            1318 bp    DNA     linear       22-Nov-2024
DEFINITION  sfGFP fused to dCBD driven by LacI
ACCESSION   BBa_K1321359
VERSION     BBa_K1321359.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..946
                     /label=sfGFP
     CDS             236..946
                     /label=BBa_K1321337
     misc_feature    947..952
                     /label=Scar 25
     misc_feature    947..952
                     /label=BBa_B0105
     CDS             953..1318
                     /label=dCBD
     CDS             953..1024
                     /label=Linker
     misc_feature    1025..1138
                     /label=cbh2 CBD
     CDS             1139..1210
                     /label=Linker
     misc_feature    1211..1318
                     /label=cbh1 CBD
     CDS             953..1318
                     /label=BBa_K1321340
     CDS             236..1318
                     /label=BBa_K1321348
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggccgtaa
      241 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      301 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      361 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
      421 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      481 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
      541 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
      601 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
      661 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
      721 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
      781 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
      841 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
      901 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaaccg gccccggcgc
      961 caatccgccc ggtactacga ccacttcgcg tccagcaacc acgaccgggt cgagccccgg
     1021 cccacaggcc tgttcttccg tctggggtca gtgcggcgga cagaactggt ctggccctac
     1081 ctgctgtgct tctggcagca catgcgtcta ctctaacgac tactatagcc agtgcctgcc
     1141 gggtgctaac ccgccaggca ctaccactac ctctcgcccc gctaccacta caggctctag
     1201 cccgggacct acccaaagcc actacggcca gtgcggaggc attggctaca gcggccctac
     1261 cgtctgcgcc tccggaacca cctgccaagt cctgaacccg tactactccc agtgtctt
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