LOCUS       BBa_K1321360            1252 bp    DNA     linear       22-Dec-2024
DEFINITION  CBDclos fused to sfGFP driven by LacI
ACCESSION   BBa_K1321360
VERSION     BBa_K1321360.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     misc_feature    221..235
                     /label=BBa_K1319106
     CDS             236..535
                     /label=BBa_K1321002
     misc_feature    536..541
                     /label=Scar 25
     misc_feature    536..541
                     /label=BBa_B0105
     CDS             542..1252
                     /label=sfGFP
     CDS             542..1252
                     /label=BBa_K1321337
     CDS             236..1252
                     /label=BBa_K1321346
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggctcatc
      241 aatgtcagtt gaattttaca actctaacaa atcagcacaa acaaactcaa ttacaccaat
      301 aatcaaaatt actaacacgt ctgacagtga tttaaattta aatgacgtaa aagttagata
      361 ttattacaca agtgatggta cacaaggaca aactttctgg tgtgaccatg ctggtgcatt
      421 attaggaaat agctatgttg ataacactag caaagtgaca gcaaacttcg ttaaagaaac
      481 agcaagccca acatcaacct atgatacata tgttgaattt ggatttgcag gcagcaccgg
      541 ccgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga
      601 tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa
      661 actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt
      721 aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca
      781 tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa
      841 ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa
      901 ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct
      961 ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat
     1021 taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca
     1081 ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct
     1141 gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct
     1201 ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca aa
//